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Literature summary for 2.7.1.146 extracted from

  • Gonzalez-Ordenes, F.; Cea, P.A.; Fuentes-Ugarte, N.; Munoz, S.M.; Zamora, R.A.; Leonardo, D.; Garratt, R.C.; Castro-Fernandez, V.; Guixe, V.
    ADP-dependent kinases from the archaeal order Methanosarcinales adapt to salt by a non-canonical evolutionarily conserved strategy (2018), Front. Microbiol., 9, 1305 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.1.146

Activating Compound

Activating Compound Comment Organism Structure
glycine betaine
-
 uncultured bacterium
glycine betaine activates at 0.1-1.5 M Methanosarcina mazei

Cloned(Commentary)

Cloned (Comment) Organism
gene pfkC, phylogenetic analysis Methanohalobium evestigatum
gene pfkC, phylogenetic analysis Methanosarcina mazei
phylogenetic analysis uncultured bacterium
phylogenetic analysis Pyrococcus horikoshii

Crystallization (Commentary)

Crystallization (Comment) Organism
purified enzyme, sitting drop vapor diffusion method, mixing of 0.001 ml of 10 mg/ml protein, 2.5 mM ADP, 200 mM NaCl, 7.5 mM MgCl2, 25 mM HEPES, pH 7.8, and 1 mM 2-mercaptoethanol with 0.001 ml of reservoir solution containing 20 mM CdCl2, 20 mM MgCl2, 20 mM NiCl2, 24% PEG MME 2000, and 100 mM sodium acetate, pH 4.5, 2 weeks, 19°C, X-ray diffraction structure determination and analysis at 1.46 A resolution, molecular replacement using the structure of the ancestral ADP-dependent kinase (PDB ID 5K27) split into small and large domains as search models Methanohalobium evestigatum
purified enzyme, sitting drop vapor diffusion method, mixing of 0.001 ml of 10 mg/ml protein, 2.5 mM ADP, 200 mM NaCl, 7.5 mM MgCl2, 25 mM HEPES, pH 7.8, and 1 mM 2-mercaptoethanol with 0.001 ml of reservoir solution containing 20 mM CdCl2, 20 mM MgCl2, 20 mM NiCl2, 24% PEG MME 2000, and 100 mM sodium acetate, pH 4.5, 2 weeks, 19°C, X-ray diffraction structure determination and analysis at 1.46 A resolution, molecular replacement using the structure of the ancestral ADP-dependent kinase (PDB ID 5K27) split into small and large domains as search models Methanosarcina mazei
purified enzyme, sitting drop vapor diffusion method, mixing of 0.001 ml of 10 mg/ml protein, 2.5 mM ADP, 200 mM NaCl, 7.5 mM MgCl2, 25 mM HEPES, pH 7.8, and 1 mM 2-mercaptoethanol with 0.001 ml of reservoir solution containing 20 mM CdCl2, 20 mM MgCl2, 20 mM NiCl2, 24% PEG MME 2000, and 100 mM sodium acetate, pH 4.5, 2 weeks, 19°C, X-ray diffraction structure determination and analysis at 2.86 A resolution, molecular replacement using the structure of the ancestral ADP-dependent kinase (PDB ID 5K27) split into small and large domains as search models uncultured bacterium

General Stability

General Stability Organism
enzyme MevePFK/GK protein is unstable at low KCl concentrations and is progressively stabilized at higher concentrations Methanohalobium evestigatum
enzyme MmazPFK/GK protein is unstable at low KCl concentrations and is progressively stabilized at higher concentrations Methanosarcina mazei

Inhibitors

Inhibitors Comment Organism Structure
ADP substrate inhibition Methanohalobium evestigatum
ADP substrate inhibition Methanosarcina mazei
ADP substrate inhibition uncultured bacterium
KCl inhibition by KCl is only partially prevented by glycine betaine Methanohalobium evestigatum
KCl activates at up to 0.5 M, inhibitory above about 1.0 M, inhibition by KCl is prevented by glycine betaine Methanosarcina mazei
NaCl moderate inhibition by 300 mM NaCl or higher concentration Methanohalobium evestigatum
NaCl moderate inhibition by 300 mM NaCl or higher concentration Methanosarcina mazei
NaCl moderate inhibition by 300 mM NaCl or higher concentration Pyrococcus horikoshii
NaCl moderate inhibition by 300 mM NaCl or higher concentration uncultured bacterium

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.006
-
 D-fructose 6-phosphate pH 6.5, 60°C uncultured bacterium
0.0066
-
 D-fructose 6-phosphate pH 6.5, 40°C Methanosarcina mazei
0.034
-
 D-fructose 6-phosphate pH 6.5, 40°C Methanohalobium evestigatum
0.072
-
 ADP MgADP, pH 6.5, 40°C Methanosarcina mazei
0.25
-
 ADP MgADP, pH 6.5, 40°C Methanohalobium evestigatum
0.7
-
 ADP MgADP, pH 6.5, 60°C uncultured bacterium

