Activating Compound | Comment | Organism | Structure |
---|---|---|---|
CTP | activation, with ATP as substrate at pH 7 | Homo sapiens | |
dTTP | 1 mM, 3-4fold activation of deoxyguanosine kinase at pH 7.0 | Homo sapiens | |
dUTP | activation, with ATP as substrate, at pH 7 | Homo sapiens | |
additional information | not activated by dTDP | Homo sapiens | |
UTP | slight inhibition at pH 6 | Homo sapiens | |
UTP | stimulation with ATP as substrate, at pH 7 | Homo sapiens |
General Stability | Organism |
---|---|
complete inactivation after freezing at -20°C or -70°C | Homo sapiens |
loss of activity in dilute solution | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ADP | 1 mM, 45% inhibition at pH 6.0 | Homo sapiens | |
arabinoadenosine | - |
Homo sapiens | |
arabinocytidine | - |
Homo sapiens | |
CTP | - |
Homo sapiens | |
CuSO4 | - |
Homo sapiens | |
dADP | 1 mM, 49% inhibition at pH 6.0 | Homo sapiens | |
dATP | 1 mM, 71% inhibition at pH 6.0 | Homo sapiens | |
dCDP | - |
Homo sapiens | |
dCTP | - |
Homo sapiens | |
deoxyadenosine | - |
Homo sapiens | |
deoxyinosine | - |
Homo sapiens | |
dGDP | 0.0021 mM, 50% inhibition, reversed by 0.2 mM dTTP | Homo sapiens | |
dGMP | 1 mM, 64% inhibition at pH 6.0 | Homo sapiens | |
dGTP | 0.001 mM, 50% inhibition, 1 mM, complete inhibition, reversed by 0.2 mM dTTP | Homo sapiens | |
dTDP | 1 mM, 51% inhibition at pH 6.0 | Homo sapiens | |
dUTP | - |
Homo sapiens | |
EDTA | - |
Homo sapiens | |
GDP | 1 mM, 50% inhibition at pH 6.0 | Homo sapiens | |
GTP | - |
Homo sapiens | |
guanosine | - |
Homo sapiens | |
IDP | 1 mM, 58% inhibition at pH 6.0 | Homo sapiens | |
additional information | not inhibited by deoxycytidine, adenosine; not inhibited by dTTP; not inhibited by inosine, cytidine, uridine, deoxythymidine, deoxyuridine | Homo sapiens | |
ribavirin | - |
Homo sapiens | |
UTP | - |
Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0025 | - |
deoxyguanosine | pH 6.0, 37°C | Homo sapiens | |
0.13 | - |
ATP | pH 6.0, 37°C | Homo sapiens | |
0.13 | - |
ATP | ATP in form of MgATP2- | Homo sapiens | |
0.63 | - |
deoxyadenosine | pH 6.0, 37°C | Homo sapiens | |
2.2 | - |
dTTP | pH 6.0, 37°C | Homo sapiens | |
2.2 | - |
dTTP | dTTP in form of MgdTTP2- | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | slight activation | Homo sapiens | |
Ca2+ | activation | Homo sapiens | |
Ca2+ | 60% of activity with Mg2+ | Homo sapiens | |
Co2+ | 59% of activity with Mg2+ | Homo sapiens | |
Cr2+ | slight activation | Homo sapiens | |
Fe2+ | 33% of activity with Mg2+ | Homo sapiens | |
Mg2+ | required for activity | Homo sapiens | |
Mn2+ | activation, as effective as Mg2+ | Homo sapiens | |
additional information | not activated by Cu2+ | Homo sapiens | |
Ni2+ | slight activation | Homo sapiens | |
Zn2+ | 45% of activity with Mg2+ | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
29000 | - |
2 * 29000, second mayor band at 35000 Da, SDS-PAGE | Homo sapiens |
58000 | - |
gel filtration | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate, affinity chromatography on AMP-Sepharose and Blue-Sepharose, DEAE-cellulose, Sephadex G-75 | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
placenta | - |
Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0103 | - |
- |
Homo sapiens |
Storage Stability | Organism |
---|---|
4°C, 1 week, 50% loss of activity | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + deoxyadenosine | 4.8%, 25.8% and 93.5% of activity with deoxyguanosine at 0.01 mM, 0.1 mM and 1 mM deoxyguanosine, respectively | Homo sapiens | ADP + dAMP | - |
? | |
ATP + deoxyguanosine | no activity with deoxycytidine | Homo sapiens | ADP + dGMP | - |
? | |
ATP + deoxyguanosine | ATP can be replaced by dUTP | Homo sapiens | ADP + dGMP | - |
? | |
ATP + deoxyguanosine | no activity with dATP and GTP | Homo sapiens | ADP + dGMP | - |
? | |
ATP + deoxyguanosine | no activity with dGTP | Homo sapiens | ADP + dGMP | - |
? | |
ATP + deoxyguanosine | ATP can be replaced by dTTP, CTP, dCTP and UTP | Homo sapiens | ADP + dGMP | - |
? | |
CTP + deoxyguanosine | 62% of activity with ATP at pH 6.0, 1.8fold higher activity than with ATP at pH 7.0 | Homo sapiens | CDP + dGMP | - |
? | |
dCTP + deoxyguanosine | - |
Homo sapiens | dCDP + dGMP | - |
? | |
dTTP + deoxyguanosine | 83% of activity with ATP at pH 6.0, 2.8fold higher activity than with ATP at pH 7.0 | Homo sapiens | dTDP + dGMP | - |
? | |
dTTP + deoxyguanosine | - |
Homo sapiens | ? | - |
? | |
dUTP + deoxyguanosine | 69% of activity with ATP at pH 6.0, 1.7fold higher activity than with ATP at pH 7.0 | Homo sapiens | dUDP + dGMP | - |
? | |
UTP + deoxyguanosine | - |
Homo sapiens | UDP + dGMP | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 29000, second mayor band at 35000 Da, SDS-PAGE | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
cosubstrate ATP | Homo sapiens |
6.8 | - |
cosubstrate ATP, addition of dTTP | Homo sapiens |
7 | - |
cosubstrate dTTP | Homo sapiens |