BRENDA - Enzyme Database show
show all sequences of 2.7.1.113

Deoxynucleoside kinases encoded by the yaaG and yaaF genes of Bacillus subtilis. Substrate specificity and kinetic analysis of deoxyguanosine kinase with UTP as the preferred phosphate donor

Andersen, R.B.; Neuhard, J.; J. Biol. Chem. 276, 5518-5524 (2001)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression in Escherichia coli; gene yaaG of the yaaG/yaaF operon, DNA and amino acid sequence determination and analysis, overexpression in Escherichia coli
Bacillus subtilis
General Stability
General Stability
Organism
bovine serum albumin stabilizes the enzyme in solution
Bacillus subtilis
highly unstable at 0°C in diluted form, no loss of activity in the presence of bovine serum albumin
Bacillus subtilis
Inhibitors
Inhibitors
Commentary
Organism
Structure
dGMP
noncompetitive versus UTP and 2'-deoxyguanosine
Bacillus subtilis
additional information
no substrate inhibition by CTP, ATP, and GTP
Bacillus subtilis
UDP
competitive versus UTP, noncompetitive versus 2'-deoxyguanosine
Bacillus subtilis
UTP
substrate inhibition, overview
Bacillus subtilis
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0006
-
2'-deoxyguanosine
pH 8.5, 37°C, cosubstrate UTP; pH 8.5, 37°C, recombinant enzyme, with UTP as donor substrate
Bacillus subtilis
0.0017
-
2'-deoxyguanosine
pH 8.5, 37°C, recombinant enzyme, with CTP as donor substrate
Bacillus subtilis
0.006
-
UTP
pH 8.5, 37°C; pH 8.5, 37°C, recombinant enzyme, with 2'-deoxyguanosine as acceptor substrate
Bacillus subtilis
0.0065
-
2'-deoxyguanosine
pH 8.5, 37°C, cosubstrate ATP; pH 8.5, 37°C, recombinant enzyme, with ATP as donor substrate
Bacillus subtilis
0.01
-
2'-deoxyguanosine
pH 8.5, 37°C, cosubstrate GTP
Bacillus subtilis
0.0104
-
2'-deoxyguanosine
pH 8.5, 37°C, recombinant enzyme, with GTP as donor substrate
Bacillus subtilis
0.017
-
2'-deoxyguanosine
pH 8.5, 37°C, cosubstrate CTP
Bacillus subtilis
0.035
-
CTP
pH 8.5, 37°C; pH 8.5, 37°C, recombinant enzyme, with 2'-deoxyguanosine as acceptor substrate
Bacillus subtilis
0.036
-
ATP
pH 8.5, 37°C; pH 8.5, 37°C, recombinant enzyme, with 2'-deoxyguanosine as acceptor substrate
Bacillus subtilis
0.046
-
GTP
pH 8.5, 37°C; pH 8.5, 37°C, recombinant enzyme, with 2'-deoxyguanosine as acceptor substrate
Bacillus subtilis
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
MgNTP2- is most probably the true phosphate donor; required for activity; required for activity, no inhibition by excess Mg2+, at 5 mM
Bacillus subtilis
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
24147
-
2 * 24147, electrospray mass spectrometry; 2 * 24147, recombinant enzyme, electrospray mass spectrometry
Bacillus subtilis
49000
-
recombinant deoxyguanosine kinase, gel filtration; recombinant enzyme, gel filtration
Bacillus subtilis
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Bacillus subtilis
-
; gene yaaG
-
Purification (Commentary)
Commentary
Organism
recombinant deoxyguanosine kinase, BlueA dye affinity, phenyl-Sepharose; recombinant enzyme from Escherichia coli by affinity and phenyl resin chromatography to homogeneity
Bacillus subtilis
Reaction
Reaction
Commentary
Organism
ATP + deoxyguanosine = ADP + dGMP
sequential Bi Bi mechanism
Bacillus subtilis
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.54
-
enzyme in crude recombinant Escherichia coli cell extract, substrate 2'-deoxyguanosine
Bacillus subtilis
2.3
-
dAdo/dCyd kinase in crude recombinant Escherichia coli cell extract, substrate 2'-deoxyadenosine or 2'-deoxycytidine
Bacillus subtilis
8.1
-
purified recombinant enzyme, substrate 2'-deoxyguanosine; recombinant deoxyguanosine kinase
Bacillus subtilis
Storage Stability
Storage Stability
Organism
-20°C, more than 1 mg/ml protein, 50% glycerol, 1 year, no loss of activity
Bacillus subtilis
-20°C, purified recombinant enzyme at 1 mg/ml, Tris-HCl, pH 7.8, 50% v/v glycerol, at least 1 year without loss of activity
Bacillus subtilis
0°C, the diluted enzyme is highly unstable, but in presence of 1 mg/ml bovine serum albumin, the diluted enzyme is stable for months
Bacillus subtilis
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + 2'-deoxyguanosine
-
640561
Bacillus subtilis
ADP + 2'-deoxyguanosine 5'-phosphate
-
-
-
?
