BRENDA - Enzyme Database show
show all sequences of 2.7.1.145

Deoxyribonucleoside kinases belonging to the thymidine kinase 2 (TK2)-like group vary significantly in substrate specificity, kinetics and feed-back regulation

Knecht, W.; Petersen, G.E.; Munch-Petersen, B.; Piskur, J.; J. Mol. Biol. 315, 529-540 (2002)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression as C-terminally His8-tagged protein in Escherichia coli strain BL21
Bombyx mori
DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression as full length and N-terminally truncated His-tagged enzymes in Escherichia coli
Xenopus laevis
DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression of wild-type and as C-terminally His8-tagged protein in Escherichia coli strain BL21
Drosophila melanogaster
Inhibitors
Inhibitors
Commentary
Organism
Structure
dCTP
competitive versus ATP, feedback inhibition
Xenopus laevis
dTTP
competitive versus ATP, feedback inhibition
Bombyx mori
dTTP
competitive versus ATP, feedback inhibition
Drosophila melanogaster
dTTP
competitive versus ATP, feedback inhibition
Xenopus laevis
additional information
no inhibition by dNTPs other than dTTP
Bombyx mori
additional information
no inhibition by dNTPs other than dTTP
Drosophila melanogaster
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
kinetics
Bombyx mori
additional information
-
additional information
kinetics
Xenopus laevis
0.0014
-
ATP
-
Bombyx mori
0.0025
-
ATP
-
Xenopus laevis
0.0061
-
2'-deoxycytidine
-
Bombyx mori
0.0153
-
2'-deoxythymidine
-
Bombyx mori
0.031
-
2'-deoxycytidine
-
Xenopus laevis
0.138
-
2'-deoxythymidine
-
Xenopus laevis
0.429
-
2'-deoxyguanosine
-
Bombyx mori
0.501
-
2'-deoxyadenosine
-
Bombyx mori
1.2
-
2'-deoxyguanosine
-
Xenopus laevis
2.1
-
2'-deoxyadenosine
-
Xenopus laevis
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
additional information
no sorting signals in the amino acid sequence
Bombyx mori
-
-
nucleus
enzyme possesses 2 potential nuclear targeting sequences
Drosophila melanogaster
5634
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
-
Bombyx mori
Mg2+
-
Xenopus laevis
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
26300
-
2 * 26300, recombinant enzyme, SDS-PAGE
Xenopus laevis
26900
-
2 * 29100, recombinant full length enzyme, SDS-PAGE, 2 * 26900, recombinant N-terminally truncated enzyme, SDS-PAGE
Drosophila melanogaster
29100
-
2 * 29100, recombinant full length enzyme, SDS-PAGE, 2 * 26900, recombinant N-terminally truncated enzyme, SDS-PAGE
Drosophila melanogaster
31300
-
2 * 31300, recombinant enzyme, SDS-PAGE
Bombyx mori
44600
-
recombinant N-terminally truncated enzyme, gel filtration
Drosophila melanogaster
46000
-
recombinant enzyme, native PAGE
Xenopus laevis
48500
-
recombinant full length enzyme, gel filtration
Drosophila melanogaster
49000
-
recombinant N-terminally truncated enzyme, native PAGE
Drosophila melanogaster
49800
-
recombinant enzyme, gel filtration
Xenopus laevis
57700
-
recombinant full length enzyme, native PAGE
Drosophila melanogaster
64400
-
recombinant enzyme, gel filtration
Bombyx mori
69300
-
recombinant enzyme, native PAGE
Bombyx mori
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ATP + 2'-deoxycytidine
Xenopus laevis
-
ADP + 2'-deoxycytidine 5'-phosphate
-
-
?
