Application | Comment | Organism |
---|---|---|
medicine | enzyme with increased specificity towards certain analogues can be used as a suicide gene in combined gene/chemotherapy treatment of cancer | Bombyx mori |
medicine | enzyme with increased specificity towards certain analogues can be used as a suicide gene in combined gene/chemotherapy treatment of cancer | Drosophila melanogaster |
synthesis | enzyme can be utilized in industrial large-scale production of dNTPs and analogues, used for DNA synthetic reactions, giving a higher yield and less toxic byproducts compared to chemical processes | Drosophila melanogaster |
synthesis | enzyme can be utilized in industrial large-scale production of dNTPs and analogues, used forDNA synthetic reactions, giving a higher yield and less toxic byproducts compared to chemical processes | Bombyx mori |
Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, gene is located on chromosome 3 at position 91D-91E, expression of wild-type and mutant enzymes in Escherichia coli TK-deficient strain KY895 | Drosophila melanogaster |
Protein Variants | Comment | Organism |
---|---|---|
N45D/N64D | site-directed mutagenesis, mutant shows increased drug sensitivity and decreased inhibition by dTTP compared to the wild-type enzyme | Drosophila melanogaster |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
dTTP | competitive versus ATP, feedback inhibition | Bombyx mori | |
dTTP | competitive versus ATP, feedback inhibition | Drosophila melanogaster | |
additional information | no inhibition by dNTPs other than dTTP | Bombyx mori | |
additional information | no inhibition by dNTPs other than dTTP | Drosophila melanogaster |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | positive cooperativity with all 4 natural deoxyribonucleosides, no hyperbolic Michaelis-Menten substrate kinetics | Bombyx mori | |
additional information | - |
additional information | straight forward hyperbolic kinetic reaction mechanism following a compulsory ordered steady-state reaction mechanism with formation of a ternary complex | Drosophila melanogaster |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | dependent on | Bombyx mori | |
Mg2+ | dependent on | Drosophila melanogaster |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 2'-deoxyadenosine | Drosophila melanogaster | - |
ADP + 2'-deoxyadenosine 5'-phosphate | - |
? | |
ATP + 2'-deoxycytidine | Drosophila melanogaster | - |
ADP + 2'-deoxycytidine 5'-phosphate | - |
? | |
ATP + 2'-deoxyguanosine | Drosophila melanogaster | - |
ADP + 2'-deoxyguanosine 5'-phosphate | - |
? | |
ATP + 2'-deoxythymidine | Drosophila melanogaster | - |
ADP + 2'-deoxythymidine 5'-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bombyx mori | - |
- |
- |
Drosophila melanogaster | Q9XZT6 | dNK is the only deoxyriconucleoside kinase in the fruitfly | - |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + a 2'-deoxyribonucleoside = ADP + a 2'-deoxyribonucleoside 5'-phosphate | positive cooperativity mechanism, substrate and nucleotide interactions and binding sites, overview | Bombyx mori | |
ATP + a 2'-deoxyribonucleoside = ADP + a 2'-deoxyribonucleoside 5'-phosphate | straight forward hyperbolic kinetic reaction mechanism following a compulsory ordered steady-state reaction mechanism with formation of a ternary complex, substrate interactions | Drosophila melanogaster |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 1-beta-D-arabinofuranosylcytosine | - |
Drosophila melanogaster | ADP + 1-beta-D-arabinofuranosylcytosine 5'-phosphate | - |
? | |
ATP + 1-beta-D-arabinofuranosylthymine | - |
Drosophila melanogaster | ADP + 1-beta-D-arabinofuranosylthymine 5'-phosphate | - |
? | |
ATP + 2'-deoxyadenosine | - |
Drosophila melanogaster | ADP + 2'-deoxyadenosine 5'-phosphate | - |
? | |
ATP + 2'-deoxycytidine | - |
Drosophila melanogaster | ADP + 2'-deoxycytidine 5'-phosphate | - |
? | |
ATP + 2'-deoxyguanosine | - |
Drosophila melanogaster | ADP + 2'-deoxyguanosine 5'-phosphate | - |
? | |
ATP + 2'-deoxythymidine | - |
Drosophila melanogaster | ADP + 2'-deoxythymidine 5'-phosphate | - |
? | |
ATP + 2-chloro-2'-deoxyadenosine | - |
Drosophila melanogaster | ADP + 2-chloro-2'-deoxyadenosine 5'-phosphate | - |
? | |
ATP + 5-fluoro-2'-deoxyuridine | - |
Drosophila melanogaster | ADP + 5-fluoro-2'-deoxyuridine 5'-phosphate | - |
? | |
ATP + E-5-(2-bromovinyl)-2'-deoxyuridine | - |
Drosophila melanogaster | ADP + E-5-(2-bromovinyl)-2'-deoxyuridine phosphate | - |
? | |
additional information | broad substrate specificity | Drosophila melanogaster | ? | - |
? | |
additional information | substrate specificity, no activity with purine ribonucleosides and dideoxynucleosides | Bombyx mori | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | - |
Bombyx mori |
dimer | - |
Drosophila melanogaster |
More | ternary and quarternary structure analysis, overview | Bombyx mori |
More | ternary and quarternary structure analysis, overview | Drosophila melanogaster |
Synonyms | Comment | Organism |
---|---|---|
dNK | - |
Bombyx mori |
dNK | - |
Drosophila melanogaster |
More | the enzyme belongs to the thymidine kinase 2-like, i.e. TK2-like, family of enzymes | Bombyx mori |
More | the enzyme belongs to the thymidine kinase 2-like, i.e. TK2-like, family of enzymes | Drosophila melanogaster |
multisubstrate deoxyribonucleoside kinase | - |
Bombyx mori |
multisubstrate deoxyribonucleoside kinase | - |
Drosophila melanogaster |
nucleoside triphosphate: deoxyribonucleoside 5'-phosphotransferase | - |
Drosophila melanogaster |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Drosophila melanogaster |