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Literature summary for 2.7.1.134 extracted from

  • Tan, Z.; Bruzik, K.S.; Shears, S.B.
    Properties of the inositol 3,4,5,6-tetrakisphosphate 1-kinase purified from rat liver. Regulation of enzyme activity by inositol 1,3,4-trisphosphate (1997), J. Biol. Chem., 272, 2285-2290.
    View publication on PubMed
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General Stability

General Stability Organism
10 mM sodium diphosphate stabilizes ammonium sulfate precipitated enzyme, no loss of activity after 2 days at 4°C Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
inositol 1,3,4-trisphosphate competitive inhibition Rattus norvegicus
inositol-1,3,4,5,6-pentakisphosphate competitive inhibition Rattus norvegicus
Inositol-1,3,4,5-tetrakisphosphate competitive inhibition Rattus norvegicus
Inositol-1,3,4,6-tetrakisphosphate competitive inhibition Rattus norvegicus
inositol-1,4,5-trisphosphate competitive inhibition Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00036
-
 myo-inositol-3,4,5,6-tetrakisphosphate pH 7.2, 37°C Rattus norvegicus
0.01
-
 ATP pH 7.2, 37°C Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
 Rattus norvegicus
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activation at 0.0001 mM, 10-20% inhibition at 0.001 mM Ca2+ Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
46000
-
 liver enzyme, gel filtration Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
heparin-agarose, phenyl-Sepharose, Mono Q Matrex Blue A Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Rattus norvegicus
-

Storage Stability

Storage Stability Organism
-70°C, 1 mg/ml bovine serum albumin, 20% glycerol, 6 months, no loss of activity Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 1D-myo-inositol 3,4,5,6-tetrakisphosphate
-
 Rattus norvegicus ADP + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate
-
 ?

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0002
-
 inositol 1,3,4-trisphosphate pH 7.2, 37°C Rattus norvegicus
0.009
-
 Inositol 1,3,4,5-tetrakisphosphate pH 7.2, 37°C Rattus norvegicus
0.015
-
 Inositol 1,3,4,6-tetrakisphosphate pH 7.2, 37°C Rattus norvegicus
0.015
-
 Inositol 1,3,4,5,6-pentakisphosphate pH 7.2, 37°C Rattus norvegicus
0.048
-
 Inositol 1,4,5-trisphosphate pH 7.2, 37°C Rattus norvegicus