ATP + 2'-deoxythymidine
Xenopus laevis
-
ADP + 2'-deoxythymidine 5'-phosphate
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Bombyx mori
Q9BKL3
putative
-
Drosophila melanogaster
-
-
-
Xenopus laevis
Q8UVZ9
putative
-
Purification (Commentary)
Commentary
Organism
recombinant C-terminally His8-tagged enzyme from Escherichia coli strain BL21 by nickel affinity chromatography
Bombyx mori
recombinant C-terminally His8-tagged enzyme from Escherichia coli strain BL21 by nickel affinity chromatography, the His-tag is cleaved off by thrombin
Drosophila melanogaster
recombinant full length and N-terminally truncated His-tagged enzymes, the His-tags are cleaved off
Xenopus laevis
Source Tissue
Source Tissue
Commentary
Organism
Textmining
brain
-
Xenopus laevis
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
substrate specificity
Bombyx mori
additional information
-
substrate specificity
Xenopus laevis
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + 1-beta-D-arabinofuranosylcytosine
-
662607
Bombyx mori
ADP + 1-beta-D-arabinofuranosylcytosine 5'-phosphate
-
-
-
?
ATP + 1-beta-D-arabinofuranosylcytosine
-
662607
Xenopus laevis
ADP + 1-beta-D-arabinofuranosylcytosine 5'-phosphate
-
-
-
?
ATP + 1-beta-D-arabinofuranosylthymine
-
662607
Bombyx mori
ADP + 1-beta-D-arabinofuranosylthymine 5'-phosphate
-
-
-
?
ATP + 1-beta-D-arabinofuranosylthymine
-
662607
Xenopus laevis
ADP + 1-beta-D-arabinofuranosylthymine 5'-phosphate
-
-
-
?
ATP + 2',3'-didehydro-3'-deoxythymidine
-
662607
Bombyx mori
ADP + 2',3'-didehydro-3'-deoxythymidine 5'-phosphate
-
-
-
?
ATP + 2',3'-dideoxycytidine
-
662607
Bombyx mori
ADP + 2',3'-dideoxycytidine 5'-phosphate
-
-
-
?
ATP + 2',3'-dideoxycytidine
low activity
662607
Xenopus laevis
ADP + 2',3'-dideoxycytidine 5'-phosphate
-
-
-
?
ATP + 2',3'-dideoxythymidine
-
662607
Bombyx mori
ADP + 2',3'-dideoxythymidine 5'-phosphate
-
-
-
?
ATP + 2',3'-dideoxythymidine
low activity
662607
Xenopus laevis
ADP + 2',3'-dideoxythymidine 5'-phosphate
-
-
-
?
ATP + 2'-deoxyadenosine
-
662607
Bombyx mori
ADP + 2'-deoxyadenosine 5'-phosphate
-
-
-
?
ATP + 2'-deoxyadenosine
-
662607
Xenopus laevis
ADP + 2'-deoxyadenosine 5'-phosphate
-
-
-
?
ATP + 2'-deoxycytidine
-
662607
Bombyx mori
ADP + 2'-deoxycytidine 5'-phosphate
-
-
-
?
ATP + 2'-deoxycytidine
-
662607
Xenopus laevis
ADP + 2'-deoxycytidine 5'-phosphate
-
-
-
?
ATP + 2'-deoxyguanosine
-
662607
Bombyx mori
ADP + 2'-deoxyguanosine 5'-phosphate
-
-
-
?
ATP + 2'-deoxyguanosine
-
662607
Xenopus laevis
ADP + 2'-deoxyguanosine 5'-phosphate
-
-
-
?
ATP + 2'-deoxythymidine
-
662607
Xenopus laevis
ADP + 2'-deoxythymidine 5'-phosphate
-
-
-
?
ATP + 2'-deoxythymidine
best substrate
662607
Xenopus laevis
ADP + 2'-deoxythymidine 5'-phosphate
-
-
-
?
ATP + 2'-deoxythymidine
high activity
662607
Bombyx mori
ADP + 2'-deoxythymidine 5'-phosphate
-
-
-
?
ATP + 2-chloro-2'-deoxyadenosine
low activity
662607
Xenopus laevis
ADP + 2-chloro-2'-deoxyadenosine 5'-phosphate
-
-
-
?
ATP + 2-chloro-2'-deoxyadenosine
high activity
662607
Bombyx mori
ADP + 2-chloro-2'-deoxyadenosine 5'-phosphate
-
-
-
?
