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Literature summary for 2.7.1.127 extracted from

  • Johanson, R.A.; Hansen, C.A.; Williamson, J.R.
    Purification of D-myo-inositol 1,4,5-trisphosphate 3-kinase from rat brain (1988), J. Biol. Chem., 263, 7465-7471.
    View publication on PubMed
Search for more literature for 2.7.1.127

General Stability

General Stability Organism
bovine serum albumin stabilizes dilute enzyme solutions Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ at 0.1 mM, slight Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00021
-
 1D-myo-inositol 1,4,5-trisphosphate pH 7.1, 30°C, addition of Ca2+/calmodulin increases the Km 2fold Rattus norvegicus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ at physiological concentrations Rattus norvegicus
Ca2+ activation by Ca2+/calmodulin Rattus norvegicus
Ca2+ not activated by free Ca2+ Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
53000
-
 1 * 53000, SDS-PAGE Rattus norvegicus
53000
-
 2 * 53000, native enzyme consists of a dimer of 2 catalytic subunits with 1 calmodulin molecule associated with each catalytic subunit, SDS-PAGE Rattus norvegicus
70000
-
 gel filtration Rattus norvegicus
150000 160000 in the presence of Ca2+/calmodulin, gel filtration, the interaction of calmodulin with the monomeric catalytic subunit promotes dimerization of the enzyme Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + 1D-myo-inositol 1,4,5-trisphosphate Rattus norvegicus important regulatory role in inositol phosphate signaling by promoting the formation of additional inositol polyphosphate isomers ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
 adult
-
Rattus norvegicus
-
 Sprague-Dawley or Lewis rats
-

Purification (Commentary)

Purification (Comment) Organism
2700fold Rattus norvegicus
partial Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3
-
 30°C Rattus norvegicus
3.5
-
 30°C, fully activated enzyme in presence of Ca2+/calmodulin Rattus norvegicus

Storage Stability

Storage Stability Organism
4°C, 20 mM Tris-HCl buffer, pH 7.2, 1 mM Mg(CH3COO)2, 1 mM DTT, 0.1 mM EGTA, 0.5 mg/ml bovine serum albumin, 3 months, less than 20% loss of activity Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 1D-myo-inositol 1,4,5-trisphosphate
-
 Rattus norvegicus ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate
-
 ?
ATP + 1D-myo-inositol 1,4,5-trisphosphate important regulatory role in inositol phosphate signaling by promoting the formation of additional inositol polyphosphate isomers Rattus norvegicus ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 53000, native enzyme consists of a dimer of 2 catalytic subunits with 1 calmodulin molecule associated with each catalytic subunit, SDS-PAGE Rattus norvegicus
monomer 1 * 53000, SDS-PAGE Rattus norvegicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
 Rattus norvegicus

pH Range

pH Minimum pH Maximum Comment Organism
7 9 about half-maximal activity at pH 7 and about 60% of maximal activity at pH 9 Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
ATP ATP-dependent Rattus norvegicus
Calmodulin activation by Ca2+/calmodulin Rattus norvegicus