Literature summary for 2.7.1.127 extracted from

Hascakova-Bartova, R.; Pouillon, V.; Dewaste, V.; Moreau, C.; Jacques, C.; Banting, G.; Schurmans, S.; Erneux, C.
Identification and subcellular distribution of endogenous Ins(1,4,5)P(3) 3-kinase B in mouse tissues (2004), Biochem. Biophys. Res. Commun., 323, 920-925.

Search for more literature for 2.7.1.127

Cloned(Commentary)

Cloned (Comment)	Organism
expression of isozymes IP3-3KA, IP3-3KB, and IP3-3KC in COS-7 cells	Mus musculus

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
particle-bound	probably endoplasmic reticulum	Mus musculus	-	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Ca2+	at 0.01 mM	Mus musculus
Mg2+	-	Mus musculus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
48000	-	x * 53000, recombinant isozyme IP3-3KA, SDS-PAGE, x * 98000, recombinant isozyme IP3-3KC, SDS-PAGE, x * 125000, recombinant isozyme IP3-3KB, SDS-PAGE, x * 48000, native brain isozyme IP3-3KA, SDS-PAGE, x * 110000-120000, native isozyme IP3-3KB, tissue-dependent, SDS-PAGE	Mus musculus
53000	-	x * 53000, recombinant isozyme IP3-3KA, SDS-PAGE, x * 98000, recombinant isozyme IP3-3KC, SDS-PAGE, x * 125000, recombinant isozyme IP3-3KB, SDS-PAGE, x * 48000, native brain isozyme IP3-3KA, SDS-PAGE, x * 110000-120000, native isozyme IP3-3KB, tissue-dependent, SDS-PAGE	Mus musculus
98000	-	x * 53000, recombinant isozyme IP3-3KA, SDS-PAGE, x * 98000, recombinant isozyme IP3-3KC, SDS-PAGE, x * 125000, recombinant isozyme IP3-3KB, SDS-PAGE, x * 48000, native brain isozyme IP3-3KA, SDS-PAGE, x * 110000-120000, native isozyme IP3-3KB, tissue-dependent, SDS-PAGE	Mus musculus
125000	-	x * 53000, recombinant isozyme IP3-3KA, SDS-PAGE, x * 98000, recombinant isozyme IP3-3KC, SDS-PAGE, x * 125000, recombinant isozyme IP3-3KB, SDS-PAGE, x * 48000, native brain isozyme IP3-3KA, SDS-PAGE, x * 110000-120000, native isozyme IP3-3KB, tissue-dependent, SDS-PAGE	Mus musculus

Organism

Organism	UniProt	Comment	Textmining
Mus musculus	-	wild-type strain C57BL/6 and enzyme-deficient strain, 3 isozymes IP3-3KA, IP3-3KB, and IP3-3KC	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
proteolytic modification	isozyme IP3-3KB is sensitive to proteolysis at the N-terminus generating active fragments of lower molecular mass	Mus musculus

Source Tissue

Source Tissue	Comment	Organism	Textmining
brain	higher expression of isozyme IP3-3KB	Mus musculus	-
lung	higher expression of isozyme IP3-3KB	Mus musculus	-
additional information	tissue-dependent expression of isozyme IP3-3KB	Mus musculus	-
thymus	higher expression of isozyme IP3-3KB	Mus musculus	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.00044	-	mouse brain second particulate fraction	Mus musculus
0.0026	-	recombinant enzyme in the first particulate fraction of transfected COS-7 cells	Mus musculus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + 1D-myo-inositol 1,4,5-trisphosphate	-	Mus musculus	ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate	-	?

Subunits

Subunits	Comment	Organism
?	x * 53000, recombinant isozyme IP3-3KA, SDS-PAGE, x * 98000, recombinant isozyme IP3-3KC, SDS-PAGE, x * 125000, recombinant isozyme IP3-3KB, SDS-PAGE, x * 48000, native brain isozyme IP3-3KA, SDS-PAGE, x * 110000-120000, native isozyme IP3-3KB, tissue-dependent, SDS-PAGE	Mus musculus

Synonyms

Synonyms	Comment	Organism
inositol 1,4,5-trisphosphate 3-kinase	-	Mus musculus
Ins(1,4,5)P(3) 3-kinase B	-	Mus musculus
IP3-3K	-	Mus musculus

Cofactor

Cofactor	Comment	Organism	Structure
ATP	-	Mus musculus

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)