ATP + 3'-azido-2',3'-didehydro-3'-deoxythymidine
low activity
662607
Bombyx mori
ADP + 3'-azido-2',3'-didehydro-3'-deoxythymidine 5'-phosphate
-
-
-
?
ATP + 3'-azido-2',3'-didehydro-3'-deoxythymidine
low activity
662607
Xenopus laevis
ADP + 3'-azido-2',3'-didehydro-3'-deoxythymidine 5'-phosphate
-
-
-
?
ATP + 3'-deoxyadenosine
-
662607
Bombyx mori
ADP + 3'-deoxyadenosine 5'-phosphate
-
-
-
?
ATP + 5-fluoro-2'-deoxyuridine
high activity
662607
Bombyx mori
ADP + 5-fluoro-2'-deoxyuridine 5'-phosphate
-
-
-
?
ATP + 5-fluoro-2'-deoxyuridine
high activity
662607
Xenopus laevis
ADP + 5-fluoro-2'-deoxyuridine 5'-phosphate
-
-
-
?
ATP + 9-beta-D-arabinofuranosyladenine
-
662607
Bombyx mori
ADP + 9-beta-D-arabinofuranosyladenine 5'-phosphate
-
-
-
?
ATP + 9-beta-D-arabinofuranosyladenine
very low activity
662607
Xenopus laevis
ADP + 9-beta-D-arabinofuranosyladenine 5'-phosphate
-
-
-
?
ATP + aciclovir
-
662607
Bombyx mori
ADP + aciclovir phosphate
-
-
-
?
ATP + cytidine
-
662607
Bombyx mori
ADP + cytidine 5'-phosphate
-
-
-
?
ATP + cytidine
very low activity
662607
Xenopus laevis
ADP + cytidine 5'-phosphate
-
-
-
?
ATP + deoxyuridine
best substrate
662607
Bombyx mori
ADP + deoxyuridine 5'-phosphate
-
-
-
?
ATP + deoxyuridine
high activity
662607
Xenopus laevis
ADP + deoxyuridine 5'-phosphate
-
-
-
?
ATP + fludarabine
-
662607
Bombyx mori
ADP + fludarabine phosphate
-
-
-
?
ATP + stavudine
anti-HIV nucleoside analogue
662607
Bombyx mori
ADP + stavudine phosphate
-
-
-
?
ATP + uridine
very low activity
662607
Xenopus laevis
ADP + uridine 5'-phosphate
-
-
-
?
ATP + uridine
low activity
662607
Bombyx mori
ADP + uridine 5'-phosphate
-
-
-
?
additional information
substrate specificity
662607
Drosophila melanogaster
?
-
-
-
-
additional information
substrate specificity, no activity with 2-deoxyribose, dideoxyadenosine, dideoxyguanosine, adenosine, and guanosine
662607
Bombyx mori
?