ATP + deoxyadenosine
3% of activity with deoxyguanosine
640561
Bacillus subtilis
ADP + dAMP
-
-
-
?
ATP + deoxycytidine
recombinant deoxyguanosine kinase, 3% of activity with deoxyguanosine
640561
Bacillus subtilis
ADP + dCMP
-
-
-
?
ATP + deoxyinosine
63% of activity with deoxyguanosine
640561
Bacillus subtilis
ADP + dIMP
-
640561
Bacillus subtilis
?
ATP + guanosine
recombinant deoxyguanosine kinase, 7% of activity with deoxyguanosine
640561
Bacillus subtilis
ADP + GMP
-
640561
Bacillus subtilis
?
CTP + 2'-deoxyguanosine
-
640561
Bacillus subtilis
CDP + 2'-deoxyguanosine 5'-phosphate
-
-
-
?
GTP + 2'-deoxyadenosine
very low activity
640561
Bacillus subtilis
GDP + 2'-deoxyadenosine 5'-phosphate
-
-
-
?
GTP + 2'-deoxycytidine
very low activity
640561
Bacillus subtilis
GDP + 2'-deoxycytidine 5'-phosphate
-
-
-
?
GTP + 2'-deoxyguanosine
-
640561
Bacillus subtilis
GDP + 2'-deoxyguanosine 5'-phosphate
-
-
-
?
GTP + 2'-deoxyinosine
-
640561
Bacillus subtilis
GDP + 2'-deoxyinosine 5'-phosphate
-
-
-
?
GTP + guanosine
very low activity
640561
Bacillus subtilis
GDP + guanosine 5'-phosphate
-
-
-
?
additional information
substrate specificity, the enzyme utilizes all naturally occurring ribo-and deoxyribonucleosides triphosphates, with the exception of dGTP, as phosphate donors, and shows poor activity with thymidine, inosine, adenosine, 2,3-dideoxyguanosine, 2,3-dideoxyinosine, 2,3-dideoxyadenosine, and hypoxanthine arabinoside as acceptor substrates
640561
Bacillus subtilis
?
-
-
-
-
UTP + 2'-deoxyguanosine
preferred substrates
640561
Bacillus subtilis
UDP + 2'-deoxyguanosine 5'-phosphate
-
-
-
?
