BRENDA - Reference to 2.7.1.145; Id = 661453

medicine

enzyme with increased specificity towards certain analogues can be used as a suicide gene in combined gene/chemotherapy treatment of cancer

Bombyx mori

medicine

enzyme with increased specificity towards certain analogues can be used as a suicide gene in combined gene/chemotherapy treatment of cancer

Drosophila melanogaster

synthesis

enzyme can be utilized in industrial large-scale production of dNTPs and analogues, used forDNA synthetic reactions, giving a higher yield and less toxic byproducts compared to chemical processes

Bombyx mori

synthesis

enzyme can be utilized in industrial large-scale production of dNTPs and analogues, used for DNA synthetic reactions, giving a higher yield and less toxic byproducts compared to chemical processes

Drosophila melanogaster

DNA and amino acid sequence determination and analysis, gene is located on chromosome 3 at position 91D-91E, expression of wild-type and mutant enzymes in Escherichia coli TK-deficient strain KY895

Drosophila melanogaster

N45D/N64D

site-directed mutagenesis, mutant shows increased drug sensitivity and decreased inhibition by dTTP compared to the wild-type enzyme

Drosophila melanogaster

dTTP

competitive versus ATP, feedback inhibition

Bombyx mori

dTTP

competitive versus ATP, feedback inhibition

Drosophila melanogaster

additional information

no inhibition by dNTPs other than dTTP

Bombyx mori

additional information

no inhibition by dNTPs other than dTTP

Drosophila melanogaster

additional information

-

additional information

positive cooperativity with all 4 natural deoxyribonucleosides, no hyperbolic Michaelis-Menten substrate kinetics

Bombyx mori

additional information

-

additional information

straight forward hyperbolic kinetic reaction mechanism following a compulsory ordered steady-state reaction mechanism with formation of a ternary complex

Drosophila melanogaster

Mg2+

dependent on

Bombyx mori

Mg2+

dependent on

Drosophila melanogaster

ATP + 2'-deoxyadenosine

Drosophila melanogaster

-

ADP + 2'-deoxyadenosine 5'-phosphate

-

?

ATP + 2'-deoxycytidine

Drosophila melanogaster

-

ADP + 2'-deoxycytidine 5'-phosphate

-

?

ATP + 2'-deoxyguanosine

Drosophila melanogaster

-

ADP + 2'-deoxyguanosine 5'-phosphate

-

?

ATP + 2'-deoxythymidine

Drosophila melanogaster

-

ADP + 2'-deoxythymidine 5'-phosphate

-

?

Bombyx mori

-

Drosophila melanogaster

Q9XZT6

dNK is the only deoxyriconucleoside kinase in the fruitfly

-

ATP + a 2'-deoxyribonucleoside = ADP + a 2'-deoxyribonucleoside 5'-phosphate

positive cooperativity mechanism, substrate and nucleotide interactions and binding sites, overview

Bombyx mori

ATP + a 2'-deoxyribonucleoside = ADP + a 2'-deoxyribonucleoside 5'-phosphate

straight forward hyperbolic kinetic reaction mechanism following a compulsory ordered steady-state reaction mechanism with formation of a ternary complex, substrate interactions

Drosophila melanogaster

ATP + 1-beta-D-arabinofuranosylcytosine

-

661453

Drosophila melanogaster

ADP + 1-beta-D-arabinofuranosylcytosine 5'-phosphate

-

?

ATP + 1-beta-D-arabinofuranosylthymine

-

661453

Drosophila melanogaster

ADP + 1-beta-D-arabinofuranosylthymine 5'-phosphate

-

?

ATP + 2'-deoxyadenosine

-

661453

Drosophila melanogaster

ADP + 2'-deoxyadenosine 5'-phosphate

-

?

ATP + 2'-deoxycytidine

-

661453

Drosophila melanogaster

ADP + 2'-deoxycytidine 5'-phosphate

-

?

ATP + 2'-deoxyguanosine

-

661453

Drosophila melanogaster

ADP + 2'-deoxyguanosine 5'-phosphate

-

?