-
-
-
-
additional information
substrate specificity, no activity with fludarabine, 3'-deoxyadenosine, 2',3'-didehydro-3'-deoxythymidine, 2-deoxyribose, dideoxyadenosine, dideoxyguanosine, adenosine, and guanosine
662607
Xenopus laevis
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
dimer
2 * 31300, recombinant enzyme, SDS-PAGE
Bombyx mori
dimer
2 * 29100, recombinant full length enzyme, SDS-PAGE, 2 * 26900, recombinant N-terminally truncated enzyme, SDS-PAGE
Drosophila melanogaster
dimer
2 * 26300, recombinant enzyme, SDS-PAGE
Xenopus laevis
More
quarternary structure determination
Bombyx mori
More
quarternary structure determination
Xenopus laevis
Cofactor
Cofactor
Commentary
Organism
Structure
ATP
-
Xenopus laevis
ATP
-
Bombyx mori
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
additional information
-
additional information
inhibition kinetics, overview
Bombyx mori
additional information
-
additional information
inhibition kinetics, overview
Drosophila melanogaster
additional information
-
additional information
inhibition kinetics, overview
Xenopus laevis
Cloned(Commentary) (protein specific)
Commentary
Organism
DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression as C-terminally His8-tagged protein in Escherichia coli strain BL21
Bombyx mori
DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression as full length and N-terminally truncated His-tagged enzymes in Escherichia coli
Xenopus laevis
DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression of wild-type and as C-terminally His8-tagged protein in Escherichia coli strain BL21
Drosophila melanogaster
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
ATP
-
Xenopus laevis
ATP
-
Bombyx mori
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
dCTP
competitive versus ATP, feedback inhibition
Xenopus laevis
dTTP
competitive versus ATP, feedback inhibition
Bombyx mori
dTTP
competitive versus ATP, feedback inhibition
Drosophila melanogaster
dTTP
competitive versus ATP, feedback inhibition
Xenopus laevis
additional information
no inhibition by dNTPs other than dTTP
Bombyx mori
additional information
no inhibition by dNTPs other than dTTP
Drosophila melanogaster
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
additional information
-
additional information
inhibition kinetics, overview
Bombyx mori
additional information
-
additional information
inhibition kinetics, overview
Drosophila melanogaster
additional information
-
additional information
inhibition kinetics, overview
Xenopus laevis
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
kinetics
Bombyx mori
additional information
-
additional information
kinetics
Xenopus laevis
0.0014
-
ATP
-
Bombyx mori
0.0025
-
ATP
-
Xenopus laevis
0.0061
-
2'-deoxycytidine
-
Bombyx mori
0.0153
-
2'-deoxythymidine
-
Bombyx mori
0.031
-
2'-deoxycytidine
-
Xenopus laevis
0.138
-
2'-deoxythymidine
-
Xenopus laevis
0.429
-
2'-deoxyguanosine
-
Bombyx mori
0.501
-
2'-deoxyadenosine
-
Bombyx mori
1.2
-
2'-deoxyguanosine
-
Xenopus laevis
2.1
-
2'-deoxyadenosine
-
Xenopus laevis
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
additional information
no sorting signals in the amino acid sequence
Bombyx mori
-
-
nucleus
enzyme possesses 2 potential nuclear targeting sequences
Drosophila melanogaster
5634
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
-
Bombyx mori
Mg2+
-
Xenopus laevis
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
26300
-
2 * 26300, recombinant enzyme, SDS-PAGE
Xenopus laevis
26900
-
2 * 29100, recombinant full length enzyme, SDS-PAGE, 2 * 26900, recombinant N-terminally truncated enzyme, SDS-PAGE
Drosophila melanogaster
29100
-
2 * 29100, recombinant full length enzyme, SDS-PAGE, 2 * 26900, recombinant N-terminally truncated enzyme, SDS-PAGE
Drosophila melanogaster
31300
-
2 * 31300, recombinant enzyme, SDS-PAGE
Bombyx mori
44600
-
recombinant N-terminally truncated enzyme, gel filtration
Drosophila melanogaster
46000
-
recombinant enzyme, native PAGE
Xenopus laevis
48500
-
recombinant full length enzyme, gel filtration
Drosophila melanogaster
49000
-
recombinant N-terminally truncated enzyme, native PAGE
Drosophila melanogaster
49800
-
recombinant enzyme, gel filtration
Xenopus laevis
57700
-
recombinant full length enzyme, native PAGE
Drosophila melanogaster
64400
-
recombinant enzyme, gel filtration
Bombyx mori
69300
-
recombinant enzyme, native PAGE
Bombyx mori
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ATP + 2'-deoxycytidine
Xenopus laevis
-
ADP + 2'-deoxycytidine 5'-phosphate
-
-
?
ATP + 2'-deoxythymidine
Xenopus laevis
-
ADP + 2'-deoxythymidine 5'-phosphate
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant C-terminally His8-tagged enzyme from Escherichia coli strain BL21 by nickel affinity chromatography
Bombyx mori
recombinant C-terminally His8-tagged enzyme from Escherichia coli strain BL21 by nickel affinity chromatography, the His-tag is cleaved off by thrombin
Drosophila melanogaster
recombinant full length and N-terminally truncated His-tagged enzymes, the His-tags are cleaved off
Xenopus laevis
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
brain
-
Xenopus laevis
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
substrate specificity
Bombyx mori
additional information
-
substrate specificity
Xenopus laevis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + 1-beta-D-arabinofuranosylcytosine
-
662607
Bombyx mori
ADP + 1-beta-D-arabinofuranosylcytosine 5'-phosphate
-
-
-
?
