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Literature summary for 2.7.1.113 extracted from

  • Park, I.; Ives, D.H.
    Properties of a highly purified mitochondrial deoxyguanosine kinase (1988), Arch. Biochem. Biophys., 266, 51-60.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
Triton X-100 maximal activation at concentrations above critical micelle concentration Bos taurus

General Stability

General Stability Organism
Triton X-100, 0.02%, stabilizes markedly against thermal inactivation Bos taurus

Inhibitors

Inhibitors Comment Organism Structure
1,2-Cyclohexanedione
-
Bos taurus
1-alpha-D-arabinofuranosylguanine
-
Bos taurus
2,3-Butanedione
-
Bos taurus
8-aza-2'-deoxyguanosine
-
Bos taurus
8-azadeoxyguanosine
-
Bos taurus
arabinosylguanine
-
Bos taurus
carbodiimide
-
Bos taurus
deoxyinosine
-
Bos taurus
ethoxyformic anhydride deoxyguanosine slightly protects Bos taurus
Iodine
-
Bos taurus
additional information not inhibited by acyclovir, 6-thiodeoxyguanosine, methylacetimidate, pyridoxal phosphate; not inhibited by deoxyadenosine; not inhibited by deoxycytidine, adenosine; not inhibited by guanosine Bos taurus
N-ethylmaleimide
-
Bos taurus
p-mercuribenzoate
-
Bos taurus
Rose Bengal mediated photooxidation deoxyguanosine protects Bos taurus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0047
-
deoxyguanosine pH 7.4, 37°C, mitochondrial deoxyguanosine kinase Bos taurus
0.021
-
deoxyinosine pH 7.4, 37°C, mitochondrial deoxyguanosine kinase Bos taurus
0.08
-
dTTP pH 7.4, 37°C, mitochondrial deoxyguanosine kinase Bos taurus
0.125
-
UTP pH 7.4, 37°C, mitochondrial deoxyguanosine kinase Bos taurus
0.78
-
ATP pH 7.4, 37°C, mitochondrial deoxyguanosine kinase Bos taurus

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion membrane associated Bos taurus 5739
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activation Bos taurus
Ca2+ 52% of activity with Mg2+ Bos taurus
Cd2+ slight activation Bos taurus
Co2+ 53% of activity with Mg2+ Bos taurus
Cu2+ 25% of activity with Mg2+ Bos taurus
Fe2+ 67% of activity with Mg2+ Bos taurus
Mg2+ required for activity Bos taurus
Mn2+ activation, as effective as Mg2+ Bos taurus
Zn2+ 28% of activity with Mg2+ Bos taurus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28000
-
2 * 28000, mitochondrial deoxyguanosine kinase, SDS-PAGE Bos taurus
56000
-
mitochondrial deoxyguanosine kinase, equilibrium sedimentation centrifugation Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
mitochondrial deoxyguanosine kinase, one-step purification via deoxyguanosine-3'-(4-aminophenylphosphate)-Sepharose affinity chromatography Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Bos taurus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + deoxyguanosine no activity with deoxycytidine Bos taurus ADP + dGMP
-
?
ATP + deoxyguanosine poor donor substrates are dATP or GTP Bos taurus ADP + dGMP
-
?
ATP + deoxyguanosine no activity with dGTP Bos taurus ADP + dGMP
-
?
ATP + deoxyguanosine ATP most efficient at pH 5.5, dTTP or UTP most efficient, i.e. more efficient than ATP, at pH 7.4 Bos taurus ADP + dGMP
-
?
ATP + deoxyguanosine no activity with dITP Bos taurus ADP + dGMP
-
?
ATP + deoxyguanosine no activity with adenosine Bos taurus ADP + dGMP
-
?
ATP + deoxyguanosine ATP can be replaced by dTTP, CTP, dCTP and UTP Bos taurus ADP + dGMP
-
?
ATP + deoxyguanosine highly specific for phospho group acceptor Bos taurus ADP + dGMP
-
?
ATP + deoxyinosine
-
Bos taurus ADP + dIMP
-
?
CTP + deoxyguanosine
-
Bos taurus CDP + dGMP
-
?
dCTP + deoxyguanosine
-
Bos taurus dCDP + dGMP
-
?
dTTP + deoxyguanosine
-
Bos taurus dTDP + dGMP
-
?
UTP + deoxyguanosine
-
Bos taurus UDP + dGMP
-
?

Subunits

Subunits Comment Organism
dimer 2 * 28000, mitochondrial deoxyguanosine kinase, SDS-PAGE Bos taurus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37 40
-
Bos taurus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
Triton X-100, 0.02%, stabilizes markedly against thermal inactivation Bos taurus
37
-
at least 4 h stable in the presence of Triton X-100, t1/2: 30 min at pH 11, inactivation within 30 min at pH 5.5 Bos taurus
40
-
denaturation above 40°C, mitochondrial deoxyguanosine kinase Bos taurus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
-
Bos taurus
7.4
-
cosubstrates dTTP or UTP Bos taurus

pH Range

pH Minimum pH Maximum Comment Organism
5
-
rapid decrease of activity below Bos taurus

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.5
-
95% loss of activity after 30 min at 37°C Bos taurus
7
-
most stable Bos taurus
11
-
50% loss of activity after 30 min at 37°C Bos taurus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.075
-
deoxyinosine pH 7.4, 37°C, competitive vs. deoxyguanosine, mitochondrial deoxyguanosine kinase Bos taurus
0.117
-
1-alpha-D-arabinofuranosylguanine pH 7.4, 37°C, competitive vs. deoxyguanosine, mitochondrial deoxyguanosine kinase Bos taurus
0.43
-
8-aza-2'-deoxyguanosine pH 7.4, 37°C, competitive vs. deoxyguanosine, mitochondrial deoxyguanosine kinase Bos taurus

pI Value

Organism Comment pI Value Maximum pI Value
Bos taurus
-
-
5.9