Subunits
Subunits
Commentary
Organism
dimer
2 * 24147, electrospray mass spectrometry; 2 * 24147, recombinant enzyme, electrospray mass spectrometry
Bacillus subtilis
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Bacillus subtilis
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.41
-
2'deoxyguanosine
pH 8.5, 37°C, recombinant enzyme, with UTP as donor substrate
Bacillus subtilis
1.41
-
deoxyguanosine
pH 8.5, 37°C, cosubstrate UTP
Bacillus subtilis
1.41
-
UTP
pH 8.5, 37°C, recombinant enzyme, with 2'-deoxyguanosine as acceptor substrate
Bacillus subtilis
1.53
-
2'deoxyguanosine
pH 8.5, 37°C, recombinant enzyme, with ATP as donor substrate
Bacillus subtilis
1.53
-
ATP
pH 8.5, 37°C, recombinant enzyme, with 2'-deoxyguanosine as acceptor substrate
Bacillus subtilis
1.53
-
deoxyguanosine
pH 8.5, 37°C, cosubstrate ATP
Bacillus subtilis
1.69
-
2'deoxyguanosine
pH 8.5, 37°C, recombinant enzyme, with GTP as donor substrate
Bacillus subtilis
1.69
-
deoxyguanosine
pH 8.5, 37°C, cosubstrate GTP
Bacillus subtilis
1.69
-
GTP
pH 8.5, 37°C, recombinant enzyme, with 2'-deoxyguanosine as acceptor substrate
Bacillus subtilis
2.25
-
2'deoxyguanosine
pH 8.5, 37°C, recombinant enzyme, with CTP as donor substrate
Bacillus subtilis
2.25
-
CTP
pH 8.5, 37°C, recombinant enzyme, with 2'-deoxyguanosine as acceptor substrate
Bacillus subtilis
2.25
-
deoxyguanosine
pH 8.5, 37°C, cosubstrate CTP
Bacillus subtilis
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9
-
; broad optimum
Bacillus subtilis
pH Range
pH Minimum
pH Maximum
Commentary
Organism
5.6
9
more than 80% of maximal activity at pH 7.5 and pH 11.5, respectively, 60% of maximal activity at pH 6.0, complete loss of activity below pH 5.6
Bacillus subtilis
6
11.5
over 80% of maximal activity at pH 7.5 and pH 11.5, about 60% of maximal activity at pH 6.0, enzyme is completely inactive below pH 5.6
Bacillus subtilis
Cofactor
Cofactor
Commentary
Organism
Structure
ATP
-
Bacillus subtilis
CTP
-
Bacillus subtilis
GTP
-
Bacillus subtilis
additional information
the enzyme utilizes all naturally occurring ribo-and deoxyribonucleosides triphosphates, with the exception of dGTP, as phosphate donors
Bacillus subtilis
UTP
-
Bacillus subtilis
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
additional information
-
additional information
product inhibition of the enzyme, overview
Bacillus subtilis
0.063
-
UDP
pH 8.5, 37°C, cosubstrate UTP
Bacillus subtilis
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli; gene yaaG of the yaaG/yaaF operon, DNA and amino acid sequence determination and analysis, overexpression in Escherichia coli
Bacillus subtilis
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
ATP
-
Bacillus subtilis
CTP
-
Bacillus subtilis
GTP
-
Bacillus subtilis
additional information
the enzyme utilizes all naturally occurring ribo-and deoxyribonucleosides triphosphates, with the exception of dGTP, as phosphate donors
Bacillus subtilis
UTP
-
Bacillus subtilis
General Stability (protein specific)
General Stability
Organism
bovine serum albumin stabilizes the enzyme in solution
Bacillus subtilis
highly unstable at 0°C in diluted form, no loss of activity in the presence of bovine serum albumin
Bacillus subtilis
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
dGMP
noncompetitive versus UTP and 2'-deoxyguanosine
Bacillus subtilis
additional information
no substrate inhibition by CTP, ATP, and GTP
Bacillus subtilis
UDP
competitive versus UTP, noncompetitive versus 2'-deoxyguanosine
Bacillus subtilis
UTP
substrate inhibition, overview
Bacillus subtilis
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
additional information
-
additional information
product inhibition of the enzyme, overview
Bacillus subtilis
0.063
-
UDP
pH 8.5, 37°C, cosubstrate UTP
Bacillus subtilis
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0006