Metals/Ions

Metals/Ions Comment Organism Structure
KCl activates at up to 0.5 M, inhibitory above about 1.0 M Methanosarcina mazei
KCl has no evident effect on the AncMsPFK/GK activity uncultured bacterium
Mg2+ required uncultured bacterium
Mg2+ required Pyrococcus horikoshii
Mg2+ required Methanohalobium evestigatum
Mg2+ required Methanosarcina mazei

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ADP + D-fructose 6-phosphate uncultured bacterium
-
 AMP + D-fructose 1,6-bisphosphate
-
 ?
ADP + D-fructose 6-phosphate Pyrococcus horikoshii
-
 AMP + D-fructose 1,6-bisphosphate
-
 ?
ADP + D-fructose 6-phosphate Methanohalobium evestigatum
-
 AMP + D-fructose 1,6-bisphosphate
-
 ?
ADP + D-fructose 6-phosphate Methanosarcina mazei
-
 AMP + D-fructose 1,6-bisphosphate
-
 ?
ADP + D-fructose 6-phosphate Methanosarcina mazei OCM 88
-
 AMP + D-fructose 1,6-bisphosphate
-
 ?
ADP + D-fructose 6-phosphate Methanosarcina mazei DSM 3647
-
 AMP + D-fructose 1,6-bisphosphate
-
 ?
ADP + D-fructose 6-phosphate Methanosarcina mazei Goe1
-
 AMP + D-fructose 1,6-bisphosphate
-
 ?
ADP + D-fructose 6-phosphate Methanosarcina mazei JCM 11833
-
 AMP + D-fructose 1,6-bisphosphate
-
 ?
ADP + D-fructose 6-phosphate Methanohalobium evestigatum DSM 3721
-
 AMP + D-fructose 1,6-bisphosphate
-
 ?
ADP + D-fructose 6-phosphate Methanohalobium evestigatum OCM 161
-
 AMP + D-fructose 1,6-bisphosphate
-
 ?
ADP + D-fructose 6-phosphate Pyrococcus horikoshii ATCC 700860
-
 AMP + D-fructose 1,6-bisphosphate
-
 ?
ADP + D-fructose 6-phosphate Methanohalobium evestigatum Z-7303
-
 AMP + D-fructose 1,6-bisphosphate
-
 ?
ADP + D-fructose 6-phosphate Methanohalobium evestigatum ATCC BAA-1072
-
 AMP + D-fructose 1,6-bisphosphate
-
 ?
ADP + D-fructose 6-phosphate Methanohalobium evestigatum NBRC 107634
-
 AMP + D-fructose 1,6-bisphosphate
-
 ?
ADP + D-fructose 6-phosphate Methanosarcina mazei ATCC BAA-159
-
 AMP + D-fructose 1,6-bisphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Methanohalobium evestigatum D7E8P3
-
-
Methanohalobium evestigatum ATCC BAA-1072 D7E8P3
-
-
Methanohalobium evestigatum DSM 3721 D7E8P3
-
-
Methanohalobium evestigatum NBRC 107634 D7E8P3
-
-
Methanohalobium evestigatum OCM 161 D7E8P3
-
-
Methanohalobium evestigatum Z-7303 D7E8P3
-
-
Methanosarcina mazei Q8PZL9
-
-
Methanosarcina mazei ATCC BAA-159 Q8PZL9
-
-
Methanosarcina mazei DSM 3647 Q8PZL9
-
-
Methanosarcina mazei Goe1 Q8PZL9
-
-
Methanosarcina mazei JCM 11833 Q8PZL9
-
-
Methanosarcina mazei OCM 88 Q8PZL9
-
-
Pyrococcus horikoshii O59355
-
-
Pyrococcus horikoshii ATCC 700860 O59355
-
-
uncultured bacterium
-
 synthetic construct
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP + D-fructose 6-phosphate
-
 uncultured bacterium AMP + D-fructose 1,6-bisphosphate
-
 ?
ADP + D-fructose 6-phosphate
-
 Pyrococcus horikoshii AMP + D-fructose 1,6-bisphosphate
-
 ?
ADP + D-fructose 6-phosphate
-
 Methanohalobium evestigatum AMP + D-fructose 1,6-bisphosphate
-
 ?
ADP + D-fructose 6-phosphate
-
 Methanosarcina mazei AMP + D-fructose 1,6-bisphosphate
-
 ?
ADP + D-fructose 6-phosphate
-
 Methanosarcina mazei OCM 88 AMP + D-fructose 1,6-bisphosphate
