BRENDA - Enzyme Database
show all sequences of 2.7.1.146

ADP-dependent phosphofructokinases in mesophilic and thermophilic methanogenic archaea

Verhees, C.H.; Tuininga, J.E.; Kengen, S.W.; Stams, A.J.; van der Oost, J.; de Vos, W.M.; J. Bacteriol. 183, 7145-7153 (2001)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression in Escherichia coli; expression in Escherichia coli
Methanocaldococcus jannaschii
Inhibitors
Inhibitors
Commentary
Organism
Structure
AMP
10 mM, 76% inhibition
Methanocaldococcus jannaschii
ATP
10 mM, 47% inhibition
Methanocaldococcus jannaschii
KCl
500 mM, 16% inhibition
Methanocaldococcus jannaschii
additional information
the addition of fructose 2,6-bisphosphate, pyruvate, glucose, phosphoenolpyruvate, or citrate to the assay mixture has no effect on the activity
Methanocaldococcus jannaschii
NaCl
500 mM, 12% inhibition
Methanocaldococcus jannaschii
Zn2+
no activity detected in presence of Zn2+
Methanocaldococcus jannaschii
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0096
-
D-fructose 6-phosphate
pH 6.5, 50°C
Methanocaldococcus jannaschii
0.49
-
ADP
pH 6.5, 50°C
Methanocaldococcus jannaschii
11.9
-
acetyl-phosphate
pH 6.5, 50°C
Methanocaldococcus jannaschii
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Ca2+
; activity is highest in the presence of CaCl2, followed by MgCl2, Co2+ and Mn2+
Methanocaldococcus jannaschii
Co2+
activity is highest in the presence of CaCl2, followed by MgCl2, Co2+ and Mn2+
Methanocaldococcus jannaschii
Mg2+
; activity is highest in the presence of CaCl2, followed by MgCl2, Co2+ and Mn2+
Methanocaldococcus jannaschii
Mn2+
activity is highest in the presence of CaCl2, followed by MgCl2, Co2+ and Mn2+
Methanocaldococcus jannaschii
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
50100
-
native PAGE; non-denaturing PAGE
Methanocaldococcus jannaschii
53362
-
1 * 53362, calculated from sequence
Methanocaldococcus jannaschii
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ADP + D-fructose 6-phosphate
Methanocaldococcus jannaschii
key enzyme of the glycolytic pathway
AMP + D-fructose 1,6-bisphosphate
-
-
?
ADP + D-fructose 6-phosphate
Methanocaldococcus jannaschii DSM 2661
key enzyme of the glycolytic pathway
AMP + D-fructose 1,6-bisphosphate
-
-
?
D-fructose 6-phosphate + ADP
Methanocaldococcus jannaschii
ADP can be replaced by acetylphosphate
D-fructose 1,6-bisphosphate + AMP
-
-
?
D-fructose 6-phosphate + ADP
Methanocaldococcus jannaschii
ADP can be replaced by acetylphosphate
D-fructose 1,6-bisphosphate + AMP
-
-
ir
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Methanocaldococcus jannaschii
-
-
-
Methanocaldococcus jannaschii
Q58999
-
-
Methanocaldococcus jannaschii DSM 2661
Q58999
-
-
Purification (Commentary)
Commentary
Organism
;
Methanocaldococcus jannaschii
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
8.2
-
; pH 6.5, 50°C, direction of phosphorylation
Methanocaldococcus jannaschii
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acetyl-phosphate + D-fructose 6-phosphate
activity is 83% compared to the activity with ADP
395269
Methanocaldococcus jannaschii
acetate + D-fructose 1,6-bisphosphate
-
-
-
?
acetyl-phosphate + D-fructose 6-phosphate
activity is 83% compared to the activity with ADP
395269
Methanocaldococcus jannaschii DSM 2661
acetate + D-fructose 1,6-bisphosphate
-
-
-
?