ATP + 1-beta-D-arabinofuranosylcytosine
-
662607
Xenopus laevis
ADP + 1-beta-D-arabinofuranosylcytosine 5'-phosphate
-
-
-
?
ATP + 1-beta-D-arabinofuranosylthymine
-
662607
Bombyx mori
ADP + 1-beta-D-arabinofuranosylthymine 5'-phosphate
-
-
-
?
ATP + 1-beta-D-arabinofuranosylthymine
-
662607
Xenopus laevis
ADP + 1-beta-D-arabinofuranosylthymine 5'-phosphate
-
-
-
?
ATP + 2',3'-didehydro-3'-deoxythymidine
-
662607
Bombyx mori
ADP + 2',3'-didehydro-3'-deoxythymidine 5'-phosphate
-
-
-
?
ATP + 2',3'-dideoxycytidine
-
662607
Bombyx mori
ADP + 2',3'-dideoxycytidine 5'-phosphate
-
-
-
?
ATP + 2',3'-dideoxycytidine
low activity
662607
Xenopus laevis
ADP + 2',3'-dideoxycytidine 5'-phosphate
-
-
-
?
ATP + 2',3'-dideoxythymidine
-
662607
Bombyx mori
ADP + 2',3'-dideoxythymidine 5'-phosphate
-
-
-
?
ATP + 2',3'-dideoxythymidine
low activity
662607
Xenopus laevis
ADP + 2',3'-dideoxythymidine 5'-phosphate
-
-
-
?
ATP + 2'-deoxyadenosine
-
662607
Bombyx mori
ADP + 2'-deoxyadenosine 5'-phosphate
-
-
-
?
ATP + 2'-deoxyadenosine
-
662607
Xenopus laevis
ADP + 2'-deoxyadenosine 5'-phosphate
-
-
-
?
ATP + 2'-deoxycytidine
-
662607
Bombyx mori
ADP + 2'-deoxycytidine 5'-phosphate
-
-
-
?
ATP + 2'-deoxycytidine
-
662607
Xenopus laevis
ADP + 2'-deoxycytidine 5'-phosphate
-
-
-
?
ATP + 2'-deoxyguanosine
-
662607
Bombyx mori
ADP + 2'-deoxyguanosine 5'-phosphate
-
-
-
?
ATP + 2'-deoxyguanosine
-
662607
Xenopus laevis
ADP + 2'-deoxyguanosine 5'-phosphate
-
-
-
?
ATP + 2'-deoxythymidine
-
662607
Xenopus laevis
ADP + 2'-deoxythymidine 5'-phosphate
-
-
-
?
ATP + 2'-deoxythymidine
best substrate
662607
Xenopus laevis
ADP + 2'-deoxythymidine 5'-phosphate
-
-
-
?
ATP + 2'-deoxythymidine
high activity
662607
Bombyx mori
ADP + 2'-deoxythymidine 5'-phosphate
-
-
-
?
ATP + 2-chloro-2'-deoxyadenosine
low activity
662607
Xenopus laevis
ADP + 2-chloro-2'-deoxyadenosine 5'-phosphate
-
-
-
?
ATP + 2-chloro-2'-deoxyadenosine
high activity
662607
Bombyx mori
ADP + 2-chloro-2'-deoxyadenosine 5'-phosphate
-
-
-
?
ATP + 3'-azido-2',3'-didehydro-3'-deoxythymidine
low activity
662607
Bombyx mori
ADP + 3'-azido-2',3'-didehydro-3'-deoxythymidine 5'-phosphate
-
-
-
?
ATP + 3'-azido-2',3'-didehydro-3'-deoxythymidine
low activity
662607
Xenopus laevis
ADP + 3'-azido-2',3'-didehydro-3'-deoxythymidine 5'-phosphate
-
-
-
?