-
2'-deoxyguanosine
pH 8.5, 37°C, cosubstrate UTP; pH 8.5, 37°C, recombinant enzyme, with UTP as donor substrate
Bacillus subtilis
0.0017
-
2'-deoxyguanosine
pH 8.5, 37°C, recombinant enzyme, with CTP as donor substrate
Bacillus subtilis
0.006
-
UTP
pH 8.5, 37°C; pH 8.5, 37°C, recombinant enzyme, with 2'-deoxyguanosine as acceptor substrate
Bacillus subtilis
0.0065
-
2'-deoxyguanosine
pH 8.5, 37°C, cosubstrate ATP; pH 8.5, 37°C, recombinant enzyme, with ATP as donor substrate
Bacillus subtilis
0.01
-
2'-deoxyguanosine
pH 8.5, 37°C, cosubstrate GTP
Bacillus subtilis
0.0104
-
2'-deoxyguanosine
pH 8.5, 37°C, recombinant enzyme, with GTP as donor substrate
Bacillus subtilis
0.017
-
2'-deoxyguanosine
pH 8.5, 37°C, cosubstrate CTP
Bacillus subtilis
0.035
-
CTP
pH 8.5, 37°C; pH 8.5, 37°C, recombinant enzyme, with 2'-deoxyguanosine as acceptor substrate
Bacillus subtilis
0.036
-
ATP
pH 8.5, 37°C; pH 8.5, 37°C, recombinant enzyme, with 2'-deoxyguanosine as acceptor substrate
Bacillus subtilis
0.046
-
GTP
pH 8.5, 37°C; pH 8.5, 37°C, recombinant enzyme, with 2'-deoxyguanosine as acceptor substrate
Bacillus subtilis
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
MgNTP2- is most probably the true phosphate donor; required for activity; required for activity, no inhibition by excess Mg2+, at 5 mM
Bacillus subtilis
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
24147
-
2 * 24147, electrospray mass spectrometry; 2 * 24147, recombinant enzyme, electrospray mass spectrometry
Bacillus subtilis
49000
-
recombinant deoxyguanosine kinase, gel filtration; recombinant enzyme, gel filtration
Bacillus subtilis
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant deoxyguanosine kinase, BlueA dye affinity, phenyl-Sepharose; recombinant enzyme from Escherichia coli by affinity and phenyl resin chromatography to homogeneity
Bacillus subtilis
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.54
-
enzyme in crude recombinant Escherichia coli cell extract, substrate 2'-deoxyguanosine
Bacillus subtilis
2.3
-
dAdo/dCyd kinase in crude recombinant Escherichia coli cell extract, substrate 2'-deoxyadenosine or 2'-deoxycytidine
Bacillus subtilis
8.1
-
purified recombinant enzyme, substrate 2'-deoxyguanosine; recombinant deoxyguanosine kinase
Bacillus subtilis
Storage Stability (protein specific)
Storage Stability
Organism
-20°C, more than 1 mg/ml protein, 50% glycerol, 1 year, no loss of activity
Bacillus subtilis
-20°C, purified recombinant enzyme at 1 mg/ml, Tris-HCl, pH 7.8, 50% v/v glycerol, at least 1 year without loss of activity
Bacillus subtilis
0°C, the diluted enzyme is highly unstable, but in presence of 1 mg/ml bovine serum albumin, the diluted enzyme is stable for months
Bacillus subtilis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + 2'-deoxyguanosine
-
640561
Bacillus subtilis
ADP + 2'-deoxyguanosine 5'-phosphate
-
-
-
?
ATP + deoxyadenosine
3% of activity with deoxyguanosine
640561
Bacillus subtilis
ADP + dAMP
-
-
-
?
ATP + deoxycytidine
recombinant deoxyguanosine kinase, 3% of activity with deoxyguanosine
640561
Bacillus subtilis
ADP + dCMP
-
-
-
?
ATP + deoxyinosine
63% of activity with deoxyguanosine
640561
Bacillus subtilis
ADP + dIMP
-
640561
Bacillus subtilis
?
ATP + guanosine
recombinant deoxyguanosine kinase, 7% of activity with deoxyguanosine
640561
Bacillus subtilis
ADP + GMP
-
640561
Bacillus subtilis
?
CTP + 2'-deoxyguanosine
-
640561
Bacillus subtilis
CDP + 2'-deoxyguanosine 5'-phosphate
-
-
-
?
GTP + 2'-deoxyadenosine
very low activity
640561
Bacillus subtilis
GDP + 2'-deoxyadenosine 5'-phosphate
-
-
-
?
GTP + 2'-deoxycytidine
very low activity
640561
Bacillus subtilis
GDP + 2'-deoxycytidine 5'-phosphate
-
-
-
?
GTP + 2'-deoxyguanosine
-
640561
Bacillus subtilis
GDP + 2'-deoxyguanosine 5'-phosphate
-
-
-
?
