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Literature summary for 2.7.1.134 extracted from
- Properties of the inositol 3,4,5,6-tetrakisphosphate 1-kinase purified from rat liver. Regulation of enzyme activity by inositol 1,3,4-trisphosphate (1997), J. Biol. Chem., 272, 2285-2290.
General Stability
| General Stability | Organism |
|---|---|
| 10 mM sodium diphosphate stabilizes ammonium sulfate precipitated enzyme, no loss of activity after 2 days at 4°C | Rattus norvegicus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| inositol 1,3,4-trisphosphate | competitive inhibition | Rattus norvegicus |  |
| inositol-1,3,4,5,6-pentakisphosphate | competitive inhibition | Rattus norvegicus | |
| Inositol-1,3,4,5-tetrakisphosphate | competitive inhibition | Rattus norvegicus | |
| Inositol-1,3,4,6-tetrakisphosphate | competitive inhibition | Rattus norvegicus | |
| inositol-1,4,5-trisphosphate | competitive inhibition | Rattus norvegicus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00036 | - |
myo-inositol-3,4,5,6-tetrakisphosphate | pH 7.2, 37°C | Rattus norvegicus | |
| 0.01 | - |
ATP | pH 7.2, 37°C | Rattus norvegicus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| soluble | - |
Rattus norvegicus | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | activation at 0.0001 mM, 10-20% inhibition at 0.001 mM Ca2+ | Rattus norvegicus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 46000 | - |
liver enzyme, gel filtration | Rattus norvegicus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| heparin-agarose, phenyl-Sepharose, Mono Q Matrex Blue A | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Rattus norvegicus | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -70°C, 1 mg/ml bovine serum albumin, 20% glycerol, 6 months, no loss of activity | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 1D-myo-inositol 3,4,5,6-tetrakisphosphate | - |
Rattus norvegicus | ADP + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate | - |
? | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0002 | - |
inositol 1,3,4-trisphosphate | pH 7.2, 37°C | Rattus norvegicus | |
| 0.009 | - |
Inositol 1,3,4,5-tetrakisphosphate | pH 7.2, 37°C | Rattus norvegicus | |
| 0.015 | - |
Inositol 1,3,4,5,6-pentakisphosphate | pH 7.2, 37°C | Rattus norvegicus | |
| 0.015 | - |
Inositol 1,3,4,6-tetrakisphosphate | pH 7.2, 37°C | Rattus norvegicus | |
| 0.048 | - |
Inositol 1,4,5-trisphosphate | pH 7.2, 37°C | Rattus norvegicus | |
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