BRENDA - Enzyme Database show
show all sequences of 2.7.1.130

The biosynthesis of gram-negative endotoxin. A novel kinase in Escherichia coli membranes that incorporates the 4-phosphate of lipid A

Ray, B.L.; Raetz, C.R.H.; J. Biol. Chem. 262, 1122-1128 (1987)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
cardiolipin
other phospholipids including phosphatidylethanolamine, phosphatidylserine, phosphatidylcholine and phosphatidylglycerol are not as effective as cardiolipin
Escherichia coli
General Stability
General Stability
Organism
25% glycerol stabilizes
Escherichia coli
phosphoenolpyruvate stabilizes
Escherichia coli
Inhibitors
Inhibitors
Commentary
Organism
Structure
Nonidet P-40
inactivation by preincubation
Escherichia coli
octylglucoside
inactivation by preincubation
Escherichia coli
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
-
Escherichia coli
16020
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
required
Escherichia coli
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ATP + 2,3-bis(3-hydroxytetradecanoyl)-D-glucosaminyl-(beta-D-1,6-)-2,3-bis(3-hydroxytetradecanoyl)-D-glucosaminyl beta-phosphate
Escherichia coli
involved with EC 2.3.1.129 and 2.4.1.182 in the biosynthesis of the phosphorylated glycolipid, lipid A, in the outer membrane
ADP + 2,3,2',3'-tetrakis(3-hydroxytetradecanoyl)-D-glucosaminyl-1,6-beta-D-glucosamine 1,4'-bisphosphate
-
Escherichia coli
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Escherichia coli
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + 2,3-bis(3-hydroxytetradecanoyl)-D-glucosaminyl-(beta-D-1,6-)-2,3-bis(3-hydroxytetradecanoyl)-D-glucosaminyl beta-phosphate
-
640805
Escherichia coli
ADP + 2,3,2',3'-tetrakis(3-hydroxytetradecanoyl)-D-glucosaminyl-1,6-beta-D-glucosamine 1,4'-bisphosphate
-
640805
Escherichia coli
?
ATP + 2,3-bis(3-hydroxytetradecanoyl)-D-glucosaminyl-(beta-D-1,6-)-2,3-bis(3-hydroxytetradecanoyl)-D-glucosaminyl beta-phosphate
involved with EC 2.3.1.129 and 2.4.1.182 in the biosynthesis of the phosphorylated glycolipid, lipid A, in the outer membrane
640805
Escherichia coli
ADP + 2,3,2',3'-tetrakis(3-hydroxytetradecanoyl)-D-glucosaminyl-1,6-beta-D-glucosamine 1,4'-bisphosphate
-
640805
Escherichia coli
?
CTP + 2,3-bis(3-hydroxytetradecanoyl)-D-glucosaminyl-(beta-D-1,6-)-2,3-bis(3-hydroxytetradecanoyl)-D-glucosaminyl beta-phosphate
approx. 50% of activity with ATP
640805
Escherichia coli
CDP + 2,3,2',3'-tetrakis(3-hydroxytetradecanoyl)-D-glucosaminyl-1,6-beta-D-glucosamine 1,4'-bisphosphate
-
640805
Escherichia coli
?
GTP + 2,3-bis(3-hydroxytetradecanoyl)-D-glucosaminyl-(beta-D-1,6-)-2,3-bis(3-hydroxytetradecanoyl)-D-glucosaminyl beta-phosphate
approx. 50% of activity with ATP
640805
Escherichia coli
GDP + 2,3,2',3'-tetrakis(3-hydroxytetradecanoyl)-D-glucosaminyl-1,6-beta-D-glucosamine 1,4'-bisphosphate
-
640805
Escherichia coli
?
UTP + 2,3-bis(3-hydroxytetradecanoyl)-D-glucosaminyl-(beta-D-1,6-)-2,3-bis(3-hydroxytetradecanoyl)-D-glucosaminyl beta-phosphate
approx. 50% of activity with ATP
640805
Escherichia coli
UDP + 2,3,2',3'-tetrakis(3-hydroxytetradecanoyl)-D-glucosaminyl-1,6-beta-D-glucosamine 1,4'-bisphosphate
-
640805
Escherichia coli
?
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
cardiolipin
other phospholipids including phosphatidylethanolamine, phosphatidylserine, phosphatidylcholine and phosphatidylglycerol are not as effective as cardiolipin
Escherichia coli
General Stability (protein specific)
General Stability
Organism
25% glycerol stabilizes
Escherichia coli
phosphoenolpyruvate stabilizes
Escherichia coli
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Nonidet P-40
inactivation by preincubation
Escherichia coli
octylglucoside
inactivation by preincubation
Escherichia coli
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
-
Escherichia coli
16020
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
required
Escherichia coli
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ATP + 2,3-bis(3-hydroxytetradecanoyl)-D-glucosaminyl-(beta-D-1,6-)-2,3-bis(3-hydroxytetradecanoyl)-D-glucosaminyl beta-phosphate
Escherichia coli
involved with EC 2.3.1.129 and 2.4.1.182 in the biosynthesis of the phosphorylated glycolipid, lipid A, in the outer membrane
ADP + 2,3,2',3'-tetrakis(3-hydroxytetradecanoyl)-D-glucosaminyl-1,6-beta-D-glucosamine 1,4'-bisphosphate
-
Escherichia coli
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + 2,3-bis(3-hydroxytetradecanoyl)-D-glucosaminyl-(beta-D-1,6-)-2,3-bis(3-hydroxytetradecanoyl)-D-glucosaminyl beta-phosphate
-
640805
Escherichia coli
ADP + 2,3,2',3'-tetrakis(3-hydroxytetradecanoyl)-D-glucosaminyl-1,6-beta-D-glucosamine 1,4'-bisphosphate
-
640805
Escherichia coli
?