ATP + 3'-deoxyadenosine
-
662607
Bombyx mori
ADP + 3'-deoxyadenosine 5'-phosphate
-
-
-
?
ATP + 5-fluoro-2'-deoxyuridine
high activity
662607
Bombyx mori
ADP + 5-fluoro-2'-deoxyuridine 5'-phosphate
-
-
-
?
ATP + 5-fluoro-2'-deoxyuridine
high activity
662607
Xenopus laevis
ADP + 5-fluoro-2'-deoxyuridine 5'-phosphate
-
-
-
?
ATP + 9-beta-D-arabinofuranosyladenine
-
662607
Bombyx mori
ADP + 9-beta-D-arabinofuranosyladenine 5'-phosphate
-
-
-
?
ATP + 9-beta-D-arabinofuranosyladenine
very low activity
662607
Xenopus laevis
ADP + 9-beta-D-arabinofuranosyladenine 5'-phosphate
-
-
-
?
ATP + aciclovir
-
662607
Bombyx mori
ADP + aciclovir phosphate
-
-
-
?
ATP + cytidine
-
662607
Bombyx mori
ADP + cytidine 5'-phosphate
-
-
-
?
ATP + cytidine
very low activity
662607
Xenopus laevis
ADP + cytidine 5'-phosphate
-
-
-
?
ATP + deoxyuridine
best substrate
662607
Bombyx mori
ADP + deoxyuridine 5'-phosphate
-
-
-
?
ATP + deoxyuridine
high activity
662607
Xenopus laevis
ADP + deoxyuridine 5'-phosphate
-
-
-
?
ATP + fludarabine
-
662607
Bombyx mori
ADP + fludarabine phosphate
-
-
-
?
ATP + stavudine
anti-HIV nucleoside analogue
662607
Bombyx mori
ADP + stavudine phosphate
-
-
-
?
ATP + uridine
very low activity
662607
Xenopus laevis
ADP + uridine 5'-phosphate
-
-
-
?
ATP + uridine
low activity
662607
Bombyx mori
ADP + uridine 5'-phosphate
-
-
-
?
additional information
substrate specificity
662607
Drosophila melanogaster
?
-
-
-
-
additional information
substrate specificity, no activity with 2-deoxyribose, dideoxyadenosine, dideoxyguanosine, adenosine, and guanosine
662607
Bombyx mori
?
-
-
-
-
additional information
substrate specificity, no activity with fludarabine, 3'-deoxyadenosine, 2',3'-didehydro-3'-deoxythymidine, 2-deoxyribose, dideoxyadenosine, dideoxyguanosine, adenosine, and guanosine
662607
Xenopus laevis
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 31300, recombinant enzyme, SDS-PAGE
Bombyx mori
dimer
2 * 29100, recombinant full length enzyme, SDS-PAGE, 2 * 26900, recombinant N-terminally truncated enzyme, SDS-PAGE
Drosophila melanogaster
dimer
2 * 26300, recombinant enzyme, SDS-PAGE
Xenopus laevis
More
quarternary structure determination
Bombyx mori
More
quarternary structure determination
Xenopus laevis
Other publictions for EC 2.7.1.145
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
738739
Kamiya
In vivo selection of active de ...
Drosophila melanogaster
J. Biotechnol.
228
52-57
2016
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1
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1
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3
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1
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1
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3
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1
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1
-
-
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1
1
-
-
-
738781
Slot Christiansen
Non-viral deoxyribonucleoside ...
Anopheles gambiae, Bombyx mori, Drosophila melanogaster
J. Genet. Genomics
42
235-248
2015
-
2
-
1
-
-
-
-
1
3
-
18
-
3
-
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33
-
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3
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2
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3
1
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1
3
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18
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33
-
-
-
-
-
-
-
-
-
-
7
7
-
-
-
739211
Jiang
Tanshinone IIA enhances bystan ...
Drosophila melanogaster
Oncol. Rep.
