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Literature summary for 2.7.1.113 extracted from

  • Green, F.J.; Lewis, R.A.
    Partial purification and characterization of deoxyguanosine kinase from pig skin (1979), Biochem. J., 183, 547-553.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.1.113

Inhibitors

Inhibitors Comment Organism Structure
AMP weak inhibition Sus scrofa
ATP weak inhibition Sus scrofa
CMP weak inhibition Sus scrofa
dATP 0.1 mM, 62% inhibition Sus scrofa
dCMP weak inhibition Sus scrofa
dCTP 0.1 mM, 48% inhibition Sus scrofa
deoxyadenosine weak inhibition Sus scrofa
dGMP 0.1 mM, 44% inhibition Sus scrofa
dGTP 0.1 mM, complete inhibition Sus scrofa
dTMP weak inhibition Sus scrofa
dTTP 0.1 mM, 30% inhibition Sus scrofa
GMP weak inhibition Sus scrofa
GTP 0.1 mM, 33% inhibition Sus scrofa
additional information not inhibited by dAMP Sus scrofa
UMP 0.1 mM, 24% inhibition Sus scrofa
UTP 0.1 mM, 30% inhibition Sus scrofa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00032
-
 deoxyguanosine pH 8.0, 37°C Sus scrofa
3.3
-
 ATP pH 8.0, 37°C Sus scrofa
3.3
-
 ATP ATP in form of MgATP2- Sus scrofa

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activation Sus scrofa
Ca2+ 67% of activity with Mg2+ Sus scrofa
Fe2+ slight activation Sus scrofa
Mg2+ required for activity Sus scrofa
Mn2+ 92% of activity with Mg2+ Sus scrofa
additional information divalent cations required, not activated by Zn2+ Sus scrofa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
58500
-
 gel filtration Sus scrofa

Organism

Organism UniProt Comment Textmining
Sus scrofa
-
 piglet
-

Purification (Commentary)

Purification (Comment) Organism
pH 5.5, ammonium sulfate, DEAE-Sephadex Sus scrofa

Source Tissue

Source Tissue Comment Organism Textmining
skin
-
 Sus scrofa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0000338
-
-
 Sus scrofa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + deoxyguanosine highly specific for phospho group acceptor Sus scrofa ADP + dGMP
-
 ?
ATP + deoxyguanosine ATP twice as effective as CTP or GTP and 4 times as effective as UTP Sus scrofa ADP + dGMP
-
 ?
CTP + deoxyguanosine 50% of activity with ATP Sus scrofa CDP + dGMP
-
 ?
GTP + deoxyguanosine 50% of activity with ATP Sus scrofa GDP + dGMP
-
 ?
UTP + deoxyguanosine 25% of activity with ATP Sus scrofa UDP + dGMP
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Sus scrofa

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 53 17% loss of activity at 53°C, 50% loss of activity at 30°C and 53°C Sus scrofa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
 Sus scrofa

pH Range

pH Minimum pH Maximum Comment Organism
5 9 approx. 55% of maximal activity at pH 5.5 and pH 9, plateau with approx. 85% of maximal activity at pH 6.0-7.5 Sus scrofa

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.021
-
 dGTP pH 8.0, 37°C Sus scrofa
0.076
-
 dGMP pH 8.0, 37°C Sus scrofa
0.34
-
 deoxyadenosine pH 8.0, 37°C Sus scrofa