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Literature summary for 2.7.1.113 extracted from

  • Yamada, Y.; Goto, H.; Ogasawara, N.
    Deoxyguanosine kinase from human placenta (1982), Biochim. Biophys. Acta, 709, 265-272.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
CTP activation, with ATP as substrate at pH 7 Homo sapiens
dTTP 1 mM, 3-4fold activation of deoxyguanosine kinase at pH 7.0 Homo sapiens
dUTP activation, with ATP as substrate, at pH 7 Homo sapiens
additional information not activated by dTDP Homo sapiens
UTP slight inhibition at pH 6 Homo sapiens
UTP stimulation with ATP as substrate, at pH 7 Homo sapiens

General Stability

General Stability Organism
complete inactivation after freezing at -20°C or -70°C Homo sapiens
loss of activity in dilute solution Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
ADP 1 mM, 45% inhibition at pH 6.0 Homo sapiens
arabinoadenosine
-
Homo sapiens
arabinocytidine
-
Homo sapiens
CTP
-
Homo sapiens
CuSO4
-
Homo sapiens
dADP 1 mM, 49% inhibition at pH 6.0 Homo sapiens
dATP 1 mM, 71% inhibition at pH 6.0 Homo sapiens
dCDP
-
Homo sapiens
dCTP
-
Homo sapiens
deoxyadenosine
-
Homo sapiens
deoxyinosine
-
Homo sapiens
dGDP 0.0021 mM, 50% inhibition, reversed by 0.2 mM dTTP Homo sapiens
dGMP 1 mM, 64% inhibition at pH 6.0 Homo sapiens
dGTP 0.001 mM, 50% inhibition, 1 mM, complete inhibition, reversed by 0.2 mM dTTP Homo sapiens
dTDP 1 mM, 51% inhibition at pH 6.0 Homo sapiens
dUTP
-
Homo sapiens
EDTA
-
Homo sapiens
GDP 1 mM, 50% inhibition at pH 6.0 Homo sapiens
GTP
-
Homo sapiens
guanosine
-
Homo sapiens
IDP 1 mM, 58% inhibition at pH 6.0 Homo sapiens
additional information not inhibited by deoxycytidine, adenosine; not inhibited by dTTP; not inhibited by inosine, cytidine, uridine, deoxythymidine, deoxyuridine Homo sapiens
ribavirin
-
Homo sapiens
UTP
-
Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0025
-
deoxyguanosine pH 6.0, 37°C Homo sapiens
0.13
-
ATP pH 6.0, 37°C Homo sapiens
0.13
-
ATP ATP in form of MgATP2- Homo sapiens
0.63
-
deoxyadenosine pH 6.0, 37°C Homo sapiens
2.2
-
dTTP pH 6.0, 37°C Homo sapiens
2.2
-
dTTP dTTP in form of MgdTTP2- Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Ba2+ slight activation Homo sapiens
Ca2+ activation Homo sapiens
Ca2+ 60% of activity with Mg2+ Homo sapiens
Co2+ 59% of activity with Mg2+ Homo sapiens
Cr2+ slight activation Homo sapiens
Fe2+ 33% of activity with Mg2+ Homo sapiens
Mg2+ required for activity Homo sapiens
Mn2+ activation, as effective as Mg2+ Homo sapiens
additional information not activated by Cu2+ Homo sapiens
Ni2+ slight activation Homo sapiens
Zn2+ 45% of activity with Mg2+ Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
29000
-
2 * 29000, second mayor band at 35000 Da, SDS-PAGE Homo sapiens
58000
-
gel filtration Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate, affinity chromatography on AMP-Sepharose and Blue-Sepharose, DEAE-cellulose, Sephadex G-75 Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
placenta
-
Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0103
-
-
Homo sapiens

Storage Stability

Storage Stability Organism
4°C, 1 week, 50% loss of activity Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + deoxyadenosine 4.8%, 25.8% and 93.5% of activity with deoxyguanosine at 0.01 mM, 0.1 mM and 1 mM deoxyguanosine, respectively Homo sapiens ADP + dAMP
-
?
ATP + deoxyguanosine no activity with deoxycytidine Homo sapiens ADP + dGMP
-
?
ATP + deoxyguanosine ATP can be replaced by dUTP Homo sapiens ADP + dGMP
-
?
ATP + deoxyguanosine no activity with dATP and GTP Homo sapiens ADP + dGMP
-
?
ATP + deoxyguanosine no activity with dGTP Homo sapiens ADP + dGMP
-
?
ATP + deoxyguanosine ATP can be replaced by dTTP, CTP, dCTP and UTP Homo sapiens ADP + dGMP
-
?
CTP + deoxyguanosine 62% of activity with ATP at pH 6.0, 1.8fold higher activity than with ATP at pH 7.0 Homo sapiens CDP + dGMP
-
?
dCTP + deoxyguanosine
-
Homo sapiens dCDP + dGMP
-
?
dTTP + deoxyguanosine 83% of activity with ATP at pH 6.0, 2.8fold higher activity than with ATP at pH 7.0 Homo sapiens dTDP + dGMP
-
?
dTTP + deoxyguanosine
-
Homo sapiens ?
-
?
dUTP + deoxyguanosine 69% of activity with ATP at pH 6.0, 1.7fold higher activity than with ATP at pH 7.0 Homo sapiens dUDP + dGMP
-
?
UTP + deoxyguanosine
-
Homo sapiens UDP + dGMP
-
?

Subunits

Subunits Comment Organism
dimer 2 * 29000, second mayor band at 35000 Da, SDS-PAGE Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
cosubstrate ATP Homo sapiens
6.8
-
cosubstrate ATP, addition of dTTP Homo sapiens
7
-
cosubstrate dTTP Homo sapiens