Activating Compound | Comment | Organism | Structure |
---|---|---|---|
CaM kinase II | phosphorylates and activates | Rattus norvegicus | |
CaM kinase II | phosphorylation of Thr-311 results in 8-10fold enzyme activation in the presence of 0.01 mM free Ca2+ and 0.002 mM calmodulin and in a 25fold increase in sensitivity to the Ca2+/calmodulin complex | Homo sapiens | |
Carbachol | - |
Homo sapiens | |
Carbachol | 3-5fold activation, maximal at 0.01-0.05 mM, isoenzyme A, increases the phosphorylation of enzyme | Rattus norvegicus | |
UTP | - |
Rattus norvegicus | |
UTP | activation, recombinant isoenzyme A overexpressed in CHO cells | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
isoenzyme A is cloned and overexpressed in CHO cells | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
KN-62 | calmodulin-dependent protein kinase II inhibitor; prevents the carbachol- or UTP-mediated activation | Homo sapiens | |
KN-62 | calmodulin-dependent protein kinase II inhibitor; prevents the carbachol- or UTP-mediated activation | Rattus norvegicus | |
KN-93 | calmodulin-dependent protein kinase II inhibitor; prevents the carbachol- or UTP-mediated activation | Homo sapiens | |
KN-93 | calmodulin-dependent protein kinase II inhibitor; prevents the carbachol- or UTP-mediated activation | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.004 | - |
1D-myo-inositol 1,4,5-trisphosphate | pH 7.4 | Homo sapiens | |
0.004 | - |
1D-myo-inositol 1,4,5-trisphosphate | pH 7.5 | Rattus norvegicus | |
0.004 | - |
1D-myo-inositol 1,4,5-trisphosphate | 37°C, isoform A | Homo sapiens | |
0.004 | - |
1D-myo-inositol 1,4,5-trisphosphate | 37°C, isoform A | Rattus norvegicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | phosphorylation of Thr-311 of isoenzyme A by CaM kinase II results in a 25fold increase in sensitivity to the Ca2+/calmodulin complex | Homo sapiens | |
Ca2+ | activation by Ca2+/calmodulin | Homo sapiens | |
Ca2+ | activation by Ca2+/calmodulin | Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
53000 | - |
x * 53000, isoenzyme A, SDS-PAGE | Homo sapiens |
53000 | - |
x * 53000, isoenzyme A, SDS-PAGE | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 1D-myo-inositol 1,4,5-trisphosphate | Homo sapiens | regulation mechanism of isoenzyme A in brain involving CaM kinase II-mediated phosphorylation, role of Ins(1,4,5)P3 3-kinase in brain | ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate | - |
? | |
ATP + 1D-myo-inositol 1,4,5-trisphosphate | Rattus norvegicus | regulation mechanism of isoenzyme A in brain involving CaM kinase II-mediated phosphorylation, role of Ins(1,4,5)P3 3-kinase in brain | ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate | - |
? | |
ATP + 1D-myo-inositol 1,4,5-trisphosphate | Rattus norvegicus | inositol phosphate metabolism | ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
recombinant isoenzyme A overexpressed in CHO cells | - |
Rattus norvegicus | - |
adult | - |
Rattus norvegicus | - |
male sprague-dawley rats | - |
Rattus norvegicus | - |
isoenzyme A | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | - |
Rattus norvegicus |
phosphoprotein | phosphorylation of Thr-311 by CaM kinase II results in 8-10fold enzyme activation in the presence of 0.01 mM free Ca2+ and 0.002 mM calmodulin and in a 25fold increase in sensitivity to the Ca2+/calmodulin complex | Homo sapiens |
Purification (Comment) | Organism |
---|---|
recombinant isoenzyme A overexpressed in CHO cells | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | cortex | Rattus norvegicus | - |
brain | isoenzyme A is the major isoform present in neuronal cells | Homo sapiens | - |
brain | isoenzyme A is the major isoform present in neuronal cells | Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0048 | - |
37°C, isoenzyme A, rat cortical slices | Homo sapiens |
1.7 | - |
pH 7.4, 37°C, purified recombinant isoenzyme A overexpressed in CHO cells | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 1D-myo-inositol 1,4,5-trisphosphate | regulation mechanism of isoenzyme A involving CaM kinase II-mediated phosphorylation | Homo sapiens | ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate | - |
? | |
ATP + 1D-myo-inositol 1,4,5-trisphosphate | regulation mechanism of isoenzyme A involving CaM kinase II-mediated phosphorylation | Rattus norvegicus | ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate | - |
? | |
ATP + 1D-myo-inositol 1,4,5-trisphosphate | regulation mechanism of isoenzyme A in brain involving CaM kinase II-mediated phosphorylation, role of Ins(1,4,5)P3 3-kinase in brain | Homo sapiens | ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate | - |
? | |
ATP + 1D-myo-inositol 1,4,5-trisphosphate | regulation mechanism of isoenzyme A in brain involving CaM kinase II-mediated phosphorylation, role of Ins(1,4,5)P3 3-kinase in brain | Rattus norvegicus | ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate | - |
? | |
ATP + 1D-myo-inositol 1,4,5-trisphosphate | inositol phosphate metabolism | Rattus norvegicus | ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 53000, isoenzyme A, SDS-PAGE | Homo sapiens |
? | x * 53000, isoenzyme A, SDS-PAGE | Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
37 | - |
assay at | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Homo sapiens |
7.5 | - |
assay at | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Homo sapiens | |
ATP | - |
Rattus norvegicus | |
Calmodulin | activation by Ca2+/calmodulin | Homo sapiens | |
Calmodulin | activation by Ca2+/calmodulin | Rattus norvegicus | |
Calmodulin | phosphorylation of Thr-311 of isoenzyme A by CaM kinase II results in a 25fold increase in sensitivity to the Ca2+/calmodulin complex, maximally 2-2.5fold stimulation by the Ca2+/calmodulin complex | Homo sapiens |