ADP + D-fructose 6-phosphate
key enzyme of the glycolytic pathway
395269
Methanocaldococcus jannaschii
AMP + D-fructose 1,6-bisphosphate
-
-
-
?
ADP + D-fructose 6-phosphate
activity with GDP is 1.4% compared to the activity with ADP, activity with ATP is 0.3% compared to the activity with ADP, activity with GTP is 8.1% compared to the activity with ADP, no activity with diphosphate, phosphoenolpyruvate or polyphosphate
395269
Methanocaldococcus jannaschii
AMP + D-fructose 1,6-bisphosphate
-
-
-
?
ADP + D-fructose 6-phosphate
key enzyme of the glycolytic pathway
395269
Methanocaldococcus jannaschii DSM 2661
AMP + D-fructose 1,6-bisphosphate
-
-
-
?
ADP + D-fructose 6-phosphate
activity with GDP is 1.4% compared to the activity with ADP, activity with ATP is 0.3% compared to the activity with ADP, activity with GTP is 8.1% compared to the activity with ADP, no activity with diphosphate, phosphoenolpyruvate or polyphosphate
395269
Methanocaldococcus jannaschii DSM 2661
AMP + D-fructose 1,6-bisphosphate
-
-
-
?
D-fructose 6-phosphate + ADP
ADP can be replaced by acetylphosphate
395269
Methanocaldococcus jannaschii
D-fructose 1,6-bisphosphate + AMP
-
-
-
?
D-fructose 6-phosphate + ADP
ADP can be replaced by acetylphosphate
395269
Methanocaldococcus jannaschii
D-fructose 1,6-bisphosphate + AMP
-
-
-
ir
Subunits
Subunits
Commentary
Organism
monomer
1 * 53362, calculated from sequence
Methanocaldococcus jannaschii
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
50
-
assay at
Methanocaldococcus jannaschii
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
-
assay at
Methanocaldococcus jannaschii
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Methanocaldococcus jannaschii
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
AMP
10 mM, 76% inhibition
Methanocaldococcus jannaschii
ATP
10 mM, 47% inhibition
Methanocaldococcus jannaschii
KCl
500 mM, 16% inhibition
Methanocaldococcus jannaschii
additional information
the addition of fructose 2,6-bisphosphate, pyruvate, glucose, phosphoenolpyruvate, or citrate to the assay mixture has no effect on the activity
Methanocaldococcus jannaschii
NaCl
500 mM, 12% inhibition
Methanocaldococcus jannaschii
Zn2+
no activity detected in presence of Zn2+
Methanocaldococcus jannaschii
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0096
-
D-fructose 6-phosphate
pH 6.5, 50°C
Methanocaldococcus jannaschii
0.49
-
ADP
pH 6.5, 50°C
Methanocaldococcus jannaschii
11.9
-
acetyl-phosphate
pH 6.5, 50°C
Methanocaldococcus jannaschii
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Ca2+
activity is highest in the presence of CaCl2, followed by MgCl2, Co2+ and Mn2+
Methanocaldococcus jannaschii
Ca2+
-
Methanocaldococcus jannaschii
Co2+
activity is highest in the presence of CaCl2, followed by MgCl2, Co2+ and Mn2+
Methanocaldococcus jannaschii
Mg2+
activity is highest in the presence of CaCl2, followed by MgCl2, Co2+ and Mn2+
Methanocaldococcus jannaschii
Mg2+
-
Methanocaldococcus jannaschii
Mn2+
activity is highest in the presence of CaCl2, followed by MgCl2, Co2+ and Mn2+
Methanocaldococcus jannaschii
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
50100
-
non-denaturing PAGE
Methanocaldococcus jannaschii
50100
-
native PAGE
Methanocaldococcus jannaschii
53362
-
1 * 53362, calculated from sequence
Methanocaldococcus jannaschii
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ADP + D-fructose 6-phosphate
Methanocaldococcus jannaschii
key enzyme of the glycolytic pathway
AMP + D-fructose 1,6-bisphosphate
-
-
?
