Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant enzymes in strains TG1 and N43 | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
H198A | site-directed mutagenesis, 50% reduction of the minimal inhibitory concentration MIC level of oleandomycin and 50% reduced activity compared to the wild-type enzyme | Escherichia coli |
H205A | site-directed mutagenesis, 87.5% reduction of the minimal inhibitory concentration MIC level of oleandomycin and 50% reduced activity compared to the wild-type enzyme | Escherichia coli |
H205N | site-directed mutagenesis, unaffected minimal inhibitory concentration MIC level of oleandomycin, but more than 99% reduced activity compared to the wild-type enzyme | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
oleandomycin | - |
Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
KCl | - |
Escherichia coli | |
Mg2+ | - |
Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
gene mphB | - |
Escherichia coli CU1 | - |
gene mphB | - |
Purification (Comment) | Organism |
---|---|
recombinant wild-type and mutant enzymes from strain TG1 | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
substrate specificities of the mutant enzymes | Escherichia coli |
0.0007 | - |
purified recombinant mutant H205A, substrate oleandomycin | Escherichia coli |
0.04 | - |
purified recombinant wild-type enzyme, substrate roxithromycin or rokitamycin | Escherichia coli |
0.05 | - |
purified recombinant wild-type enzyme, substrate josamycin or tylosin | Escherichia coli |
0.052 | - |
purified recombinant mutant H198A, substrate oleandomycin | Escherichia coli |
0.056 | - |
purified recombinant mutant H205N, substrate oleandomycin | Escherichia coli |
0.06 | - |
purified recombinant wild-type enzyme, substrate troleandomycin, clarithromycin, azithromycin, or erythromycin | Escherichia coli |
0.1 | - |
purified recombinant wild-type enzyme, substrate oleandomycin | Escherichia coli |
0.16 | - |
purified recombinant wild-type enzyme, substrate kitasamycin | Escherichia coli |
0.21 | - |
purified recombinant wild-type enzyme, substrate spiramycin | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + azithromycin | 15-membered ring macrolide | Escherichia coli | ADP + azithromycin 2'-O-phosphate | - |
? | |
ATP + azithromycin | 15-membered ring macrolide | Escherichia coli CU1 | ADP + azithromycin 2'-O-phosphate | - |
? | |
ATP + clarithromycin | 14-membered ring macrolide | Escherichia coli | ADP + clarithromycin 2'-O-phosphate | - |
? | |
ATP + clarithromycin | 14-membered ring macrolide | Escherichia coli CU1 | ADP + clarithromycin 2'-O-phosphate | - |
? | |
ATP + erythromycin | 14-membered ring macrolide | Escherichia coli | ADP + erythromycin 2'-O-phosphate | - |
? | |
ATP + josamycin | 15-membered ring macrolide | Escherichia coli | ADP + josamycin 2'-O-phosphate | - |
? | |
ATP + kitasamycin | 15-membered ring macrolide | Escherichia coli | ADP + kitasamycin 2'-O-phosphate | - |
? | |
ATP + oleandomycin | 14-membered ring macrolide | Escherichia coli | ADP + oleandomycin 2'-O-phosphate | - |
? | |
ATP + oleandomycin | 14-membered ring macrolide | Escherichia coli CU1 | ADP + oleandomycin 2'-O-phosphate | - |
? | |
ATP + rokitamycin | 15-membered ring macrolide | Escherichia coli | ADP + rokitamycin 2'-O-phosphate | - |
? | |
ATP + roxithromycin | 14-membered ring macrolide | Escherichia coli | ADP + roxithromycin 2'-O-phosphate | - |
? | |
ATP + spiramycin | 15-membered ring macrolide | Escherichia coli | ADP + spiramycin 2'-O-phosphate | - |
? | |
ATP + spiramycin | 15-membered ring macrolide | Escherichia coli CU1 | ADP + spiramycin 2'-O-phosphate | - |
? | |
ATP + troleandomycin + H2O | 14-membered ring macrolide | Escherichia coli | ADP + troleandomycin 2'-O-phosphate + acetate | - |
? | |
ATP + tylosin | 15-membered ring macrolide | Escherichia coli | ADP + tylosin 2'-O-phosphate | - |
? | |
additional information | substrate specificities of wild-type and mutant enzymes, structure-function considerations | Escherichia coli | ? | - |
? | |
additional information | substrate specificities of wild-type and mutant enzymes, structure-function considerations | Escherichia coli CU1 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
macrolide 2'-phosphotransferase II | - |
Escherichia coli |
MPH(2') | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
assay at | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Escherichia coli |