34
1487-1493
2015
-
1
1
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-
-
-
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4
-
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1
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3
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1
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3
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1
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1
1
1
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4
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1
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1
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-
3
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
737404
Serra
-
Immobilized Drosophila melanog ...
Drosophila melanogaster
Adv. Synth. Catal.
356
563-570
2014
-
1
-
-
1
-
-
-
-
1
-
1
-
1
-
-
-
-
-
-
-
-
14
-
1
-
-
-
1
-
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1
-
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-
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1
-
1
-
1
-
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-
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-
-
1
-
1
-
-
-
-
-
-
-
-
14
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
703396
Betham
Use of Drosophila deoxynucleos ...
Drosophila melanogaster
DNA Repair
9
153-160
2010
-
-
1
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
1
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-
-
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-
-
1
-
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1
1
-
-
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-
-
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-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
721775
Ito
Cytotoxic effect of Drosophila ...
Drosophila melanogaster
Biol. Pharm. Bull.
33
1223-1227
2010
-
-
1
-
-
-
-
-
-
-
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-
-
5
-
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-
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1
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-
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-
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
702047
Knecht
Drosophila melanogaster deoxyr ...
Drosophila melanogaster
Biochem. Biophys. Res. Commun.
382
430-433
2009
-
-
-
1
-
-
-
1
-
-
-
-
-
3
-
-
-
-
-
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-
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1
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1
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1
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-
1
1
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-
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1
-
-
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-
-
-
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-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
1
1
692289
Mikkelsen
Structural studies of nucleosi ...
Drosophila melanogaster
FEBS J.
275
2151-2160
2008
-
-
-
1
-
-
-
-
-
-
-
-
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2
-
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-
-
-
-
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-
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-
1
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
694476
Clausen
A multisubstrate deoxyribonucl ...
Arabidopsis thaliana
Nucleic Acids Symp. Ser.
52
489-490
2008
-
-
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
7
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
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-
-
-
-
-
-
-
7
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
673584
Egeblad-Welin
Functional studies of active-s ...
Drosophila melanogaster
FEBS J.
274
1542-1551
2007
-
-
-
1
7
-
-
18
-
-
-
-
-
2
-
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-
-
-
-
-
5
-
-
-
-
18
-
-
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-
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-
-
1
7
-
-
-
-
18
-
-
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
18
-
-
-
-
-
-
-
-
-
-
673848
Knecht
Drosophila deoxyribonucleoside ...
Drosophila melanogaster
Gene Ther.
14
1278-1286
2007
-
1
1
-
2
-
-
-
-
-
-
-
-
4
-
-
1
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1
1
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2
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1
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-
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-
-
-
-
-
-
-
675450
Gerth
Non-homologous recombination o ...
Drosophila melanogaster
J. Mol. Biol.
370
742-751
2007
-
-
-
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1
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1
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1
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1
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6
-
-
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-
1
-
-
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-
-
-
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-
-
-
-
1
-
-
-
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1
-
-
-
-
-
-
-
1
-
-
-
-
6
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
676100
Solaroli
Mitochondrial expression of th ...
Drosophila melanogaster
Mol. Pharmacol.
72
1593-1598
2007
-
1
1
-
1
-
-
-
2
-
-
-
-
2
-
-
-
-
-
-
-
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-
-
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-
-
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-
-
-
-
1
1
-
-
1
-
-
-
-
-
2
-
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
672880
Jordheim
Gemcitabine resistance due to ...
Drosophila melanogaster
Cancer Chemother. Pharmacol.
58
547-554
2006
-
-
1
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
672971
Legent
In vivo analysis of Drosophila ...
Drosophila melanogaster
Cell Cycle
5
740-749
2006
-
-
-
-
-
-
-
-
-
-
-
1
-
3
-
-
-
-
-
-
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1
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-
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1
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-
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1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
676247
Kamiya
Transient expression of Drosop ...
Drosophila melanogaster
Nucleosides Nucleotides Nucleic Acids
25
553-560
2006
-
1
1
-
-
-
-
-
-
-
-
-
-
3
-
-
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-
-
-
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1
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1
1
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-
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-
-
-
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-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
661702
Welin
Structural basis for the chang ...
