Cloned (Comment) | Organism |
---|---|
gene ispE, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, genotyping. Multiple sequence analysis (MSA) of deduced amino acid sequences of PvIspE gene reveals that PvIspE amino acid sequences are regionally conserved as they show 100% similarity among all Indian isolates from a particular region. Specific mutations are found in different regions | Plasmodium vivax |
Protein Variants | Comment | Organism |
---|---|---|
A382T | naturally occuring mutation, neutral | Plasmodium vivax |
G402R | naturally occuring mutation, neutral | Plasmodium vivax |
K492T | naturally occuring mutation, neutral | Plasmodium vivax |
L405Q | naturally occuring mutation, neutral | Plasmodium vivax |
additional information | two non-synonymous mutations at codons 382 (A->T) and 492 (K->T) are found in all the isolates of Delhi, Chennai, Nadiad, Sonapur Goa, and Mangaluru. Apart from these, two more mutations are observed in all the isolates of Goa and Mangaluru namely one substitution at codon 351(T->M) and another synonymous mutation at codon 344 (S) TCT->TCC. In Rourkela which is one of the highly malaria endemic and isolated tribal regions of India, four non-synonymous mutations at codons 336 (R->S), 351 (T->M), 402 (G->R) and 405 (L->Q) are detected in all isolates. Domain architecture and mutation mapping, overview | Plasmodium vivax |
R336S | naturally occuring mutation, neutral | Plasmodium vivax |
T351M | naturally occuring mutation, neutral | Plasmodium vivax |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Plasmodium vivax |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol | Plasmodium vivax | - |
ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol | - |
? | |
ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol | Plasmodium vivax Salvador I | - |
ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Plasmodium vivax | A5KAU8 | clinical blood samples of Plasmodium vivax positive patients collected from seven malaria endemic geographical regions of India with entirely different topography viz. Delhi (New Delhi), Nadiad (Gujarat), Rourkela (Orissa), Goa (Goa), Mangaluru (Karnataka), Chennai (Tamil Nadu) and Sonapur (Assam) | - |
Plasmodium vivax Salvador I | A5KAU8 | clinical blood samples of Plasmodium vivax positive patients collected from seven malaria endemic geographical regions of India with entirely different topography viz. Delhi (New Delhi), Nadiad (Gujarat), Rourkela (Orissa), Goa (Goa), Mangaluru (Karnataka), Chennai (Tamil Nadu) and Sonapur (Assam) | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol | - |
Plasmodium vivax | ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol | - |
? | |
ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol | - |
Plasmodium vivax Salvador I | ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol | - |
? |
Synonyms | Comment | Organism |
---|---|---|
IspE | - |
Plasmodium vivax |
PvIspE | - |
Plasmodium vivax |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Plasmodium vivax |
General Information | Comment | Organism |
---|---|---|
additional information | three-dimensional structure and active site structure predictions. The active site of PvIspE consists of total 32 amino acid residues, 16 amino acid residues involved in ATP binding (Y225, P226, D230, N231, I232, K258, I262, F263, S264, G265, G267, G268, G269, N272, D304, S349) and 16 amino acid residues interacts with the substrate CDM (K136, N138, L141, H150, N151, M157, S270, G302, S303, D304, K319, S349, R350, Y353, L413, G444) | Plasmodium vivax |