ATP + 2'-deoxythymidine

-

661453

Drosophila melanogaster

ADP + 2'-deoxythymidine 5'-phosphate

-

?

ATP + 2-chloro-2'-deoxyadenosine

-

661453

Drosophila melanogaster

ADP + 2-chloro-2'-deoxyadenosine 5'-phosphate

-

?

ATP + 5-fluoro-2'-deoxyuridine

-

661453

Drosophila melanogaster

ADP + 5-fluoro-2'-deoxyuridine 5'-phosphate

-

?

ATP + E-5-(2-bromovinyl)-2'-deoxyuridine

-

661453

Drosophila melanogaster

ADP + E-5-(2-bromovinyl)-2'-deoxyuridine phosphate

-

?

additional information

broad substrate specificity

661453

Drosophila melanogaster

?

-

additional information

substrate specificity, no activity with purine ribonucleosides and dideoxynucleosides

661453

Bombyx mori

?

-

dimer

-

Bombyx mori

dimer

-

Drosophila melanogaster

More

ternary and quarternary structure analysis, overview

Bombyx mori

More

ternary and quarternary structure analysis, overview

Drosophila melanogaster

dNK

-

Bombyx mori

dNK

-

Drosophila melanogaster

More

the enzyme belongs to the thymidine kinase 2-like, i.e. TK2-like, family of enzymes

Bombyx mori

More

the enzyme belongs to the thymidine kinase 2-like, i.e. TK2-like, family of enzymes

Drosophila melanogaster

multisubstrate deoxyribonucleoside kinase

-

Bombyx mori

multisubstrate deoxyribonucleoside kinase

-

Drosophila melanogaster

nucleoside triphosphate: deoxyribonucleoside 5'-phosphotransferase

-

Drosophila melanogaster

ATP

-

Drosophila melanogaster

medicine

enzyme with increased specificity towards certain analogues can be used as a suicide gene in combined gene/chemotherapy treatment of cancer

Bombyx mori

medicine

enzyme with increased specificity towards certain analogues can be used as a suicide gene in combined gene/chemotherapy treatment of cancer

Drosophila melanogaster

synthesis

enzyme can be utilized in industrial large-scale production of dNTPs and analogues, used forDNA synthetic reactions, giving a higher yield and less toxic byproducts compared to chemical processes

Bombyx mori

synthesis

enzyme can be utilized in industrial large-scale production of dNTPs and analogues, used for DNA synthetic reactions, giving a higher yield and less toxic byproducts compared to chemical processes

Drosophila melanogaster

DNA and amino acid sequence determination and analysis, gene is located on chromosome 3 at position 91D-91E, expression of wild-type and mutant enzymes in Escherichia coli TK-deficient strain KY895

Drosophila melanogaster

ATP

-

Drosophila melanogaster

N45D/N64D

site-directed mutagenesis, mutant shows increased drug sensitivity and decreased inhibition by dTTP compared to the wild-type enzyme

Drosophila melanogaster

dTTP

competitive versus ATP, feedback inhibition

Bombyx mori

dTTP

competitive versus ATP, feedback inhibition

Drosophila melanogaster

additional information

no inhibition by dNTPs other than dTTP

Bombyx mori

additional information

no inhibition by dNTPs other than dTTP

Drosophila melanogaster

additional information

-

additional information

positive cooperativity with all 4 natural deoxyribonucleosides, no hyperbolic Michaelis-Menten substrate kinetics

Bombyx mori

additional information

-

additional information

straight forward hyperbolic kinetic reaction mechanism following a compulsory ordered steady-state reaction mechanism with formation of a ternary complex

Drosophila melanogaster

Mg2+

dependent on

Bombyx mori

Mg2+

dependent on

Drosophila melanogaster

ATP + 2'-deoxyadenosine

Drosophila melanogaster

-

ADP + 2'-deoxyadenosine 5'-phosphate

-

?

ATP + 2'-deoxycytidine

Drosophila melanogaster

-

ADP + 2'-deoxycytidine 5'-phosphate

-

?

