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Literature summary for 2.7.1.127 extracted from
- Rat inositol 1,4,5-trisphosphate 3-kinase C is enzymatically specialized for basal cellular inositol trisphosphate phosphorylation and shuttles actively between nucleus and cytoplasm (2003), J. Biol. Chem., 278, 19765-19776.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 1D-myo-inositol 1,3,4,5-tetrakisphosphate | recombinant, catalytically active fragment of isoform C, allosteric product activation in the absence of Ca2+/calmodulin, which per se activates enzyme and abolishes the allosteric effect | Rattus norvegicus |  |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| cloning of a full-length cDNA encoding IP3K isoform C from tongue epithelium, cloning of an enzymatically active and Ca2+/calmodulin-regulated fragment of isoform C and expression in Escherichia coli BL21(DE3)pRIL | Rattus norvegicus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1D-myo-inositol 1,3,4,5-tetrakisphosphate | marked product inhibition, isoforms A and B | Rattus norvegicus | |
| 1D-myo-inositol 1,4,5-trisphosphate | recombinant, catalytically active fragment of isoform C, substrate inhibition by high concentrations | Rattus norvegicus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0002 | - |
1D-myo-inositol 1,4,5-trisphosphate | pH 7.5, 30°C, recombinant, catalytically active fragment of isoform C | Rattus norvegicus | |
| 0.0015 | - |
1D-myo-inositol 2,4,5-trisphosphate | pH 7.5, 30°C, in absence of Ca2+/calmodulin, recombinant, catalytically active fragment of isoform C | Rattus norvegicus | |
| 0.005 | - |
1D-myo-inositol 2,4,5-trisphosphate | pH 7.5, 30°C, in presence of 100 nM Ca2+/calmodulin, recombinant, catalytically active fragment of isoform C | Rattus norvegicus | |
| 0.033 | - |
ATP | pH 7.5, 30°C, in absence of Ca2+/calmodulin, recombinant, catalytically active fragment of isoform C | Rattus norvegicus | |
| 0.052 | - |
ATP | pH 7.5, 30°C, in presence of 100 nM Ca2+/calmodulin, recombinant, catalytically active fragment of isoform C | Rattus norvegicus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | isoform C undergoes active nucleocytoplasmic shuttling | Rattus norvegicus | 5737 | - |
| nucleus | isoform C is actively transported into and out of the nucleus, a leucine-rich nuclear export signal and a nuclear import activity are localized in the N-terminal domain | Rattus norvegicus | 5634 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | isoform C is strongly activated by Ca2+/calmodulin in a substrate concentration-dependent manner | Rattus norvegicus | |
| Ca2+ | activation by Ca2+/calmodulin | Rattus norvegicus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 74500 | - |
x * 74500, calculated from the amino acid sequence | Rattus norvegicus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 1D-myo-inositol 1,4,5-trisphosphate | Rattus norvegicus | inositol 1,4,5-trisphosphate is a second messenger | ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate | - |
? | |
| ATP + 1D-myo-inositol 1,4,5-trisphosphate | Rattus norvegicus | IP3K isoform C: nucleocytoplasmic shuttling, particular role in nuclear inositol trisphosphate phosphorylation and cellular growth, enzyme seems to function during the intervalls between signaling events, keeps the resting Ins(1,4,5)P3 very low and provides a basal Ins(1,3,4,5)P4 production in the absence of stimulatory signals from the exterior | ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate | - |
? | |
| ATP + 1D-myo-inositol 1,4,5-trisphosphate | Rattus norvegicus | inositol phosphate metabolism | ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
isoform C | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | isoform C | Rattus norvegicus | - |
| heart | isoform C | Rattus norvegicus | - |
| kidney | isoform C, low expression | Rattus norvegicus | - |
| liver | isoform C, low expression | Rattus norvegicus | - |
| lung | isoform C, low expression | Rattus norvegicus | - |
| testis | isoform C | Rattus norvegicus | - |
| tongue | strongest expression of isoform C in tongue epithelium | Rattus norvegicus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 1D-myo-inositol 1,4,5-trisphosphate | domain structure of IP3K-C | Rattus norvegicus | ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate | - |
? | |
| ATP + 1D-myo-inositol 1,4,5-trisphosphate | specific for phosphorylation of the 3-position | Rattus norvegicus | ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate | - |
? | |
| ATP + 1D-myo-inositol 1,4,5-trisphosphate | inositol 1,4,5-trisphosphate is a second messenger | Rattus norvegicus | ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate | - |
? | |
| ATP + 1D-myo-inositol 1,4,5-trisphosphate | IP3K isoform C: nucleocytoplasmic shuttling, particular role in nuclear inositol trisphosphate phosphorylation and cellular growth, enzyme seems to function during the intervalls between signaling events, keeps the resting Ins(1,4,5)P3 very low and provides a basal Ins(1,3,4,5)P4 production in the absence of stimulatory signals from the exterior | Rattus norvegicus | ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate | - |
? | |
| ATP + 1D-myo-inositol 1,4,5-trisphosphate | inositol phosphate metabolism | Rattus norvegicus | ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate | - |
? | |
| ATP + 1D-myo-inositol 2,4,5-trisphosphate | recombinant, catalytically active fragment of isoform C, in the presence of Ins(1,3,4,5)P4, authentic side activity of isoform C | Rattus norvegicus | ADP + 1D-myo-inositol 2,4,5,6-tetrakisphosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 74500, calculated from the amino acid sequence | Rattus norvegicus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Rattus norvegicus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Rattus norvegicus | |
| Calmodulin | activation by Ca2+/calmodulin | Rattus norvegicus | |
| Calmodulin | isoform C is strongly activated by Ca2+/calmodulin in a substrate concentration-dependent manner | Rattus norvegicus | |
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