Drosophila melanogaster
FEBS J.
272
3733-3742
2005
-
-
1
1
4
-
1
10
-
1
-
4
-
2
-
-
-
-
-
-
1
-
7
-
-
-
-
9
-
-
-
1
4
-
-
-
-
1
1
1
4
-
-
1
4
10
-
1
-
4
-
-
-
-
-
-
1
-
7
-
-
-
-
9
-
-
-
-
-
-
-
-
-
-
661731
Piskur
Animal deoxyribonucleoside kin ...
Drosophila melanogaster
FEBS Lett.
560
3-6
2004
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
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2
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1
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-
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2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
662990
Solaroli
Investigation of the substrate ...
Drosophila melanogaster
Nucleosides Nucleotides Nucleic Acids
23
1527-1529
2004
-
1
1
-
3
-
-
10
-
1
-
-
-
2
-
-
1
-
-
-
-
-
10
-
1
-
-
-
1
-
-
1
-
-
-
-
1
1
1
-
3
-
-
-
-
10
-
1
-
-
-
-
-
1
-
-
-
-
10
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
660855
Krawiec
Inorganic tripolyphosphate (PP ...
Drosophila melanogaster
Biochem. Biophys. Res. Commun.
301
192-197
2003
-
-
-
-
-
-
2
1
4
1
-
1
-
3
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
1
2
-
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-
1
-
-
-
-
2
2
1
4
1
-
1
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
661072
Mikkelsen
Structural basis for feedback ...
Drosophila melanogaster
Biochemistry
42
5706-5712
2003
-
-
-
1
-
-
1
-
-
-
-
4
-
3
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
1
-
-
-
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-
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1
-
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1
1
-
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4
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
661629
Solaroli
Active site mutants of Drosoph ...
Drosophila melanogaster
Eur. J. Biochem.
270
2879-2884
2003
-
-
1
-
9
-
-
9
-
-
-
-
-
3
-
-
1
-
-
-
1
-
9
-
1
-
-
-
1
-
-
1
-
-
-
-
-
1
1
-
9
-
-
-
-
9
-
-
-
-
-
-
-
1
-
-
1
-
9
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
662967
Knecht
Mosquito has a single multisub ...
Anopheles gambiae
Nucleic Acids Res.
31
1665-1672
2003
-
2
1
-
-
-
-
4
-
1
-
4
-
5
-
-
1
-
-
3
1
-
14
-
-
-
-
4
1
-
-
1
-
-
-
-
2
1
1
-
-
-
-
-
-
4
-
1
-
4
-
-
-
1
-
3
1
-
14
-
-
-
-
4
1
-
-
-
-
-
-
-
-
-
662985
Krawiec
Ability of adenosine-2'(3')-de ...
Drosophila melanogaster
Nucleosides Nucleotides Nucleic Acids
22
1525-1529
2003
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
2
-
10
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
10
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
661453
Eriksson
Structure and function of cell ...
Bombyx mori, Drosophila melanogaster
Cell. Mol. Life Sci.
59
1327-1346
2002
-
4
1
-
1
-
4
2
-
2
-
4
-
2
-
-
-
2
-
-
-
-
11
4
-
-
-
-
-
-
-
1
-
-
-
-
4
1
1
-
1
-
-
4
-
2
-
2
-
4
-
-
-
-
-
-
-
-
11
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
661583
Knecht
A few amino acid substitutions ...
Drosophila melanogaster
Embo J.
21
1873-1880
2002
-
-
1
-
8
-
-
38
-
1
-
1
-
6
-
-
1
-
-
-
1
-
7
-
-
-
-
38
-
-
-
1
-
-
-
-
-
1
1
-
8
-
-
-
-
38
-
1
-
1
-
-
-
1
-
-
1
-
7
-
-
-
-
38
-
-
-
-
-
-
-
-
-
-
662607
Knecht
Deoxyribonucleoside kinases be ...
Bombyx mori, Drosophila melanogaster, Xenopus laevis
J. Mol. Biol.
315
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