ATP + 2,3-bis(3-hydroxytetradecanoyl)-D-glucosaminyl-(beta-D-1,6-)-2,3-bis(3-hydroxytetradecanoyl)-D-glucosaminyl beta-phosphate
involved with EC 2.3.1.129 and 2.4.1.182 in the biosynthesis of the phosphorylated glycolipid, lipid A, in the outer membrane
640805
Escherichia coli
ADP + 2,3,2',3'-tetrakis(3-hydroxytetradecanoyl)-D-glucosaminyl-1,6-beta-D-glucosamine 1,4'-bisphosphate
-
640805
Escherichia coli
?
CTP + 2,3-bis(3-hydroxytetradecanoyl)-D-glucosaminyl-(beta-D-1,6-)-2,3-bis(3-hydroxytetradecanoyl)-D-glucosaminyl beta-phosphate
approx. 50% of activity with ATP
640805
Escherichia coli
CDP + 2,3,2',3'-tetrakis(3-hydroxytetradecanoyl)-D-glucosaminyl-1,6-beta-D-glucosamine 1,4'-bisphosphate
-
640805
Escherichia coli
?
GTP + 2,3-bis(3-hydroxytetradecanoyl)-D-glucosaminyl-(beta-D-1,6-)-2,3-bis(3-hydroxytetradecanoyl)-D-glucosaminyl beta-phosphate
approx. 50% of activity with ATP
640805
Escherichia coli
GDP + 2,3,2',3'-tetrakis(3-hydroxytetradecanoyl)-D-glucosaminyl-1,6-beta-D-glucosamine 1,4'-bisphosphate
-
640805
Escherichia coli
?
UTP + 2,3-bis(3-hydroxytetradecanoyl)-D-glucosaminyl-(beta-D-1,6-)-2,3-bis(3-hydroxytetradecanoyl)-D-glucosaminyl beta-phosphate
approx. 50% of activity with ATP
640805
Escherichia coli
UDP + 2,3,2',3'-tetrakis(3-hydroxytetradecanoyl)-D-glucosaminyl-1,6-beta-D-glucosamine 1,4'-bisphosphate
-
640805
Escherichia coli
?
Other publictions for EC 2.7.1.130
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
738621
Emptage
Structural basis of lipid bind ...
Aquifex aeolicus
J. Biol. Chem.
289
24059-24068
2014
-
1
1
1
15
-
-
12
1
-
-
1
-
1
-
-
1
-
-
-
-
-
2
-
-
-
-
15
-
-
-
-
-
-
-
-
1
1
-
1
15
-
-
-
-
12
1
-
-
1
-
-
-
1
-
-
-
-
2
-
-
-
-
15
-
-
-
-
-
2
2
-
-
-
737675
Emptage
Mechanistic characterization o ...
Aquifex aeolicus
Biochemistry
52
2280-2290
2013
-
-
1
1
17
-
5
19
1
4
-
1
-
1
-
-
1
1
-
-
-
-
2
-
1
-
-
15
3
-
-
1
-
-
-
-
-
1
1
1
17
-
-
5
-
19
1
4
-
1
-
-
-
1
-
-
-
-
2
-
1
-
-
15
3
-
-
-
-
4
4
-
-
-
723650
Emptage
Crystal structure of LpxK, the ...
Aquifex aeolicus
Proc. Natl. Acad. Sci. USA
109
12956-12961
2012
-
-
-
1
8
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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-
-
1
8
-
-
-
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-
-
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-
-
-
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
640807
Garrett
Identification of the gene enc ...
Escherichia coli
J. Biol. Chem.
272
21855-21864
1997
-
-
1
-
-
-
-
-
-
-
-
-
-
3
-
-
-
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-
-
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1
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-
-
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-
-
-
-
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-
-
-
-
-
-
-
-
-
-
640806
Hampton
Lipid A 4-kinase from Escheric ...
Escherichia coli
Methods Enzymol.
209
466-475
1992
-
-
-
-
-
-
-
-
1
1
-
-
-
2
-
-
-
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1
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5
-
1
-
-
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1
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-
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1
1
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-
-
-
1
-
5
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
640805
Ray
The biosynthesis of gram-negat ...
Escherichia coli
J. Biol. Chem.
262
1122-1128
1987
1
-
-
-
-
2
2
-
1
1
-
1
-
3
-
-
-
-
-
-
-
-
5
-
-
-
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-
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-
-
-
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-
1
-
-
-
-
-
2
-
2
-
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1
1
-
1
-
-
-
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-
-
-
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5
-
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