BRENDA - Enzyme Database show
show all sequences of 2.7.1.145

Structural basis for substrate specificities of cellular deoxyribonucleoside kinases

Johansson, K.; Ramaswamy, S.; Ljungcrantz, C.; Knecht, W.; Piskur, J.; Munch-Petersen, B.; Eriksson, S.; Eklund, H.; Nat. Struct. Biol. 8, 616-620 (2001)

Data extracted from this reference:

Application
Application
Commentary
Organism
medicine
the enzyme activates a number of medically important nucleoside analogues
Drosophila melanogaster
Crystallization (Commentary)
Crystallization
Organism
purified recombinant enzyme, hanging drop vapour diffusion method, enzyme solution containing 10 mg/ml protein and 10 mM deoxycytidine, mixed with an equal volume of cyrstallization solution containing 0.1 M MES, pH 6.5, 0.2 M ammonium sulfate, 20% w/v PEG 5000, and 8-10% v/v PEG 400, X-ray diffraction structure determination and analysis at 2.6 A resolution, molecular replacement method
Drosophila melanogaster
General Stability
General Stability
Organism
ATP stabilizes the enzyme structure
Drosophila melanogaster
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
-
Drosophila melanogaster
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Drosophila melanogaster
Q9XZT6
-
-
Reaction
Reaction
Commentary
Organism
ATP + a 2'-deoxyribonucleoside = ADP + a 2'-deoxyribonucleoside 5'-phosphate
substrate binding and interactions
Drosophila melanogaster
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + 2'-deoxycytidine
-
662932
Drosophila melanogaster
ADP + 2'-deoxycytidine 5'-phosphate
-
-
-
?
additional information
the enzyme shows broad substrate specificity probably due to a wide substrate binding cleft
662932
Drosophila melanogaster
?
-
-
-
-
Cofactor
Cofactor
Commentary
Organism
Structure
ATP
stabilizes the enzyme structure, binding structure, Q111 and Arg118 are involved
Drosophila melanogaster
Application (protein specific)
Application
Commentary
Organism
medicine
the enzyme activates a number of medically important nucleoside analogues
Drosophila melanogaster
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
ATP
stabilizes the enzyme structure, binding structure, Q111 and Arg118 are involved
Drosophila melanogaster
Crystallization (Commentary) (protein specific)
Crystallization
Organism
purified recombinant enzyme, hanging drop vapour diffusion method, enzyme solution containing 10 mg/ml protein and 10 mM deoxycytidine, mixed with an equal volume of cyrstallization solution containing 0.1 M MES, pH 6.5, 0.2 M ammonium sulfate, 20% w/v PEG 5000, and 8-10% v/v PEG 400, X-ray diffraction structure determination and analysis at 2.6 A resolution, molecular replacement method
Drosophila melanogaster
General Stability (protein specific)
General Stability
Organism
ATP stabilizes the enzyme structure
Drosophila melanogaster
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
-
Drosophila melanogaster
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + 2'-deoxycytidine
-
662932
Drosophila melanogaster
ADP + 2'-deoxycytidine 5'-phosphate
-
-
-
?
additional information
the enzyme shows broad substrate specificity probably due to a wide substrate binding cleft
662932
Drosophila melanogaster
?
-
-
-
-
Other publictions for EC 2.7.1.145
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
738739
Kamiya
In vivo selection of active de ...
Drosophila melanogaster
J. Biotechnol.
228
52-57
2016
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1
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1
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3
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1
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1
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1
1
-
-
-
738781
Slot Christiansen
Non-viral deoxyribonucleoside ...
Anopheles gambiae, Bombyx mori, Drosophila melanogaster
J. Genet. Genomics
42
235-248
2015
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2
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1
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3
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18
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3
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33
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3
1
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18
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33
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7
7
-
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739211
Jiang
Tanshinone IIA enhances bystan ...
Drosophila melanogaster
Oncol. Rep.
34
1487-1493
2015
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1
1
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4
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1
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3
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1
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3
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1
1
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737404
Serra
-
Immobilized Drosophila melanog ...
Drosophila melanogaster
Adv. Synth. Catal.
356
563-570
2014
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1
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1
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1
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1
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1
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14
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1
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1
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14
-
1
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1
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703396
Betham
Use of Drosophila deoxynucleos ...
Drosophila melanogaster
DNA Repair
9
153-160
2010
-
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1
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4
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1
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1
1
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721775
Ito
Cytotoxic effect of Drosophila ...
Drosophila melanogaster
Biol. Pharm. Bull.
33
1223-1227
2010
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1
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5
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702047
Knecht
Drosophila melanogaster deoxyr ...
Drosophila melanogaster
Biochem. Biophys. Res. Commun.
382
430-433
2009
-
-
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1
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1
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3
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1
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1
1
692289
Mikkelsen
Structural studies of nucleosi ...
Drosophila melanogaster
FEBS J.
275
2151-2160
2008
-
-
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1
-
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-
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2
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694476
Clausen
A multisubstrate deoxyribonucl ...
Arabidopsis thaliana
Nucleic Acids Symp. Ser.
52
489-490
2008
-
-
1
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-
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-
-
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2
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7
