Cloned (Comment) | Organism |
---|---|
gene ITPKA, recombinnant expression of N-terminally GST-tagged isozyme ITPKA in Escherichia coli strain BL21 C41lys | Mus musculus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
synapse | spine | Mus musculus | 45202 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | activates | Mus musculus | |
Mg2+ | required | Mus musculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 1D-myo-inositol 1,4,5-trisphosphate | Mus musculus | - |
ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate | - |
? | |
ATP + 1D-myo-inositol 1,4,5-trisphosphate | Mus musculus C57BL/6 | - |
ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | Q8R071 | gene Itpka | - |
Mus musculus C57BL/6 | Q8R071 | gene Itpka | - |
Purification (Comment) | Organism |
---|---|
recombinant N-terminally GST-tagged isozyme ITPKA from Escherichia coli strain BL21 C41lys by glutathione affinity chromatography | Mus musculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cervical spine | - |
Mus musculus | - |
hippocampus | - |
Mus musculus | - |
neuron | isozyme inositol-1,4,5-trisphosphate-3-kinase-A is a neuron-specific, actin bundling protein concentrated at dendritic spines | Mus musculus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 1D-myo-inositol 1,4,5-trisphosphate | - |
Mus musculus | ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate | - |
? | |
ATP + 1D-myo-inositol 1,4,5-trisphosphate | - |
Mus musculus C57BL/6 | ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
inositol-1,4,5-trisphosphate-3-kinase-A | - |
Mus musculus |
ITPKA | - |
Mus musculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Mus musculus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Mus musculus |
General Information | Comment | Organism |
---|---|---|
malfunction | ITPKA depletion in mice increases the number of hippocampal spine-synapses while reducing average spine length, depletion of ITPKA reduces the length of dendritic spines of hippocampal pyramidal cells | Mus musculus |
physiological function | in addition to cross-linking actin filaments, ITPKA strongly inhibits Arp2/3-complex induced actin filament branching by displacing the complex from F-actin. n vivo ITPKA negatively regulates formation and/or maintenance of synaptic contacts in the mammalian brain. On the molecular level this effect appears to result from the ITPKA-mediated inhibition of Arp2/3-complex F-actin branching activity. ITPKA does not affect the F-actin bundling activity of drebrin A | Mus musculus |