Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme is induced by the calcium triggers interleukin-1beta and IgE/Ag due to calcium mobilization | Homo sapiens | |
Phorbol esters | e.g. PMA | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination, expression in HeLa and COS-1 cells, expression of GFP-tagged enzyme in HUVEC and COS-1 cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | siRNA to the gene encoding the enzyme for downregulation blocks arachidonic acid release and subsequent prostaglandin E2 production after stimulation | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
C2-ceramide | - |
Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.032 | - |
ATP | recombinant enzyme | Homo sapiens | |
0.187 | - |
ceramide | recombinant enzyme | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Homo sapiens | 5829 | - |
Golgi apparatus | - |
Homo sapiens | 5794 | - |
membrane | bound | Homo sapiens | 16020 | - |
additional information | subcellular localization may be dependent on the cell type | Homo sapiens | - |
- |
synaptic vesicle | - |
Homo sapiens | 8021 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | activation | Homo sapiens | |
Mg2+ | activation | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + ceramide | Homo sapiens | - |
ADP + ceramide 1-phosphate | ceramide 1-phosphate is a lipid signalling molecule involved in regulation of many physiological processes related to cell proliferation, apoptosis, and inflammation, most of them in concert with sphingosine 1-phosphate, ceramide 1-phosphate might act via extracellular cell surface receptors or intracellularly as second messenger | ? | |
additional information | Homo sapiens | the enzyme does not play a role in neutrophil priming, the enzyme regulates the activity and translocalization of cytosolic phospholipase isozyme A2alpha, overview, the enzyme is involved in prostaglandin biosynthesis, i.e. intracellular arachidonic acid production, which is blocked by enzyme gene silencing | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
lipoprotein | the enzyme is myristoylated at the N-terminus within the pleckstrin homology PH domain, required for activity | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Homo sapiens | - |
additional information | the enzyme is expressed in all or nearly all tissues and cell types | Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + C16-ceramide | lower activity | Homo sapiens | ADP + C16-ceramide 1-phosphate | - |
? | |
ATP + C18-ceramide | lower activity | Homo sapiens | ADP + C18-ceramide 1-phosphate | - |
? | |
ATP + C8-ceramide | best substrate | Homo sapiens | ADP + C8-ceramide 1-phosphate | - |
? | |
ATP + C8-dihydroceramide | high activity | Homo sapiens | ADP + C8-dihydroceramide 1-phosphate | - |
? | |
ATP + C8-phytoceramide | high activity | Homo sapiens | ADP + C8-phytoceramide 1-phosphate | - |
? | |
ATP + ceramide | - |
Homo sapiens | ADP + ceramide 1-phosphate | ceramide 1-phosphate is a lipid signalling molecule involved in regulation of many physiological processes related to cell proliferation, apoptosis, and inflammation, most of them in concert with sphingosine 1-phosphate, ceramide 1-phosphate might act via extracellular cell surface receptors or intracellularly as second messenger | ? | |
ATP + ceramide | the enzyme is specific for the cramide backbone, acylation of amino group in position 2 is required | Homo sapiens | ADP + ceramide 1-phosphate | - |
? | |
additional information | the enzyme does not play a role in neutrophil priming, the enzyme regulates the activity and translocalization of cytosolic phospholipase isozyme A2alpha, overview, the enzyme is involved in prostaglandin biosynthesis, i.e. intracellular arachidonic acid production, which is blocked by enzyme gene silencing | Homo sapiens | ? | - |
? | |
additional information | no activity with GTP, no activity with sphingosine and sphinganine, and with diacylglycerol | Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | the enzyme possesses an N-terminal pleckstrin homology PH domain, residues 32-121, and a C-terminal region which contains a Ca2+/calmodulin binding domain, residues 378-455, that flanks the catalytic domain, residues 124-433, the enzyme also contains 5 sequences stretches C1-C5 typical for lipid kinases | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
CERK | - |
Homo sapiens |
More | the enzyme belongs to the subfamily of diacylglyceride kinases DAGKs | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
sharp optimum | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | GGDG motif binding site is located in the C2-subdomain | Homo sapiens | |
additional information | no activity with GTP | Homo sapiens |