-
 ?
ADP + D-fructose 6-phosphate
-
 Methanosarcina mazei DSM 3647 AMP + D-fructose 1,6-bisphosphate
-
 ?
ADP + D-fructose 6-phosphate
-
 Methanosarcina mazei Goe1 AMP + D-fructose 1,6-bisphosphate
-
 ?
ADP + D-fructose 6-phosphate
-
 Methanosarcina mazei JCM 11833 AMP + D-fructose 1,6-bisphosphate
-
 ?
ADP + D-fructose 6-phosphate
-
 Methanohalobium evestigatum DSM 3721 AMP + D-fructose 1,6-bisphosphate
-
 ?
ADP + D-fructose 6-phosphate
-
 Methanohalobium evestigatum OCM 161 AMP + D-fructose 1,6-bisphosphate
-
 ?
ADP + D-fructose 6-phosphate
-
 Pyrococcus horikoshii ATCC 700860 AMP + D-fructose 1,6-bisphosphate
-
 ?
ADP + D-fructose 6-phosphate
-
 Methanohalobium evestigatum Z-7303 AMP + D-fructose 1,6-bisphosphate
-
 ?
ADP + D-fructose 6-phosphate
-
 Methanohalobium evestigatum ATCC BAA-1072 AMP + D-fructose 1,6-bisphosphate
-
 ?
ADP + D-fructose 6-phosphate
-
 Methanohalobium evestigatum NBRC 107634 AMP + D-fructose 1,6-bisphosphate
-
 ?
ADP + D-fructose 6-phosphate
-
 Methanosarcina mazei ATCC BAA-159 AMP + D-fructose 1,6-bisphosphate
-
 ?
additional information the enzyme from Methanohalobium evestigatum also shows glucokinase activity, a bifunctional MevePFK/GK Methanohalobium evestigatum ?
-
-
additional information the enzyme from Methanosarcina mazei also shows glucokinase activity, a bifunctional AncMsPFK/GK uncultured bacterium ?
-
-
additional information the enzyme from Methanosarcina mazei also shows glucokinase activity, a bifunctional MmazPFK/GK Methanosarcina mazei ?
-
-
additional information the enzyme from Methanosarcina mazei also shows glucokinase activity, a bifunctional MmazPFK/GK Methanosarcina mazei OCM 88 ?
-
-
additional information the enzyme from Methanosarcina mazei also shows glucokinase activity, a bifunctional MmazPFK/GK Methanosarcina mazei DSM 3647 ?
-
-
additional information the enzyme from Methanosarcina mazei also shows glucokinase activity, a bifunctional MmazPFK/GK Methanosarcina mazei Goe1 ?
-
-
additional information the enzyme from Methanosarcina mazei also shows glucokinase activity, a bifunctional MmazPFK/GK Methanosarcina mazei JCM 11833 ?
-
-
additional information the enzyme from Methanohalobium evestigatum also shows glucokinase activity, a bifunctional MevePFK/GK Methanohalobium evestigatum DSM 3721 ?
-
-
additional information the enzyme from Methanohalobium evestigatum also shows glucokinase activity, a bifunctional MevePFK/GK Methanohalobium evestigatum OCM 161 ?
-
-
additional information the enzyme from Methanohalobium evestigatum also shows glucokinase activity, a bifunctional MevePFK/GK Methanohalobium evestigatum Z-7303 ?
-
-
additional information the enzyme from Methanohalobium evestigatum also shows glucokinase activity, a bifunctional MevePFK/GK Methanohalobium evestigatum ATCC BAA-1072 ?
-
-
additional information the enzyme from Methanohalobium evestigatum also shows glucokinase activity, a bifunctional MevePFK/GK Methanohalobium evestigatum NBRC 107634 ?
-
-
additional information the enzyme from Methanosarcina mazei also shows glucokinase activity, a bifunctional MmazPFK/GK Methanosarcina mazei ATCC BAA-159 ?
-
-