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1
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7
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673584
Egeblad-Welin
Functional studies of active-s ...
Drosophila melanogaster
FEBS J.
274
1542-1551
2007
-
-
-
1
7
-
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18
-
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2
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5
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18
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18
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5
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18
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673848
Knecht
Drosophila deoxyribonucleoside ...
Drosophila melanogaster
Gene Ther.
14
1278-1286
2007
-
1
1
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2
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4
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1
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675450
Gerth
Non-homologous recombination o ...
Drosophila melanogaster
J. Mol. Biol.
370
742-751
2007
-
-
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1
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1
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1
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1
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6
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1
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1
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1
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1
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-
6
-
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1
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676100
Solaroli
Mitochondrial expression of th ...
Drosophila melanogaster
Mol. Pharmacol.
72
1593-1598
2007
-
1
1
-
1
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-
-
2
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2
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672880
Jordheim
Gemcitabine resistance due to ...
Drosophila melanogaster
Cancer Chemother. Pharmacol.
58
547-554
2006
-
-
1
-
-
-
-
-
-
-
-
-
-
4
-
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1
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1
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672971
Legent
In vivo analysis of Drosophila ...
Drosophila melanogaster
Cell Cycle
5
740-749
2006
-
-
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1
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3
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1
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676247
Kamiya
Transient expression of Drosop ...
Drosophila melanogaster
Nucleosides Nucleotides Nucleic Acids
25
553-560
2006
-
1
1
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-
-
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3
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661702
Welin
Structural basis for the chang ...
Drosophila melanogaster
FEBS J.
272
3733-3742
2005
-
-
1
1
4
-
1
10
-
1
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4
-
2
-
-
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-
-
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1
-
7
-
-
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-
9
-
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-
1
4
-
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-
1
1
1
4
-
-
1
4
10
-
1
-
4
-
-
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-
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1
-
7
-
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-
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9
-
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661731
Piskur
Animal deoxyribonucleoside kin ...
Drosophila melanogaster
FEBS Lett.
560
3-6
2004
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1
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662990
Solaroli
Investigation of the substrate ...
Drosophila melanogaster
Nucleosides Nucleotides Nucleic Acids
23
1527-1529
2004
-
1
1
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3
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10
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1
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2
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1
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10
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1
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1
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1
1
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3
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10
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1
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1
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10
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1
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1
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660855
Krawiec
Inorganic tripolyphosphate (PP ...
Drosophila melanogaster
Biochem. Biophys. Res. Commun.
301
192-197
2003
-
-
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2
1
4
1
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1
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3
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4
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2
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1
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2
1
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1
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4
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661072
Mikkelsen
Structural basis for feedback ...
Drosophila melanogaster
Biochemistry
42
5706-5712
2003
-
-
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1
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1
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4
-
3
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5
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1
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1
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1
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4
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5
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661629
Solaroli
Active site mutants of Drosoph ...
Drosophila melanogaster
Eur. J. Biochem.
270
2879-2884
2003
-
-
1
-
9
-
-
9
-
-
-
-
-
3
-
-
1
-
-
-
1
-
9
-
1
-
-
-
1
-
-
1
-
-
-
-
-
1
1
-
9
-
-
-
-
9
-
-
-
-
-
-
-
1
-
-
1
-
9
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
662967
Knecht
Mosquito has a single multisub ...
Anopheles gambiae
Nucleic Acids Res.
31
1665-1672
2003
-
2
1
-
-
-
-
4
-
1
-
4
-
5
-
-
1
-
-
3
1
-
14
-
-
-
-
4
1
-
-
1
-
-
-
-
2
1
1
-
-
-
-
-
-
4
-
1
-
4
-
-
-
1
-
3
1
-
14
-
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-
-
4
1
-
-
-
-
-
-
-
-
-
662985
Krawiec
Ability of adenosine-2'(3')-de ...
Drosophila melanogaster
Nucleosides Nucleotides Nucleic Acids
22
1525-1529
2003
-
-
-
-
-
-
-
-
-
-
-
-
-
1
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-
-
-
-
2
-
10
-
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5
-
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5
-
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38
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