ADP + D-fructose 6-phosphate
Methanocaldococcus jannaschii DSM 2661
key enzyme of the glycolytic pathway
AMP + D-fructose 1,6-bisphosphate
-
-
?
D-fructose 6-phosphate + ADP
Methanocaldococcus jannaschii
ADP can be replaced by acetylphosphate
D-fructose 1,6-bisphosphate + AMP
-
-
?
D-fructose 6-phosphate + ADP
Methanocaldococcus jannaschii
ADP can be replaced by acetylphosphate
D-fructose 1,6-bisphosphate + AMP
-
-
ir
Purification (Commentary) (protein specific)
Commentary
Organism
-
Methanocaldococcus jannaschii
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
8.2
-
pH 6.5, 50°C, direction of phosphorylation
Methanocaldococcus jannaschii
8.2
-
-
Methanocaldococcus jannaschii
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acetyl-phosphate + D-fructose 6-phosphate
activity is 83% compared to the activity with ADP
395269
Methanocaldococcus jannaschii
acetate + D-fructose 1,6-bisphosphate
-
-
-
?
acetyl-phosphate + D-fructose 6-phosphate
activity is 83% compared to the activity with ADP
395269
Methanocaldococcus jannaschii DSM 2661
acetate + D-fructose 1,6-bisphosphate
-
-
-
?
ADP + D-fructose 6-phosphate
key enzyme of the glycolytic pathway
395269
Methanocaldococcus jannaschii
AMP + D-fructose 1,6-bisphosphate
-
-
-
?
ADP + D-fructose 6-phosphate
activity with GDP is 1.4% compared to the activity with ADP, activity with ATP is 0.3% compared to the activity with ADP, activity with GTP is 8.1% compared to the activity with ADP, no activity with diphosphate, phosphoenolpyruvate or polyphosphate
395269
Methanocaldococcus jannaschii
AMP + D-fructose 1,6-bisphosphate
-
-
-
?
ADP + D-fructose 6-phosphate
key enzyme of the glycolytic pathway
395269
Methanocaldococcus jannaschii DSM 2661
AMP + D-fructose 1,6-bisphosphate
-
-
-
?
ADP + D-fructose 6-phosphate
activity with GDP is 1.4% compared to the activity with ADP, activity with ATP is 0.3% compared to the activity with ADP, activity with GTP is 8.1% compared to the activity with ADP, no activity with diphosphate, phosphoenolpyruvate or polyphosphate
395269
Methanocaldococcus jannaschii DSM 2661
AMP + D-fructose 1,6-bisphosphate
-
-
-
?
D-fructose 6-phosphate + ADP
ADP can be replaced by acetylphosphate
395269
Methanocaldococcus jannaschii
D-fructose 1,6-bisphosphate + AMP
-
-
-
?
D-fructose 6-phosphate + ADP
ADP can be replaced by acetylphosphate
395269
Methanocaldococcus jannaschii
D-fructose 1,6-bisphosphate + AMP
-
-
-
ir
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 53362, calculated from sequence
Methanocaldococcus jannaschii
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
50
-
assay at
Methanocaldococcus jannaschii
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
-
assay at
Methanocaldococcus jannaschii
General Information
General Information
Commentary
Organism
physiological function
key enzyme of the modified Embden-Meyerhof pathway of heterotrophic and chemolithoautotrophic archaea
Methanocaldococcus jannaschii
General Information (protein specific)
General Information
Commentary
Organism
physiological function
key enzyme of the modified Embden-Meyerhof pathway of heterotrophic and chemolithoautotrophic archaea
Methanocaldococcus jannaschii
Other publictions for EC 2.7.1.146
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
727486
Castro-Fernandez
Bifunctional ADP-dependent pho ...
Methanococcus maripaludis
FEBS J.