GTP + 2'-deoxyinosine
-
640561
Bacillus subtilis
GDP + 2'-deoxyinosine 5'-phosphate
-
-
-
?
GTP + guanosine
very low activity
640561
Bacillus subtilis
GDP + guanosine 5'-phosphate
-
-
-
?
additional information
substrate specificity, the enzyme utilizes all naturally occurring ribo-and deoxyribonucleosides triphosphates, with the exception of dGTP, as phosphate donors, and shows poor activity with thymidine, inosine, adenosine, 2,3-dideoxyguanosine, 2,3-dideoxyinosine, 2,3-dideoxyadenosine, and hypoxanthine arabinoside as acceptor substrates
640561
Bacillus subtilis
?
-
-
-
-
UTP + 2'-deoxyguanosine
preferred substrates
640561
Bacillus subtilis
UDP + 2'-deoxyguanosine 5'-phosphate
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 24147, electrospray mass spectrometry; 2 * 24147, recombinant enzyme, electrospray mass spectrometry
Bacillus subtilis
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Bacillus subtilis
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.41
-
2'deoxyguanosine
pH 8.5, 37°C, recombinant enzyme, with UTP as donor substrate
Bacillus subtilis
1.41
-
deoxyguanosine
pH 8.5, 37°C, cosubstrate UTP
Bacillus subtilis
1.41
-
UTP
pH 8.5, 37°C, recombinant enzyme, with 2'-deoxyguanosine as acceptor substrate
Bacillus subtilis
1.53
-
2'deoxyguanosine
pH 8.5, 37°C, recombinant enzyme, with ATP as donor substrate
Bacillus subtilis
1.53
-
ATP
pH 8.5, 37°C, recombinant enzyme, with 2'-deoxyguanosine as acceptor substrate
Bacillus subtilis
1.53
-
deoxyguanosine
pH 8.5, 37°C, cosubstrate ATP
Bacillus subtilis
1.69
-
2'deoxyguanosine
pH 8.5, 37°C, recombinant enzyme, with GTP as donor substrate
Bacillus subtilis
1.69
-
deoxyguanosine
pH 8.5, 37°C, cosubstrate GTP
Bacillus subtilis
1.69
-
GTP
pH 8.5, 37°C, recombinant enzyme, with 2'-deoxyguanosine as acceptor substrate
Bacillus subtilis
2.25
-
2'deoxyguanosine
pH 8.5, 37°C, recombinant enzyme, with CTP as donor substrate
Bacillus subtilis
2.25
-
CTP
pH 8.5, 37°C, recombinant enzyme, with 2'-deoxyguanosine as acceptor substrate
Bacillus subtilis
2.25
-
deoxyguanosine
pH 8.5, 37°C, cosubstrate CTP
Bacillus subtilis
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9
-
; broad optimum
Bacillus subtilis
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
5.6
9
more than 80% of maximal activity at pH 7.5 and pH 11.5, respectively, 60% of maximal activity at pH 6.0, complete loss of activity below pH 5.6
Bacillus subtilis
6
11.5
over 80% of maximal activity at pH 7.5 and pH 11.5, about 60% of maximal activity at pH 6.0, enzyme is completely inactive below pH 5.6
Bacillus subtilis
Other publictions for EC 2.7.1.113
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
738348
Vilarinho
Recurrent recessive mutation i ...
Homo sapiens
Hepatology
63
1977-1986
2016
-
-
-
-
1
-
-
-
1
-
-
1
-
3
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
737913
Li
-
One-pot process of 2-deoxyguan ...
Bacillus subtilis, Bacillus subtilis 168
Biotechnol. Bioprocess Eng.
20
37-43
2015
-
-
1
-
-
-
-
-
-
-
-
4
-
2
-
-
-
-
-
-
1
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
1
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
737461
Sun
Zidovudine induces downregulat ...
Homo sapiens
Antimicrob. Agents Chemother.
58
6758-6766
2014
-
-
-
-
-
-
-
-
1
-
-
1
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
737599
Sun
Down-regulation of mitochondri ...
Homo sapiens
Biochem. Biophys. Res. Commun.
450
1021-1026
2014
-
-
-
-
-
-
-
-
1
-
-
1
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
723198
Buchaklian
Recessive deoxyguanosine kinas ...