Synonyms

Synonyms Comment Organism
ADP-dependent phosphofructokinase
-
 uncultured bacterium
ADP-dependent phosphofructokinase
-
 Pyrococcus horikoshii
ADP-dependent phosphofructokinase
-
 Methanohalobium evestigatum
ADP-dependent phosphofructokinase
-
 Methanosarcina mazei
ADP-Pfk
-
 uncultured bacterium
ADP-Pfk
-
 Pyrococcus horikoshii
ADP-Pfk
-
 Methanohalobium evestigatum
ADP-Pfk
-
 Methanosarcina mazei
AncMsPFK/GK
-
 uncultured bacterium
MevePFK/GK
-
 Methanohalobium evestigatum
MmazPFK/GK
-
 Methanosarcina mazei
More see also EC 2.7.1.147 uncultured bacterium
More see also EC 2.7.1.147 Methanohalobium evestigatum
More see also EC 2.7.1.147 Methanosarcina mazei
pfkC
-
 Methanohalobium evestigatum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
 assay at Pyrococcus horikoshii
40
-
 assay at Methanohalobium evestigatum
40
-
 assay at, phosphofructokinase activity Methanosarcina mazei
60
-
 assay at uncultured bacterium

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.6
-
 D-fructose 6-phosphate pH 6.5, 40°C Methanosarcina mazei
6
-
 D-fructose 6-phosphate pH 6.5, 60°C uncultured bacterium
6.1
-
 ADP MgADP, pH 6.5, 40°C Methanosarcina mazei
8.7
-
 ADP MgADP, pH 6.5, 60°C uncultured bacterium
9.5
-
 D-fructose 6-phosphate pH 6.5, 40°C Methanohalobium evestigatum
16
-
 ADP MgADP, pH 6.5, 40°C Methanohalobium evestigatum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
 assay at uncultured bacterium
6.5
-
 assay at Pyrococcus horikoshii
6.5
-
 assay at Methanohalobium evestigatum
6.5
-
 assay at, phosphofructokinase activity Methanosarcina mazei

Cofactor

Cofactor Comment Organism Structure
ADP dependent on uncultured bacterium
ADP dependent on Pyrococcus horikoshii
ADP dependent on Methanohalobium evestigatum
ADP dependent on Methanosarcina mazei

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.003
-
 ADP MgADP, pH 6.5, 40°C Methanohalobium evestigatum
0.006
-
 ADP MgADP, pH 6.5, 60°C uncultured bacterium
0.0061
-
 ADP MgADP, pH 6.5, 40°C Methanosarcina mazei

General Information

General Information Comment Organism
evolution the enzyme belongs to the ADP-dependent phosphofructokinase/glucokinase family. Homology modeling of ADP-dependent sugar kinases from Halobacteria, Methanosarcinales and Eukarya, overview. Models built are divided into four groups based on the taxonomic categorization of the source organism and their ability to grow in high salinity environments as reported in the literature. The groups defined are: Halobacteria, halophilic Methanosarcinales, non-halophilic Methanosarcinales, and Eukarya, the latter used as a control outgroup. Analsis of enzyme ADP-PFK structure (PDB ID 1U2X) from Pyrococcus furiosus. Sequences and structures comparisons. Pyrococcus horikoshii posseses a phosphofructokinase and a glucokinase Pyrococcus horikoshii
evolution the enzyme belongs to the ADP-dependent phosphofructokinase/glucokinase family. Homology modeling of ADP-dependent sugar kinases from Halobacteria, Methanosarcinales and Eukarya, overview. Models built are divided into four groups based on the taxonomic categorization of the source organism and their ability to grow in high salinity environments as reported in the literature. The groups defined are: Halobacteria, halophilic Methanosarcinales, non-halophilic Methanosarcinales, and Eukarya, the latter used as a control outgroup. Sequences and structures comparisons uncultured bacterium
evolution the enzyme belongs to the ADP-dependent phosphofructokinase/glucokinase family. Homology modeling of ADP-dependent sugar kinases from Halobacteria, Methanosarcinales and Eukarya, overview. Models built are divided into four groups based on the taxonomic categorization of the source organism and their ability to grow in high salinity environments as reported in the literature. The groups defined are: Halobacteria, halophilic Methanosarcinales, non-halophilic Methanosarcinales, and Eukarya, the latter used as a control outgroup. Sequences and structures comparisons. Methanohalobium evestigatum posseses a bifunctional MevePFK/GK Methanohalobium evestigatum
evolution the enzyme belongs to the ADP-dependent phosphofructokinase/glucokinase family. Homology modeling of ADP-dependent sugar kinases from Halobacteria, Methanosarcinales and Eukarya, overview. Models built are divided into four groups based on the taxonomic categorization of the source organism and their ability to grow in high salinity environments as reported in the literature. The groups defined are: Halobacteria, halophilic Methanosarcinales, non-halophilic Methanosarcinales, and Eukarya, the latter used as a control outgroup. Sequences and structures comparisons. Methanosarcina mazei possesses a bifunctional MmazPFK/GK Methanosarcina mazei

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
12.43
-
 ADP MgADP, pH 6.5, 60°C uncultured bacterium
64
-
 ADP MgADP, pH 6.5, 40°C Methanohalobium evestigatum
84.7
-
 ADP MgADP, pH 6.5, 40°C Methanosarcina mazei
279.4
-
 D-fructose 6-phosphate pH 6.5, 40°C Methanohalobium evestigatum
697
-
 D-fructose 6-phosphate pH 6.5, 40°C Methanosarcina mazei
1000
-
 D-fructose 6-phosphate pH 6.5, 60°C uncultured bacterium