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Literature summary for 2.7.1.145 extracted from
- Mosquito has a single multisubstrate deoxyribonucleoside kinase characterized by unique substrate specificity (2003), Nucleic Acids Res., 31, 1665-1672.
Application
| Application | Comment | Organism |
|---|---|---|
| medicine | enzyme with increased specificity towards certain analogues can be used as a suicide gene in combined gene/chemotherapy treatment of cancer | Anopheles gambiae |
| synthesis | enzyme can be utilized in industrial large-scale production of dNTPs and analogues, used for DNA synthetic reactions, giving a higher yield and less toxic byproducts compared to chemical processes | Anopheles gambiae |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| DNA and amino acid sequence determination and analysis, overexpression in TK-deficient Escherichia coli strain KY895 as GST-fusion protein | Anopheles gambiae |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0058 | - |
2'-deoxythymidine | pH 8.0, recombinant enzyme | Anopheles gambiae |  |
| 0.0095 | - |
2'-deoxyguanosine | pH 8.0, recombinant enzyme | Anopheles gambiae | |
| 0.0126 | - |
2'-deoxycytidine | pH 8.0, recombinant enzyme | Anopheles gambiae | |
| 0.0181 | - |
2'-deoxyadenosine | pH 8.0, recombinant enzyme | Anopheles gambiae | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Anopheles gambiae | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 2'-deoxyadenosine | Anopheles gambiae | - |
ADP + 2'-deoxyadenosine 5'-phosphate | - |
? | |
| ATP + 2'-deoxycytidine | Anopheles gambiae | - |
ADP + 2'-deoxycytidine 5'-phosphate | - |
? | |
| ATP + 2'-deoxyguanosine | Anopheles gambiae | - |
ADP + 2'-deoxyguanosine 5'-phosphate | - |
? | |
| ATP + 2'-deoxythymidine | Anopheles gambiae | - |
ADP + 2'-deoxythymidine 5'-phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Anopheles gambiae | Q86LB8 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme from cell lines in a 5-step procedure, recombinant GST-fusion enzyme from Escherichia coli strain KY895 by GST-affinity chromatography | Anopheles gambiae |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| 4a-2s4 cell | - |
Anopheles gambiae | - |
| L3-5-3 cell | - |
Anopheles gambiae | - |
| Sua1.1 cell | - |
Anopheles gambiae | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
substrate specificities of cell lines | Anopheles gambiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + (E)-5-(2-bromovinyl)-2'-deoxyuridine | high activity | Anopheles gambiae | ADP + (E)-5-(2-bromovinyl)-2'-deoxyuridine 5'-phosphate | - |
? | |
| ATP + 1-beta-D-arabinofuranosylcytosine | low activity | Anopheles gambiae | ADP + 1-beta-D-arabinofuranosylcytosine 5'-phosphate | - |
? | |
| ATP + 1-beta-D-arabinofuranosylthymine | low activity | Anopheles gambiae | ADP + 1-beta-D-arabinofuranosylthymine 5'-phosphate | - |
? | |
| ATP + 2',3'-didehydro-3'-deoxythymidine | low activity | Anopheles gambiae | ADP + 2',3'-didehydro-3'-deoxythymidine 5'-phosphate | - |
? | |
| ATP + 2'-deoxyadenosine | - |
Anopheles gambiae | ADP + 2'-deoxyadenosine 5'-phosphate | - |
? | |
| ATP + 2'-deoxycytidine | - |
Anopheles gambiae | ADP + 2'-deoxycytidine 5'-phosphate | - |
? | |
| ATP + 2'-deoxycytidine | high activity | Anopheles gambiae | ADP + 2'-deoxycytidine 5'-phosphate | - |
? | |
| ATP + 2'-deoxyguanosine | - |
Anopheles gambiae | ADP + 2'-deoxyguanosine 5'-phosphate | - |
? | |
| ATP + 2'-deoxyguanosine | high activity | Anopheles gambiae | ADP + 2'-deoxyguanosine 5'-phosphate | - |
? | |
| ATP + 2'-deoxythymidine | - |
Anopheles gambiae | ADP + 2'-deoxythymidine 5'-phosphate | - |
? | |
| ATP + 2-chloro-2'-deoxyadenosine | best substrate | Anopheles gambiae | ADP + 2-chloro-2'-deoxyadenosine 5'-phosphate | - |
? | |
| ATP + 5-fluoro-2'-deoxyuridine | - |
Anopheles gambiae | ADP + 5-fluoro-2'-deoxyuridine 5'-phosphate | - |
? | |
| ATP + stavudine | anti-HIV nucleoside analogue | Anopheles gambiae | ADP + stavudine phosphate | - |
? | |
| additional information | substrate specificity, no activity with cytidine, adenine, guanine, and thymine, no activity with dideoxynucleosides, and with 3'-azido-2',3'-didehydro-3'-deoxythymidine | Anopheles gambiae | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| dNK | - |
Anopheles gambiae |
| multisubstrate deoxyribonucleoside kinase | - |
Anopheles gambiae |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.014 | - |
2'-deoxythymidine | pH 8.0, recombinant enzyme | Anopheles gambiae | |
| 0.019 | - |
2'-deoxyguanosine | pH 8.0, recombinant enzyme | Anopheles gambiae | |
| 0.021 | - |
2'-deoxyadenosine | pH 8.0, recombinant enzyme | Anopheles gambiae | |
| 0.03 | - |
2'-deoxycytidine | pH 8.0, recombinant enzyme | Anopheles gambiae | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Anopheles gambiae |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Anopheles gambiae | |
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