Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2'-deoxyadenosine | reaction with thymidine or 2'-deoxyguanosine | Drosophila melanogaster | |
2'-deoxycytidine | competitive with thymidine | Drosophila melanogaster | |
2'-deoxyguanosine | reaction with thymidine or 2'-deoxyadenosine | Drosophila melanogaster | |
thymidine | strong inhibitor of reaction with 2'-deoxycytidine, 2'-deoxyguanosine or 2'-deoxyadenosine | Drosophila melanogaster |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0009 | - |
thymidine | pH 8.0, 22°C | Drosophila melanogaster | |
0.001 | - |
2'-deoxycytidine | pH 8.0, 22°C | Drosophila melanogaster | |
0.028 | - |
1-beta-D-arabinofuranosylcytosine | - |
Drosophila melanogaster | |
0.109 | - |
2'-deoxyadenosine | pH 8.0, 22°C | Drosophila melanogaster | |
0.654 | - |
2'-deoxyguanosine | pH 8.0, 22°C | Drosophila melanogaster |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30000 | - |
1 * 30000, SDS-PAGE | Drosophila melanogaster |
33000 | - |
gel filtration | Drosophila melanogaster |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Drosophila melanogaster | - |
S-2 | - |
Drosophila melanogaster S-2 | - |
S-2 | - |
Purification (Comment) | Organism |
---|---|
- |
Drosophila melanogaster |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + a 2'-deoxyribonucleoside = ADP + a 2'-deoxyribonucleoside 5'-phosphate | compulsory ordered steady-state reaction mechanism with formation of a ternary complex with the phosphate donor and acceptor | Drosophila melanogaster |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
embryo | - |
Drosophila melanogaster | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
29.4 | - |
- |
Drosophila melanogaster |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-beta-D-arabinofuranosylcytosine + ATP | 10% of the activity with thymidine and 2'-deoxycytidine | Drosophila melanogaster | 1-beta-D-arabinofuranosylcytosine 5'-phosphate + ADP | - |
? | |
1-beta-D-arabinofuranosylthymine + ATP | 15% of the activity with thymidine and 2'-deoxycytidine | Drosophila melanogaster | 1-beta-D-arabinofuranosylthymine 5'-phosphate + ADP | - |
? | |
2',3'-dideoxycytidine + ATP | 0.02% of the activity with thymidine and 2'-deoxycytidine | Drosophila melanogaster | 2',3'-dideoxycytidine 5'-phosphate + ADP | - |
? | |
2'-deoxyadenosine + ATP | 5% of the activity with thymidine and 2'-deoxycytidine | Drosophila melanogaster | 2'-deoxyadenosine 5'-phosphate + ADP | - |
? | |
2'-deoxycytidine + ATP | - |
Drosophila melanogaster | 2'-deoxycytidine 5'-phosphate + ADP | - |
? | |
2'-deoxycytidine + ATP | - |
Drosophila melanogaster S-2 | 2'-deoxycytidine 5'-phosphate + ADP | - |
? | |
2'-deoxycytidine + CTP | - |
Drosophila melanogaster | 2'-deoxycytidine 5'-phosphate + CDP | - |
? | |
2'-deoxyguanosine + ATP | 1% of the activity with thymidine and 2'-deoxycytidine | Drosophila melanogaster | 2'-deoxyguanosine 5'-phosphate + ADP | - |
? | |
2'-deoxyuridine + ATP | 90% of the activity with thymidine and 2'-deoxycytidine | Drosophila melanogaster | 2'-deoxyuridine 5'-phosphate + ADP | - |
? | |
3'-azidothymidine + ATP | 0.5% of the activity with thymidine and 2'-deoxycytidine | Drosophila melanogaster | 3'-azidothymidine 5'-phosphate + ADP | - |
? | |
3'-fluoro-2',3'-dideoxythymidine + ATP | 0.03% of the activity with thymidine and 2'-deoxycytidine | Drosophila melanogaster | 3'-fluoro-2',3'-dideoxythymidine 5'-phosphate + ADP | - |
? | |
5-fluoro-2'-deoxyuridine + ATP | activity is 10% higher than with thymidine and 2'-deoxycytidine | Drosophila melanogaster | 5-fluoro-2'-deoxyuridine 5'-phosphate + ADP | - |
? | |
additional information | no activity with acyclovir | Drosophila melanogaster | ? | - |
? | |
additional information | activity with CTP is 110% of the activity ATP | Drosophila melanogaster | ? | - |
? | |
additional information | no activity with dTMP | Drosophila melanogaster | ? | - |
? | |
additional information | no activity with acyclovir | Drosophila melanogaster S-2 | ? | - |
? | |
additional information | activity with CTP is 110% of the activity ATP | Drosophila melanogaster S-2 | ? | - |
? | |
additional information | no activity with dTMP | Drosophila melanogaster S-2 | ? | - |
? | |
thymidine + ATP | - |
Drosophila melanogaster | thymidine 5'-phosphate + ADP | - |
? | |
thymidine + ATP | - |
Drosophila melanogaster S-2 | thymidine 5'-phosphate + ADP | - |
? | |
thymidine + CTP | - |
Drosophila melanogaster | thymidine 5'-phosphate + CDP | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 30000, SDS-PAGE | Drosophila melanogaster |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 60 | - |
Drosophila melanogaster |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
15 | - |
thymidine | pH 8.0, 22°C | Drosophila melanogaster | |
15 | - |
2'-deoxycytidine | pH 8.0, 22°C | Drosophila melanogaster | |
18 | 19 | 2'-deoxyadenosine | pH 8.0, 22°C | Drosophila melanogaster | |
18 | 19 | 2'-deoxyguanosine | pH 8.0, 22°C | Drosophila melanogaster |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.001 | - |
thymidine | pH 8.0, 22°C, competitive with 2'-deoxycytidine | Drosophila melanogaster | |
0.001 | - |
2'-deoxycytidine | pH 8.0, 22°C, competitive with thymidine | Drosophila melanogaster | |
0.002 | - |
thymidine | pH 8.0, 22°C, reaction with 2'-deoxyadenosine | Drosophila melanogaster | |
0.003 | - |
thymidine | pH 8.0, 22°C, reaction with 2'-deoxyguanosine | Drosophila melanogaster | |
0.02 | - |
2'-deoxyadenosine | pH 8.0, 22°C, reaction with thymidine | Drosophila melanogaster | |
0.1 | - |
2'-deoxyguanosine | pH 8.0, 22°C, reaction with thymidine | Drosophila melanogaster | |
0.16 | - |
2'-deoxyadenosine | pH 8.0, 22°C, reaction with 2'-deoxyguanosine | Drosophila melanogaster | |
0.4 | - |
2'-deoxyguanosine | pH 8.0, 22°C, reaction with 2'-deoxyadenosine | Drosophila melanogaster |