Homo sapiens
Mol. Genet. Metab.
107
92-94
2012
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
722470
Pronicka
Post mortem identification of ...
Homo sapiens
J. Appl. Genet.
52
61-66
2011
-
-
-
-
4
-
-
-
-
-
-
-
-
2
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
703966
Dimmock
Clinical and molecular feature ...
Homo sapiens
Hum. Mutat.
29
330-331
2008
-
-
-
-
8
-
-
-
1
-
-
-
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
8
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
705474
Dimmock
Abnormal neurological features ...
Homo sapiens
Liver Transpl.
14
1480-1485
2008
-
-
-
-
6
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
705750
Saada
Mitochondrial deoxyribonucleot ...
Homo sapiens
Mol. Genet. Metab.
95
169-173
2008
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
705997
Fyrberg
RNAi depletion of deoxycytidin ...
Homo sapiens
Nucleosides Nucleotides Nucleic Acids
27
712-719
2008
-
-
-
-
-
-
6
-
-
-
-
-
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
671801
Fyrberg
Cell cycle effect on the activ ...
Homo sapiens
Biochem. Biophys. Res. Commun.
357
847-853
2007
-
-
-
-
-
-
-
-
1
-
-
1
-
2
-
-
-
-
-
5
-
-
3
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
5
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
671890
Mousson de Camaret
Kinetic properties of mutant d ...
Homo sapiens
Biochem. J.
402
377-385
2007
-
-
-
-
5
-
-
10
1
-
-
2
-
2
-
-
-
-
-
2
-
1
4
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
5
-
-
-
-
10
1
-
-
2
-
-
-
-
-
2
-
1
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
673472
Franco
Depletion of mitochondrial DNA ...
Homo sapiens
Exp. Cell Res.
313
2687-2694
2007
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
675444
Sandrini
Dictyostelium discoideum salva ...
Dictyostelium discoideum
J. Mol. Biol.
369
653-664
2007
-
-
1
-
-
-
-
2
-
-
1
-
-
6
-
-
-
-
-
1
-
-
2
-
-
-
-
2
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
1
-
-
2
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
671919
Lotfi
The pattern of deoxycytidine- ...
Homo sapiens
Biochem. Pharmacol.
71
882-890
2006
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
5
3
-
6
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
5
3
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
661040
Rylova
Activity profiles of deoxynucl ...
Homo sapiens
Biochem. Pharmacol.
69
951-960
2005
-
-
1
-
-
-
-
-
1
1
-
1
-
2
-
-
-
-
-
3
2
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
1
1
-
1
-
-
-
-
-
3
2
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
662882
Wang
Molecular insight into mitocho ...
Homo sapiens
Mol. Genet. Metab.
84
75-82
2005
-
-
1
-
1
-
-
9
2
1
-
3
-
2
-
-
-
-
-
2
1
-
5
-
-
-
-
-
1
-
-
1
-
-
-
-
-
1
1
-
1
-
-
-
-
9
2
1
-
3
-
-
-
-
-
2
1
-
5
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
661034
Feng
Anabolism of amdoxovir: phosph ...
Homo sapiens
Biochem. Pharmacol.
68
1879-1888
2004
-
-
-
-
-
-
-
2
-
1
-
3
-
6
-
-
-
-
-
-
-
-
4
-
1
-
-
2
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
2
-
1
-
3
-
-
-
-
-
-
-
-
4
-
1
-
-
2
1
-
-
-
-
-
-
-
-
-
661731
Piskur
Animal deoxyribonucleoside kin ...
Homo sapiens
FEBS Lett.
560
3-6
2004
-
-
-
-
-
-
-
-
1
1
-
4
-
1
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
1
-
4
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
662992
Al-Madhoun
Phosphorylation of isocarbosty ...
Homo sapiens
Nucleosides Nucleotides Nucleic Acids
23
1865-1874
2004
-
-
-
-
-
-
-
2
3
1
-
1
-
2
-
-
-
-
-
-
1
-
4
-
1
-
-
2
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
2
3
1
-
1
-
-
-
-
-
-
1
-
4
-
1
-
-
2
1
-
-
-
-
-
-
-
-
-
660855
Krawiec
Inorganic tripolyphosphate (PP ...
