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Literature summary for 2.7.1.147 extracted from

  • Abarca-Lagunas, M.J.; Rivas-Pardo, J.A.; Ramirez-Sarmiento, C.A.; Guixe, V.
    Dissecting the functional roles of the conserved NXXE and HXE motifs of the ADP-dependent glucokinase from Thermococcus litoralis (2015), FEBS Lett., 589, 3271-3276.
    View publication on PubMed
Search for more literature for 2.7.1.147

Protein Variants

Protein Variants Comment Organism
E279D mutation does not affect the KM value for MgADP-, but causes a large increase on KM value for glucose and an 87fold weaker binding of glucose onto the non-hydrolysable enzyme-AMP-AlF3 complex. Mutant is still inhibited by free Mg2+ Thermococcus litoralis
E279L mutation does not affect the KM value for MgADP-, but causes a large increase on KM value for glucose and an 87fold weaker binding of glucose onto the non-hydrolysable enzyme-AMP-AlF3 complex. Mutant is still inhibited by free Mg2+ Thermococcus litoralis
E279Q mutation does not affect the KM value for MgADP-, but causes a large increase on KM value for glucose and an 87fold weaker binding of glucose onto the non-hydrolysable enzyme-AMP-AlF3 complex. Mutant is still inhibited by free Mg2+ Thermococcus litoralis
E308Q mutation increases the KM value for MgADP-, mutant is activated by free Mg2+ Thermococcus litoralis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.014
-
 ADP wild-type, pH 7.8, 40°C Thermococcus litoralis
0.034
-
 ADP mutant E279L, pH 7.8, 40°C Thermococcus litoralis
0.066
-
 ADP mutant E279Q, pH 7.8, 40°C Thermococcus litoralis
0.27
-
 ADP mutant E279D, pH 7.8, 40°C Thermococcus litoralis
0.28
-
 D-glucose wild-type, pH 7.8, 40°C Thermococcus litoralis
0.39
-
 D-glucose mutant E308Q, pH 7.8, 40°C Thermococcus litoralis
1.46
-
 ADP mutant E308Q, pH 7.8, 40°C Thermococcus litoralis
8
-
 D-glucose mutant E279D, pH 7.8, 40°C Thermococcus litoralis
8.9
-
 D-glucose mutant E279Q, pH 7.8, 40°C Thermococcus litoralis
109
-
 D-glucose mutant E279L, pH 7.8, 40°C Thermococcus litoralis

Organism

Organism UniProt Comment Textmining
Thermococcus litoralis Q7M537
-
-
Thermococcus litoralis DSM 5473 Q7M537
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP + D-glucose
-
 Thermococcus litoralis AMP + D-glucose 6-phosphate
-
 ?
ADP + D-glucose
-
 Thermococcus litoralis DSM 5473 AMP + D-glucose 6-phosphate
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.56
-
 ADP mutant E279L, pH 7.8, 40°C Thermococcus litoralis
0.71
-
 ADP mutant E279Q, pH 7.8, 40°C Thermococcus litoralis
1.52
-
 ADP mutant E279D, pH 7.8, 40°C Thermococcus litoralis
4.3
-
 ADP mutant E308Q, pH 7.8, 40°C Thermococcus litoralis
58
-
 ADP wild-type, pH 7.8, 40°C Thermococcus litoralis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.005
-
 D-glucose mutant E279L, pH 7.8, 40°C Thermococcus litoralis
0.079
-
 D-glucose mutant E279Q, pH 7.8, 40°C Thermococcus litoralis
0.19
-
 D-glucose mutant E279D, pH 7.8, 40°C Thermococcus litoralis
2.95
-
 ADP mutant E308Q, pH 7.8, 40°C Thermococcus litoralis
5.63
-
 ADP mutant E279D, pH 7.8, 40°C Thermococcus litoralis
10.7
-
 ADP mutant E279Q, pH 7.8, 40°C Thermococcus litoralis
11
-
 D-glucose mutant E308Q, pH 7.8, 40°C Thermococcus litoralis
16.4
-
 ADP mutant E279L, pH 7.8, 40°C Thermococcus litoralis
207
-
 D-glucose wild-type, pH 7.8, 40°C Thermococcus litoralis
4140
-
 ADP wild-type, pH 7.8, 40°C Thermococcus litoralis