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Literature summary for 2.7.1.127 extracted from

  • Dewaste, V.; Moreau, C.; De Smedt, F.; Bex, F.; De Smedt, H.; Wuytack, F.; Missiaen, L.; Erneux, C.
    The three isoenzymes of human inositol-1,4,5-trisphosphate 3-kinase show specific intracellular localization but comparable Ca2+ responses on transfection in COS-7 cells (2003), Biochem. J., 374, 41-49.
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
cloning and expression of the isoenzymes A, B and C in COS-7 cells, the 3 isoenzymes A, B and C can be distinguished by their localization on transfection in COS-7 cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information InsP3 3-kinase dead mutant cells with much lower or undetectable enzyme activities, the N-terminal deletion mutant of InsP3 3-kinase A starting at Leu-133 and the N-terminal deletion mutant of InsP3 3-kinase B starting at Asp-483 are cytoplasmic Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm isoform C is totally cytoplasmic, the N-terminal deletion mutant of InsP3 3-kinase A starting at Leu-133 and the N-terminal deletion mutant of InsP3 3-kinase B starting at Asp-483 are cytoplasmic Homo sapiens 5737
-
cytoskeleton isoform A associates with, isoform B Homo sapiens 5856
-
endoplasmic reticulum isoform B Homo sapiens 5783
-
plasma membrane isoform B Homo sapiens 5886
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ the 3 isoenzymes A, B and C can be distinguished by their sensitivity to Ca2+/calmodulin Homo sapiens
Ca2+ activation by Ca2+/calmodulin Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + 1D-myo-inositol 1,4,5-trisphosphate Homo sapiens role of the 3 isoforms as direct InsP3 metabolizing enzymes and direct regulators of Ca2+ responses to extracellular signals ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
 3 isoenzymes: A, B and C
-

Source Tissue

Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 1D-myo-inositol 1,4,5-trisphosphate
-
 Homo sapiens ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate
-
 ?
ATP + 1D-myo-inositol 1,4,5-trisphosphate role of the 3 isoforms as direct InsP3 metabolizing enzymes and direct regulators of Ca2+ responses to extracellular signals Homo sapiens ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate
-
 ?
additional information the 3 isoenzymes A, B and C can be distinguished by their N-terminal sequence, sensitivity to Ca2+/calmodulin and localization on transfection in COS-7 cells Homo sapiens ?
-
 ?

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Homo sapiens
Calmodulin activation by Ca2+/calmodulin Homo sapiens
Calmodulin the 3 isoenzymes A, B and C can be distinguished by their sensitivity to Ca2+/calmodulin Homo sapiens