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Literature summary for 2.7.1.146 extracted from

  • Verhees, C.H.; Tuininga, J.E.; Kengen, S.W.; Stams, A.J.; van der Oost, J.; de Vos, W.M.
    ADP-dependent phosphofructokinases in mesophilic and thermophilic methanogenic archaea (2001), J. Bacteriol., 183, 7145-7153.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.1.146

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Methanocaldococcus jannaschii

Inhibitors

Inhibitors Comment Organism Structure
AMP 10 mM, 76% inhibition Methanocaldococcus jannaschii
ATP 10 mM, 47% inhibition Methanocaldococcus jannaschii
KCl 500 mM, 16% inhibition Methanocaldococcus jannaschii
additional information the addition of fructose 2,6-bisphosphate, pyruvate, glucose, phosphoenolpyruvate, or citrate to the assay mixture has no effect on the activity Methanocaldococcus jannaschii
NaCl 500 mM, 12% inhibition Methanocaldococcus jannaschii
Zn2+ no activity detected in presence of Zn2+ Methanocaldococcus jannaschii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0096
-
 D-fructose 6-phosphate pH 6.5, 50°C Methanocaldococcus jannaschii
0.49
-
 ADP pH 6.5, 50°C Methanocaldococcus jannaschii
11.9
-
 acetyl-phosphate pH 6.5, 50°C Methanocaldococcus jannaschii

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+
-
 Methanocaldococcus jannaschii
Ca2+ activity is highest in the presence of CaCl2, followed by MgCl2, Co2+ and Mn2+ Methanocaldococcus jannaschii
Co2+ activity is highest in the presence of CaCl2, followed by MgCl2, Co2+ and Mn2+ Methanocaldococcus jannaschii
Mg2+
-
 Methanocaldococcus jannaschii
Mg2+ activity is highest in the presence of CaCl2, followed by MgCl2, Co2+ and Mn2+ Methanocaldococcus jannaschii
Mn2+ activity is highest in the presence of CaCl2, followed by MgCl2, Co2+ and Mn2+ Methanocaldococcus jannaschii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50100
-
 native PAGE Methanocaldococcus jannaschii
50100
-
 non-denaturing PAGE Methanocaldococcus jannaschii
53362
-
 1 * 53362, calculated from sequence Methanocaldococcus jannaschii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ADP + D-fructose 6-phosphate Methanocaldococcus jannaschii key enzyme of the glycolytic pathway AMP + D-fructose 1,6-bisphosphate
-
 ?
ADP + D-fructose 6-phosphate Methanocaldococcus jannaschii DSM 2661 key enzyme of the glycolytic pathway AMP + D-fructose 1,6-bisphosphate
-
 ?
D-fructose 6-phosphate + ADP Methanocaldococcus jannaschii ADP can be replaced by acetylphosphate D-fructose 1,6-bisphosphate + AMP
-
 ?
D-fructose 6-phosphate + ADP Methanocaldococcus jannaschii ADP can be replaced by acetylphosphate D-fructose 1,6-bisphosphate + AMP
-
 ir

Organism

Organism UniProt Comment Textmining
Methanocaldococcus jannaschii
-
-
-
Methanocaldococcus jannaschii Q58999
-
-
Methanocaldococcus jannaschii DSM 2661 Q58999
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Methanocaldococcus jannaschii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
8.2
-
-
 Methanocaldococcus jannaschii
8.2
-
 pH 6.5, 50°C, direction of phosphorylation Methanocaldococcus jannaschii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-phosphate + D-fructose 6-phosphate activity is 83% compared to the activity with ADP Methanocaldococcus jannaschii acetate + D-fructose 1,6-bisphosphate
-
 ?
acetyl-phosphate + D-fructose 6-phosphate activity is 83% compared to the activity with ADP Methanocaldococcus jannaschii DSM 2661 acetate + D-fructose 1,6-bisphosphate
-
 ?
ADP + D-fructose 6-phosphate key enzyme of the glycolytic pathway Methanocaldococcus jannaschii AMP + D-fructose 1,6-bisphosphate
-
 ?
ADP + D-fructose 6-phosphate activity with GDP is 1.4% compared to the activity with ADP, activity with ATP is 0.3% compared to the activity with ADP, activity with GTP is 8.1% compared to the activity with ADP, no activity with diphosphate, phosphoenolpyruvate or polyphosphate Methanocaldococcus jannaschii AMP + D-fructose 1,6-bisphosphate
-
 ?
ADP + D-fructose 6-phosphate key enzyme of the glycolytic pathway Methanocaldococcus jannaschii DSM 2661 AMP + D-fructose 1,6-bisphosphate
-
 ?
ADP + D-fructose 6-phosphate activity with GDP is 1.4% compared to the activity with ADP, activity with ATP is 0.3% compared to the activity with ADP, activity with GTP is 8.1% compared to the activity with ADP, no activity with diphosphate, phosphoenolpyruvate or polyphosphate Methanocaldococcus jannaschii DSM 2661 AMP + D-fructose 1,6-bisphosphate
-
 ?
D-fructose 6-phosphate + ADP ADP can be replaced by acetylphosphate Methanocaldococcus jannaschii D-fructose 1,6-bisphosphate + AMP
-
 ?
D-fructose 6-phosphate + ADP ADP can be replaced by acetylphosphate Methanocaldococcus jannaschii D-fructose 1,6-bisphosphate + AMP
-
 ir

Subunits

Subunits Comment Organism
monomer 1 * 53362, calculated from sequence Methanocaldococcus jannaschii

Synonyms

Synonyms Comment Organism
ADP-dependent PFK
-
 Methanocaldococcus jannaschii
ADP-Pfk
-
 Methanocaldococcus jannaschii
MJ1604 MJ1604 Methanocaldococcus jannaschii
pfkC
-
 Methanocaldococcus jannaschii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
 assay at Methanocaldococcus jannaschii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
 assay at Methanocaldococcus jannaschii

General Information

General Information Comment Organism
physiological function key enzyme of the modified Embden-Meyerhof pathway of heterotrophic and chemolithoautotrophic archaea Methanocaldococcus jannaschii