BRENDA - Enzyme Database show
show all sequences of 2.7.1.130

Lipid A 4-kinase from Escherichia coli: enzyme assay and preparation of 4-32P-labeled probes of high specific radioactivity

Hampton, R.Y.; Raetz, C.R.H.; Methods Enzymol. 209, 466-475 (1992)

Data extracted from this reference:

Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
probably a membrane protein
Escherichia coli
16020
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
required
Escherichia coli
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Escherichia coli
-
-
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
assay procedure
Escherichia coli
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + 2,3-bis(3-hydroxytetradecanoyl)-D-glucosaminyl-(beta-D-1,6-)-2,3-bis(3-hydroxytetradecanoyl)-D-glucosaminyl beta-phosphate
no activity with lipid X
640806
Escherichia coli
ADP + 2,3,2',3'-tetrakis(3-hydroxytetradecanoyl)-D-glucosaminyl-1,6-beta-D-glucosamine 1,4'-bisphosphate
-
640806
Escherichia coli
-
ATP + 2,3-bis[3-(tetradecanoyl)tetradecanoyl]-D-glucosaminyl-(beta-D-1,6-)-2,3-bis(3-hydroxytetradecanoyl)-D-glucosaminyl beta-phosphate
-
640806
Escherichia coli
ADP + 2,3,2',3'-tetrakis[3-(tetradecanoyl)tetradecanoyl]-D-glucosaminyl-1,6-beta-D-glucosamine 1,4'-bisphosphate
-
640806
Escherichia coli
?
ATP + 2-(3-hydroxytetradecanoyl)-D-glucosaminyl-(beta-D-1,6-)-2,3-bis(3-hydroxytetradecanoyl)-D-glucosaminyl beta-phosphate
-
640806
Escherichia coli
ADP + 2,2',3'-tris(3-hydroxytetradecanoyl)-D-glucosaminyl-1,6-beta-D-glucosamine 1,4'-bisphosphate
-
640806
Escherichia coli
?
ATP + 2-(3-hydroxytetradecanoyl)-D-glucosaminyl-(beta-D-1,6-)-2-(3-hydroxytetradecanoyl)-D-glucosaminyl beta-phosphate
-
640806
Escherichia coli
ADP + 2,2'-bis(3-hydroxytetradecanoyl)-D-glucosaminyl-1,6-beta-D-glucosamine 1,4'-bisphosphate
-
640806
Escherichia coli
?
ATP + 3-aza-2,3-bis(3-hydroxytetradecanoyl)-D-glucosaminyl-(beta-D-1,6-)-2,3-bis(3-hydroxytetradecanoyl)-D-glucosaminyl beta-phosphate
-
640806
Escherichia coli
ADP + 3-aza-2,3,2',3'-tetrakis(3-hydroxytetradecanoyl)-D-glucosaminyl-1,6-beta-D-glucosamine 1,4'-bisphosphate
-
640806
Escherichia coli
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Escherichia coli
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.4
-
assay at
Escherichia coli
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
probably a membrane protein
Escherichia coli
16020
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
required
Escherichia coli
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
assay procedure
Escherichia coli
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + 2,3-bis(3-hydroxytetradecanoyl)-D-glucosaminyl-(beta-D-1,6-)-2,3-bis(3-hydroxytetradecanoyl)-D-glucosaminyl beta-phosphate
no activity with lipid X
640806
Escherichia coli
ADP + 2,3,2',3'-tetrakis(3-hydroxytetradecanoyl)-D-glucosaminyl-1,6-beta-D-glucosamine 1,4'-bisphosphate
-
640806
Escherichia coli
-
ATP + 2,3-bis[3-(tetradecanoyl)tetradecanoyl]-D-glucosaminyl-(beta-D-1,6-)-2,3-bis(3-hydroxytetradecanoyl)-D-glucosaminyl beta-phosphate
-
640806
Escherichia coli
ADP + 2,3,2',3'-tetrakis[3-(tetradecanoyl)tetradecanoyl]-D-glucosaminyl-1,6-beta-D-glucosamine 1,4'-bisphosphate
-
640806
Escherichia coli
?
ATP + 2-(3-hydroxytetradecanoyl)-D-glucosaminyl-(beta-D-1,6-)-2,3-bis(3-hydroxytetradecanoyl)-D-glucosaminyl beta-phosphate
-
640806
Escherichia coli
ADP + 2,2',3'-tris(3-hydroxytetradecanoyl)-D-glucosaminyl-1,6-beta-D-glucosamine 1,4'-bisphosphate
-
640806
Escherichia coli
?
ATP + 2-(3-hydroxytetradecanoyl)-D-glucosaminyl-(beta-D-1,6-)-2-(3-hydroxytetradecanoyl)-D-glucosaminyl beta-phosphate
-
640806
Escherichia coli
ADP + 2,2'-bis(3-hydroxytetradecanoyl)-D-glucosaminyl-1,6-beta-D-glucosamine 1,4'-bisphosphate
-
640806
Escherichia coli
?
ATP + 3-aza-2,3-bis(3-hydroxytetradecanoyl)-D-glucosaminyl-(beta-D-1,6-)-2,3-bis(3-hydroxytetradecanoyl)-D-glucosaminyl beta-phosphate
-
640806
Escherichia coli
ADP + 3-aza-2,3,2',3'-tetrakis(3-hydroxytetradecanoyl)-D-glucosaminyl-1,6-beta-D-glucosamine 1,4'-bisphosphate
-
640806
Escherichia coli
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Escherichia coli
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.4
-
assay at
Escherichia coli
Other publictions for EC 2.7.1.130
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
738621
Emptage
Structural basis of lipid bind ...
Aquifex aeolicus
J. Biol. Chem.
289
24059-24068
2014
-
1
1
1
15
-
-
12
1
-
-
1
-
1
-
-
1
-
-
-
-
-
2
-
-
-
-
15
-
-
-
-
-
-
-
-
1
1
-
1
15
-
-
-
-
12
1
-
-
1
-
-
-
1
-
-
-
-
2
-
-
-
-
15
-
-
-
-
-
2
2
-
-
-
737675
Emptage
Mechanistic characterization o ...
Aquifex aeolicus
Biochemistry
52
2280-2290
2013
-
-
1
1
17
-
5
19
1
4
-
1
-
1
-
-
1
1
-
-
-
-
2
-
1
-
-
15
3
-
-
1
-
-
-
-
-
1
1
1
17
-
-
5
-
19
1
4
-
1
-
-
-
1
-
-
-
-
2
-
1
-
-
15
3
-
-
-
-
4
4
-
-
-
723650
Emptage
Crystal structure of LpxK, the ...
Aquifex aeolicus
Proc. Natl. Acad. Sci. USA
109
12956-12961
2012
-
-
-
1
8
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
640807
Garrett
Identification of the gene enc ...
Escherichia coli
J. Biol. Chem.
272
21855-21864
1997
-
-
1
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
640806
Hampton
Lipid A 4-kinase from Escheric ...
Escherichia coli
Methods Enzymol.
209
466-475
1992
-
-
-
-
-
-
-
-
1
1
-
-
-
2
-
-
-
-
-
-
1
-
5
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
1
-
5
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
640805
Ray
The biosynthesis of gram-negat ...
Escherichia coli
J. Biol. Chem.
262
1122-1128
1987
1
-
-
-
-
2
2
-
1
1
-
1
-
3
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
2
-
2
-
-
1
1
-
1
-
-
-
-
-
-
-
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5
-
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