Homo sapiens
Biochem. Biophys. Res. Commun.
301
192-197
2003
-
-
-
-
-
1
1
3
4
1
-
1
-
3
-
-
-
-
-
-
1
-
3
-
1
-
-
-
1
-
-
2
-
-
-
-
-
-
2
-
-
1
-
1
-
3
4
1
-
1
-
-
-
-
-
-
1
-
3
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
661728
Wang
Mitochondrial deoxyguanosine k ...
Homo sapiens
FEBS Lett.
554
319-322
2003
-
-
1
-
3
-
-
10
1
1
-
3
-
1
-
-
-
1
-
-
1
-
3
-
-
-
-
-
1
-
-
1
-
-
-
-
-
1
1
-
3
-
-
-
-
10
1
1
-
3
-
-
-
-
-
-
1
-
3
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
662985
Krawiec
Ability of adenosine-2'(3')-de ...
Homo sapiens
Nucleosides Nucleotides Nucleic Acids
22
1525-1529
2003
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
1
-
7
-
-
-
-
-
-
-
-
7
-
-
-
-
-
-
7
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
7
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
663023
Van Rompay
Substrate specificity and phos ...
Homo sapiens
Pharmacol. Ther.
100
119-139
2003
-
1
1
-
-
-
-
-
2
1
-
4
-
1
-
-
-
-
-
5
-
-
9
-
-
-
-
-
-
-
-
3
-
-
-
-
1
1
3
-
-
-
-
-
-
-
2
1
-
4
-
-
-
-
-
5
-
-
9
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
640563
Lotfi
Low level of mitochondrial deo ...
Homo sapiens
Biochem. Biophys. Res. Commun.
293
1489-1496
2002
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
640564
Park
Mutations within the putative ...
Lactobacillus johnsonii, Lactobacillus johnsonii NCC 533
J. Biochem. Mol. Biol.
35
244-247
2002
-
-
-
-
3
-
1
4
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
1
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
640568
Rodriguez
Arabinosylguanine is phosphory ...
Homo sapiens
Cancer Res.
62
3100-3105
2002
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
661453
Eriksson
Structure and function of cell ...
Homo sapiens
Cell. Mol. Life Sci.
59
1327-1346
2002
1
-
1
1
-
1
2
-
3
1
1
4
-
1
-
-
1
1
-
5
-
-
14
2
-
-
-
-
-
-
-
3
-
-
-
1
-
1
3
1
-
1
-
2
-
-
3
1
1
4
-
-
-
1
-
5
-
-
14
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
640560
Jullig
Apoptosis induces efflux of th ...
Homo sapiens
J. Biol. Chem.
276
24000-24004
2001
-
-
-
-
-
-
-
-
1
-
-
-
-
2
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
640561
Andersen
Deoxynucleoside kinases encode ...
Bacillus subtilis
J. Biol. Chem.
276
5518-5524
2001
-
-
1
-
-
2
4
10
-
1
2
-
-
3
-
-
1
1
-
-
3
3
13
1
1
-
-
12
1
2
-
5
2
-
-
-
-
1
5
-
-
2
-
4
2
10
-
1
2
-
-
-
-
1
-
-
3
3
13
1
1
-
-
12
1
2
-
-
-
-
-
-
-
-
640562
Herrstrom Sjoberg
Antiviral guanosine analogs as ...
Bos taurus, Homo sapiens
Antimicrob. Agents Chemother.
45
739-742
2001
-
-
1
-
-
-
5
4
-
-
-
-
-
6
-
-
-
-
-
4
-
-
5
-
-
-
-
-
-
-
-
-
5
-
-
-
-
1
-
-
-
-
-
5
5
4
-
-
-
-
-
-
-
-
-
4
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
662932
Johansson
Structural basis for substrate ...
Homo sapiens
Nat. Struct. Biol.
8
616-620
2001
-
1
-
1
-
-
-
-
-
1
-
-
-
3
-
-
-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
640559
Jullig
Mitochondrial and submitochond ...
Homo sapiens
Eur. J. Biochem.
