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Literature summary for 2.7.1.127 extracted from

  • Wei, R.; Cao, G.; Deng, Z.; Su, J.; Cai, L.
    miR-140-5p attenuates chemotherapeutic drug-induced cell death by regulating autophagy through inositol 1,4,5-trisphosphate kinase 2 (IP3k2) in human osteosarcoma cells (2016), Biosci. Rep., 36, art:e00392 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.1.127

Cloned(Commentary)

Cloned (Comment) Organism
gene IP3k2, quantitative real-time PCR expression analysis Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information IP3k2 knockdown by siRNA Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + 1D-myo-inositol 1,4,5-trisphosphate Homo sapiens
-
 ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens P27987
-
-

Source Tissue

Source Tissue Comment Organism Textmining
osteosarcoma cell
-
 Homo sapiens
-
SAOS-2 cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 1D-myo-inositol 1,4,5-trisphosphate
-
 Homo sapiens ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate
-
 ?

Synonyms

Synonyms Comment Organism
inositol 1,4,5-trisphosphate kinase 2
-
 Homo sapiens
inositol-trisphosphate 3-kinase B UniProt Homo sapiens
IP3K2
-
 Homo sapiens
Itpkb
-
 Homo sapiens

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Homo sapiens

Expression

Organism Comment Expression
Homo sapiens the microRNA miR-140-5p inhibits IP3k2 expression in osteosarcoma cells via predicted 3'-UTR target sites and mRNA destabilization. Inhibiting IP3k2 protein expression promotes autophagy in response to anticancer drug treatment down
Homo sapiens IP3K2 expression and Ca2+ entry are upregulated in osteosarcoma cells following treatment with chemotherapeutic drugs, e.g. doxorubicin and cisplatin up

General Information

General Information Comment Organism
malfunction the expression of microRNA-140, termed miR-140-5p, is highly induced during chemotherapy of osteosarcoma cells accompanied by upregulated autophagy. The increased miR-140-5p expression levels upregulate anticancer drug-induced autophagy in osteosarcoma cells and ameliorate the anticancer drug-induced cell proliferation and viability decrease. miR-140-5p regulates this context-specific autophagy through its target, inositol 1,4,5-trisphosphate kinase 2 (IP3k2). miR-140-5p mediated drug-resistance in osteosarcoma cells occurs by inducing autophagy, and miRNA regulation of autophagy through modulation of IP3 signalling. IP3K2 expression and Ca2+ entry are upregulated in osteosarcoma cells following treatment with chemotherapeutic drugs Homo sapiens