ATP + 2'-deoxyguanosine

Drosophila melanogaster

-

ADP + 2'-deoxyguanosine 5'-phosphate

-

?

ATP + 2'-deoxythymidine

Drosophila melanogaster

-

ADP + 2'-deoxythymidine 5'-phosphate

-

?

ATP + 1-beta-D-arabinofuranosylcytosine

-

661453

Drosophila melanogaster

ADP + 1-beta-D-arabinofuranosylcytosine 5'-phosphate

-

?

ATP + 1-beta-D-arabinofuranosylthymine

-

661453

Drosophila melanogaster

ADP + 1-beta-D-arabinofuranosylthymine 5'-phosphate

-

?

ATP + 2'-deoxyadenosine

-

661453

Drosophila melanogaster

ADP + 2'-deoxyadenosine 5'-phosphate

-

?

ATP + 2'-deoxycytidine

-

661453

Drosophila melanogaster

ADP + 2'-deoxycytidine 5'-phosphate

-

?

ATP + 2'-deoxyguanosine

-

661453

Drosophila melanogaster

ADP + 2'-deoxyguanosine 5'-phosphate

-

?

ATP + 2'-deoxythymidine

-

661453

Drosophila melanogaster

ADP + 2'-deoxythymidine 5'-phosphate

-

?

ATP + 2-chloro-2'-deoxyadenosine

-

661453

Drosophila melanogaster

ADP + 2-chloro-2'-deoxyadenosine 5'-phosphate

-

?

ATP + 5-fluoro-2'-deoxyuridine

-

661453

Drosophila melanogaster

ADP + 5-fluoro-2'-deoxyuridine 5'-phosphate

-

?

ATP + E-5-(2-bromovinyl)-2'-deoxyuridine

-

661453

Drosophila melanogaster

ADP + E-5-(2-bromovinyl)-2'-deoxyuridine phosphate

-

?

additional information

broad substrate specificity

661453

Drosophila melanogaster

?

-

additional information

substrate specificity, no activity with purine ribonucleosides and dideoxynucleosides

661453

Bombyx mori

?

-

dimer

-

Bombyx mori

dimer

-

Drosophila melanogaster

More

ternary and quarternary structure analysis, overview

Bombyx mori

More

ternary and quarternary structure analysis, overview

Drosophila melanogaster

738739

Kamiya

In vivo selection of active de ...

Drosophila melanogaster

J. Biotechnol.

228

52-57

2016

-

1

-

1

-

1

-

1

-

3

-

1

-

3

-

2

1

-

1

-

1

-

1

-

1

-

1

-

1

-

1

-

3

-

1

-

1

-

1

-

738781

Slot Christiansen

-

Non-viral deoxyribonucleoside ...

Anopheles gambiae, Bombyx mori, Drosophila melanogaster

J. Genet. Genomics

42

235-248

2015

-

2

-

1

-

1

3

-

18

-

3

-

33

-

6

-

3

-

2

-

3

1

-

1

3

-

18

-

33

-

7

-

739211

Jiang

Tanshinone IIA enhances bystan ...

Drosophila melanogaster

Oncol. Rep.

34

1487-1493

2015

-

1

-

4

-

1

-

3

-

1

-

3

-

2

-

1

-

1

-

4

-

1

-

1

-

3

-

1

-

737404

Serra

-

Immobilized Drosophila melanog ...

Drosophila melanogaster

Adv. Synth. Catal.

356

563-570

2014

-

1

-

1

-

1

-

1

-

1

-

14

-

1

-

1

-

1

-

1

-

1

-

1

-

1

-

1

-

14

-

1

-

1

-

703396

Betham

Use of Drosophila deoxynucleos ...

Drosophila melanogaster

DNA Repair

9

153-160

2010

-

1

-

4

-

1

-

1

-

1

-

1

-

1

-

1

-

721775

Ito

Cytotoxic effect of Drosophila ...

Drosophila melanogaster

Biol. Pharm. Bull.

33

1223-1227

2010

-

1

-

5

-

2

-

1

-

702047

Knecht

Drosophila melanogaster deoxyr ...

Drosophila melanogaster

Biochem. Biophys. Res. Commun.