267
5466-5472
2000
-
-
-
-
-
-
-
-
3
-
-
-
-
2
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
640557
Gaubert
Low enantioselectivities of hu ...
Homo sapiens
Biochimie
81
1041-1047
1999
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
640558
Petrakis
Cloning and characterization o ...
Mus musculus
J. Biol. Chem.
274
24726-24730
1999
-
-
1
-
-
-
-
-
3
-
-
-
-
3
-
-
-
-
-
4
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
4
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
640555
Sjoberg
Substrate specificity of human ...
Homo sapiens
Mol. Pharmacol.
53
270-273
1998
-
-
1
-
-
-
-
8
2
-
-
1
-
3
-
-
1
-
-
-
1
-
10
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
8
2
-
-
1
-
-
-
1
-
-
1
-
10
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
640556
Zhu
Enhanced cytotoxicity of nucle ...
Homo sapiens
J. Biol. Chem.
273
14707-14711
1998
-
-
1
-
-
-
-
-
2
-
-
-
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
640554
Park
Kinetic mechanism and end-prod ...
Bos taurus
J. Biochem.
117
1058-1061
1995
-
-
-
-
-
-
7
2
2
-
-
-
-
2
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
16
-
-
-
-
-
-
-
-
-
-
7
16
2
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
640565
Hong
Directed mutagenesis of deoxyg ...
Lactobacillus acidophilus
J. Biol. Chem.
270
6602-6606
1995
-
-
-
-
4
-
1
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
4
-
-
1
2
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
640567
Ma
Cloning and expression of the ...
Lactobacillus johnsonii, Lactobacillus johnsonii NCC 533
J. Biol. Chem.
270
6595-6601
1995
-
-
1
-
-
-
1
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
640566
Ikeda
Heterodimeric deoxynucleoside ...
Lactobacillus acidophilus
Biochemistry
33
5328-5334
1994
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
640551
Park
Properties of a highly purifie ...
Bos taurus
Arch. Biochem. Biophys.
266
51-60
1988
1
-
-
-
-
1
14
5
2
8
2
-
-
2
-
-
1
-
-
2
-
-
13
1
1
-
3
-
2
1
3
-
3
1
-
1
-
-
-
-
-
1
-
14
3
5
2
8
2
-
-
-
-
1
-
2
-
-
13
1
1
-
3
-
2
1
3
1
-
-
-
-
-
-
640553
Yamada
Deoxyguanosine kinase from hum ...
Homo sapiens
Biochim. Biophys. Acta
709
265-272
1982
5
-
-
-
-
2
24
4
-
10
2
-
-
2
-
-
1
-
-
2
1
1
11
1
-
-
-
-
3
-
-
-
-
-
-
5
-
-
-
-
-
2
-
24
-
4
-
10
2
-
-
-
-
1
-
2
1
1
11
1
-
-
-
-
3
-
-
-
-
-
-
-
-
-
640549
Barker
Deoxyguanosine kinase of neona ...
Mus musculus
Biochim. Biophys. Acta
658
111-123
1981
-
-
-
-
-
2
8
3
1
5
1
1
-
2
-
-
1
-
-
2
1
1
12
-
-
-
-
-
1
1
-
-
5
-
-
-
-
-
-
-
-
2
-
8
5
3
1
5
1
1
-
-
-
1
-
2
1
1
12
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
640550
Gower
Deoxyguanosine kinase. Distinc ...
Bos taurus
J. Biol. Chem.
254
2180-2183
1979
7
-
-
-
-
-
5
1
1
1
-
1
-
1
-
-
1
-
-
1
-
-
7
-
-
-
-
-
-
-
-
-
-
1
-
7
-
-
-
-
-
-
-
5
-
1
1
1
-
1
-
-
-
1
-
1
-
-
7
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
640552
Green
Partial purification and chara ...
Sus scrofa
Biochem. J.
183
547-553
1979
-
-
-
-
-
-
16
2
-
5
1
-
-
2
-
-
1
-
-
2
1
-
5
-
1
1
-
-
1
1
-
-
3
-
-
-
-
-
-
-
-
-
-
16
3
2
-
5
1
-
-
-
-
1
-
2
1
-
5
-
1
1
-
-
1
1
-
-
-
-
-
-
-
-