Literature summary for 2.7.1.147 extracted from

Richter, J.P.; Goroncy, A.K.; Ronimus, R.S.; Sutherland-Smith, A.J.
The Structural and functional characterization of mammalian ADP-dependent glucokinase (2016), J. Biol. Chem., 291, 3694-3704.

Search for more literature for 2.7.1.147

Crystallization (Commentary)

Crystallization (Comment)	Organism
structures reveal a ribokinase-like tertiary fold similar to archaeal orthologues but with significant differences in some secondary structural elements. Both the unliganded and the AMP-bound ADPGK structures are in the open conformation and reveal the presence of a disulfide bond between conserved cysteines that is positioned at the nucleotide-binding loop. The AMP-bound structure defines the nucleotide-binding site with one of the disulfide bond cysteines coordinating the AMP with its main chain atoms	Homo sapiens

Crystallization (Comment)

Organism

structures reveal a ribokinase-like tertiary fold similar to archaeal orthologues but with significant differences in some secondary structural elements. Both the unliganded and the AMP-bound ADPGK structures are in the open conformation and reveal the presence of a disulfide bond between conserved cysteines that is positioned at the nucleotide-binding loop. The AMP-bound structure defines the nucleotide-binding site with one of the disulfide bond cysteines coordinating the AMP with its main chain atoms

Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
D481A	greatly reduced activity	Homo sapiens
D481E	greatly reduced activity	Homo sapiens
D481N	greatly reduced activity	Homo sapiens
D84A	greatly reduced activity	Homo sapiens
H264A	greatly reduced activity, no substrate inhibition with glucose concentrations of up to 55 mM	Homo sapiens
H382A	no catalytic activity	Homo sapiens
H382V/H387V	no catalytic activity	Homo sapiens
H387A	no catalytic activity	Homo sapiens
R228A	greatly reduced activity	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism
ADP	-	Homo sapiens
AMP	-	Homo sapiens
D-glucose	-	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism
0.23	-	ADP	mutant R228A, pH 7.0, 37°C	Homo sapiens
0.27	-	D-glucose	mutant R228A, pH 7.0, 37°C	Homo sapiens
0.48	-	D-glucose	pH 7.0, 37°C	Homo sapiens
0.56	-	ADP	pH 7.0, 37°C	Homo sapiens
2.9	-	GDP	pH 7.0, 37°C	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	Q9BRR6	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
ADP + D-glucose	-	Homo sapiens	AMP + D-glucose 6-phosphate	-	?
GDP + D-glucose	-	Homo sapiens	GMP + D-glucose 6-phosphate	-	?
additional information	poor activity with CDP. No substrates: ATP, 2-deoxyglucose, L-glucose, methyl-beta-D-xylopyranoside, D-glucosamine, L-rhamnose, D-tagatose, 3-O-methyl-D-glucopyranoside, 1-O-methyl-beta-D-glucopyranoside, 1-O-methyl-alpha-D-glucopyranoside, D-arabinose, L-arabinose, myo-inositol, D-ribose, D-xylose, D-galactose, D-fructose, D-mannose, D-fructose-6-phosphate, and alpha-D-glucose-1-phosphate	Homo sapiens	?	-	?

Synonyms

Synonyms	Comment	Organism
ADPGK	-	Homo sapiens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	-	Homo sapiens

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism
0.5	-	AMP	pH 7.0, 37°C	Homo sapiens
2.9	-	D-glucose	pH 7.0, 37°C	Homo sapiens
9.1	-	ADP	pH 7.0, 37°C	Homo sapiens