382

430-433

2009

-

1

-

1

-

3

-

1

-

1

-

1

-

1

-

1

-

1

-

1

-

1

-

1

692289

Mikkelsen

Structural studies of nucleosi ...

Drosophila melanogaster

FEBS J.

275

2151-2160

2008

-

1

-

2

-

1

-

694476

Clausen

A multisubstrate deoxyribonucl ...

Arabidopsis thaliana

Nucleic Acids Symp. Ser.

52

489-490

2008

-

1

-

2

-

7

-

1

-

1

-

7

-

673584

Egeblad-Welin

Functional studies of active-s ...

Drosophila melanogaster

FEBS J.

274

1542-1551

2007

-

1

7

-

18

-

2

-

5

-

18

-

1

7

-

18

-

5

-

18

-

673848

Knecht

Drosophila deoxyribonucleoside ...

Drosophila melanogaster

Gene Ther.

14

1278-1286

2007

-

1

-

2

-

4

-

1

-

1

-

1

-

2

-

1

-

675450

Gerth

Non-homologous recombination o ...

Drosophila melanogaster

J. Mol. Biol.

370

742-751

2007

-

1

-

1

-

1

-

1

-

6

-

1

-

1

-

1

-

1

-

6

-

1

-

676100

Solaroli

Mitochondrial expression of th ...

Drosophila melanogaster

Mol. Pharmacol.

72

1593-1598

2007

-

1

-

1

-

2

-

2

-

2

-

1

-

1

-

2

-

672880

Jordheim

Gemcitabine resistance due to ...

Drosophila melanogaster

Cancer Chemother. Pharmacol.

58

547-554

2006

-

1

-

4

-

1

-

1

-

1

-

1

-

672971

Legent

In vivo analysis of Drosophila ...

Drosophila melanogaster

Cell Cycle

5

740-749

2006

-

1

-

3

-

1

-

1

-

1

-

1

-

676247

Kamiya

Transient expression of Drosop ...

Drosophila melanogaster

Nucleosides Nucleotides Nucleic Acids

25

553-560

2006

-

1

-

3

-

1

-

1

-

1

-

661702

Welin

Structural basis for the chang ...

Drosophila melanogaster

FEBS J.

272

3733-3742

2005

-

1

4

-

1

10

-

1

-

4

-

2

-

1

-

7

-

1

-

9

-

1

4

-

1

4

-

1

4

10

-

1

-

4

-

1

-

7

-

9

-

661731

Piskur

Animal deoxyribonucleoside kin ...

Drosophila melanogaster

FEBS Lett.

560

3-6

2004

-

1

-

3

-

2

-

2

-

1

-

2

-

662990

Solaroli

Investigation of the substrate ...

Drosophila melanogaster

Nucleosides Nucleotides Nucleic Acids

23

1527-1529

2004

-

1

-

3

-

10

-

1

-

2

-

1

-

10

-

2

1

-

1

-

1

-

1

-

3

-

10

-

1

-

1

-

10

-

1

-

1

-

660855

Krawiec

Inorganic tripolyphosphate (PP ...

Drosophila melanogaster

Biochem. Biophys. Res. Commun.

301

192-197

2003

-

2

1

4

1

-

1

-

3

-

4

-

1

-

1

2

-

1

-

2

1

4

1

-

1

-

4

-

661072

Mikkelsen

Structural basis for feedback ...

Drosophila melanogaster

Biochemistry

42

5706-5712

2003

-

1

-

1

-

4

-

3

-

5

-

2

-

1

-

1

-

1

-

4

-

5

-

661629

Solaroli

Active site mutants of Drosoph ...

Drosophila melanogaster

Eur. J. Biochem.

270

2879-2884

2003

-

1

-

9

-

9

-

3

-

1

-

1

-

9

-

2

1

-

1

-

1

-

1

-

9

-

9

-

1

-

1

-

9

-

1

-

1

-

662967

Knecht

Mosquito has a single multisub ...

Anopheles gambiae

Nucleic Acids Res.