281
2017-2029
2014
1
-
1
1
-
-
6
6
-
5
-
2
-
1
-
-
1
-
-
-
-
-
11
-
-
-
-
5
2
2
-
-
4
-
4
1
-
1
-
1
-
-
4
6
4
6
-
5
-
2
-
-
-
1
-
-
-
-
11
-
-
-
-
5
2
2
-
-
-
-
-
-
6
6
721764
Merino
Catalytic and regulatory roles ...
Pyrococcus horikoshii
Biochimie
94
516-524
2012
-
-
-
-
-
-
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
3
-
-
-
-
-
-
-
-
3
3
698924
Currie
ADP-dependent 6-phosphofructok ...
Pyrococcus horikoshii, Pyrococcus horikoshii OT-3
J. Biol. Chem.
284
22664-22671
2009
-
-
1
1
15
-
3
30
-
7
-
1
-
6
-
-
1
-
-
-
-
-
11
-
1
-
-
16
-
-
-
5
-
-
-
-
-
2
6
2
29
-
-
3
-
58
-
7
-
1
-
-
-
2
-
-
-
-
11
-
1
-
-
30
-
-
-
-
-
1
1
-
30
30
686707
Merino
Specificity evolution of the A ...
Methanocaldococcus jannaschii
FEBS J.
275
4033-4044
2008
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
661677
Hansen
ADP-dependent 6-phosphofructok ...
Archaeoglobus fulgidus
Extremophiles
8
29-35
2004
1
-
-
-
-
-
1
6
1
6
3
1
-
4
-
-
1
-
-
-
2
1
3
2
1
1
2
-
1
1
-
2
-
-
-
1
-
-
2
-
-
-
-
1
-
6
1
6
3
1
-
-
-
1
-
-
2
1
3
2
1
1
2
-
1
1
-
-
-
-
-
-
-
-
660573
Jeong
Archaeal ADP-dependent phospho ...
Thermococcus litoralis
Acta Crystallogr. Sect. D
59
1327-1329
2003
-
-
1
1
-
-
-
1
-
1
2
1
-
3
-
-
1
-
-
-
1
-
1
1
-
-
-
-
-
-
-
2
-
-
-
-
-
1
2
1
-
-
-
-
-
1
-
1
2
1
-
-
-
1
-
-
1
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
395244
Sakuraba
ADP-dependent glucokinase/phos ...
Methanocaldococcus jannaschii
J. Biol. Chem.
277
12495-12498
2002
-
-
1
-
-
-
-
-
-
6
2
1
-
5
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
6
2
1
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
395242
Kengen
ADP-dependent glucokinase and ...
Pyrococcus furiosus
Methods Enzymol.
331
41-53
2001
-
-
1
-
-
-
2
-
-
2
2
1
-
1
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
2
-
-
-
2
2
1
-
-
-
1
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
395269
Verhees
ADP-dependent phosphofructokin ...
Methanocaldococcus jannaschii, Methanocaldococcus jannaschii DSM 2661
J. Bacteriol.
183
7145-7153
2001
-
-
1
-
-
-
6
3
-
4
2
4
-
13
-
-
1
-
-
-
1
-
8
1
1
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
6
-
3
-
6
3
4
-
-
-
2
-
-
2
-
8
1
1
-
-
-
1
-
-
-
-
1
1
-
-
-
395270
Ronimus
Sequencing, expression, charac ...
Thermococcus zilligii
Biochim. Biophys. Acta
1517
384-391
2001
-
-
1
-
-
-
-
-
-
-
3
1
-
7
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
3
1
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
721449
Labes
Sugar utilization in the hyper ...
Archaeoglobus fulgidus, Archaeoglobus fulgidus 7324
Arch. Microbiol.
176
329-338
2001
-
-
-
-
-
-
-
2
-
-
-
1
-
9
-
-
-
-
-
2
2
-
2
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
1
-
-
-
-
-
2
2
-
2
-
1
-
-
-
-
-
-
-
-
1
1
-
-
-
395266
Tuininga
Molecular and biochemical char ...
Pyrococcus furiosus
J. Biol. Chem.
274
21023-21028
1999
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