31

1665-1672

2003

-

2

1

-

4

-

1

-

4

-

5

-

1

-

3

1

-

14

-

2

-

4

1

-

1

-

2

1

-

4

-

1

-

4

-

1

-

3

1

-

14

-

4

1

-

662985

Krawiec

Ability of adenosine-2'(3')-de ...

Drosophila melanogaster

Nucleosides Nucleotides Nucleic Acids

22

1525-1529

2003

-

1

-

2

-

10

-

1

-

5

-

5

-

2

-

10

-

661453

Eriksson

Structure and function of cell ...

Bombyx mori, Drosophila melanogaster

Cell. Mol. Life Sci.

59

1327-1346

2002

-

4

1

-

1

-

4

2

-

2

-

4

-

2

-

2

-

11

4

7

-

1

-

4

1

-

1

-

4

-

2

-

2

-

4

-

11

4

-

661583

Knecht

A few amino acid substitutions ...

Drosophila melanogaster

Embo J.

21

1873-1880

2002

-

1

-

8

-

38

-

1

-

1

-

6

-

1

-

1

-

7

-

2

-

38

-

1

-

1

-

8

-

38

-

1

-

1

-

1

-

1

-

7

-

38

-

662607

Knecht

Deoxyribonucleoside kinases be ...

Bombyx mori, Drosophila melanogaster, Xenopus laevis

J. Mol. Biol.

315

529-540

2002

-

3

-

6

12

2

12

2

-

8

-

3

-

1

2

-

39

5

9

-

2

3

-

3

2

-

6

3

12

2

12

2

-

3

-

1

2

-

39

5

-

660852

Zheng

Nucleoside analog cytotoxicity ...

Drosophila melanogaster

Biochem. Biophys. Res. Commun.

289

229-233

2001

-

1

-

2

-

1

-

3

-

1

-

2

-

1

-

1

-

2

-

1

-

3

-

1

-

1

-

662932

Johansson

Structural basis for substrate ...

Drosophila melanogaster

Nat. Struct. Biol.

8

616-620

2001

-

1

-

1

-

1

-

1

-

3

-

1

-

2

-

1

-

1

-

1

-

1

-

1

-

1

-

2

-

636842

Munch-Petersen

Functional expression of a mul ...

Drosophila melanogaster

J. Biol. Chem.

275

6673-6679

2000

-

1

-

1

-

4

-

1

-

5

-

1

-

4

-

18

1

-

4

-

1

-

1

-

4

-

1

-

1

-

4

-

18

1

-

4

-

636843

Balzarini

The multifunctional deoxynucle ...

Drosophila melanogaster

Mol. Pharmacol.

57

811-819

2000

-

1

-

5

1

-

1

-

4

-

3

-

1

-

3

-

1

-

3

5

1

-

1

-

3

-

636844

Knecht

Identification of residues inv ...

Drosophila melanogaster

J. Mol. Biol.

301

827-837

2000

-

11

-

1

8

-

3

-

17

-

12

-

11

-

1

-

8

-

17

-

12

-

636841

Johansson

Cloning and characterization o ...

Drosophila melanogaster

J. Biol. Chem.

274

23814-23819

1999

-

1

-

17

4

-

1

-

4

-

4

1

-

15

-

1

-

15

17

-

4

-

1

-

4

1

-

636839

Munch-Petersen

The single deoxynucleoside kin ...

Drosophila melanogaster, Drosophila melanogaster S-2

Adv. Exp. Med. Biol.

431

465-469

1998

-

4

-

2

-

4

-

1

-

1

-

15

1

-

1

-

3

-

4

3

4

-

2

-

1

-

1

-

15

1

-

1

-

636840

Munch-Petersen

Four deoxynucleoside kinase ac ...

Drosophila melanogaster, Drosophila melanogaster S-2

J. Biol. Chem.

273

3926-3931

1998

-

4

5

-

2

-

5

-

1

-

1

-

21

1

-

1

-

4

-

8

-

4

8

5

-

2

-

1

-

1

-

21

1

-

4

-

Structure and function of cellular deoxyribonucleoside kinases

Data extracted from this reference: