Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Triton X-100 | maximal activation at concentrations above critical micelle concentration | Bos taurus |
General Stability | Organism |
---|---|
Triton X-100, 0.02%, stabilizes markedly against thermal inactivation | Bos taurus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,2-Cyclohexanedione | - |
Bos taurus | |
1-alpha-D-arabinofuranosylguanine | - |
Bos taurus | |
2,3-Butanedione | - |
Bos taurus | |
8-aza-2'-deoxyguanosine | - |
Bos taurus | |
8-azadeoxyguanosine | - |
Bos taurus | |
arabinosylguanine | - |
Bos taurus | |
carbodiimide | - |
Bos taurus | |
deoxyinosine | - |
Bos taurus | |
ethoxyformic anhydride | deoxyguanosine slightly protects | Bos taurus | |
Iodine | - |
Bos taurus | |
additional information | not inhibited by acyclovir, 6-thiodeoxyguanosine, methylacetimidate, pyridoxal phosphate; not inhibited by deoxyadenosine; not inhibited by deoxycytidine, adenosine; not inhibited by guanosine | Bos taurus | |
N-ethylmaleimide | - |
Bos taurus | |
p-mercuribenzoate | - |
Bos taurus | |
Rose Bengal mediated photooxidation | deoxyguanosine protects | Bos taurus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0047 | - |
deoxyguanosine | pH 7.4, 37°C, mitochondrial deoxyguanosine kinase | Bos taurus | |
0.021 | - |
deoxyinosine | pH 7.4, 37°C, mitochondrial deoxyguanosine kinase | Bos taurus | |
0.08 | - |
dTTP | pH 7.4, 37°C, mitochondrial deoxyguanosine kinase | Bos taurus | |
0.125 | - |
UTP | pH 7.4, 37°C, mitochondrial deoxyguanosine kinase | Bos taurus | |
0.78 | - |
ATP | pH 7.4, 37°C, mitochondrial deoxyguanosine kinase | Bos taurus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | membrane associated | Bos taurus | 5739 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | activation | Bos taurus | |
Ca2+ | 52% of activity with Mg2+ | Bos taurus | |
Cd2+ | slight activation | Bos taurus | |
Co2+ | 53% of activity with Mg2+ | Bos taurus | |
Cu2+ | 25% of activity with Mg2+ | Bos taurus | |
Fe2+ | 67% of activity with Mg2+ | Bos taurus | |
Mg2+ | required for activity | Bos taurus | |
Mn2+ | activation, as effective as Mg2+ | Bos taurus | |
Zn2+ | 28% of activity with Mg2+ | Bos taurus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
28000 | - |
2 * 28000, mitochondrial deoxyguanosine kinase, SDS-PAGE | Bos taurus |
56000 | - |
mitochondrial deoxyguanosine kinase, equilibrium sedimentation centrifugation | Bos taurus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
mitochondrial deoxyguanosine kinase, one-step purification via deoxyguanosine-3'-(4-aminophenylphosphate)-Sepharose affinity chromatography | Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Bos taurus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + deoxyguanosine | no activity with deoxycytidine | Bos taurus | ADP + dGMP | - |
? | |
ATP + deoxyguanosine | poor donor substrates are dATP or GTP | Bos taurus | ADP + dGMP | - |
? | |
ATP + deoxyguanosine | no activity with dGTP | Bos taurus | ADP + dGMP | - |
? | |
ATP + deoxyguanosine | ATP most efficient at pH 5.5, dTTP or UTP most efficient, i.e. more efficient than ATP, at pH 7.4 | Bos taurus | ADP + dGMP | - |
? | |
ATP + deoxyguanosine | no activity with dITP | Bos taurus | ADP + dGMP | - |
? | |
ATP + deoxyguanosine | no activity with adenosine | Bos taurus | ADP + dGMP | - |
? | |
ATP + deoxyguanosine | ATP can be replaced by dTTP, CTP, dCTP and UTP | Bos taurus | ADP + dGMP | - |
? | |
ATP + deoxyguanosine | highly specific for phospho group acceptor | Bos taurus | ADP + dGMP | - |
? | |
ATP + deoxyinosine | - |
Bos taurus | ADP + dIMP | - |
? | |
CTP + deoxyguanosine | - |
Bos taurus | CDP + dGMP | - |
? | |
dCTP + deoxyguanosine | - |
Bos taurus | dCDP + dGMP | - |
? | |
dTTP + deoxyguanosine | - |
Bos taurus | dTDP + dGMP | - |
? | |
UTP + deoxyguanosine | - |
Bos taurus | UDP + dGMP | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 28000, mitochondrial deoxyguanosine kinase, SDS-PAGE | Bos taurus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | 40 | - |
Bos taurus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
Triton X-100, 0.02%, stabilizes markedly against thermal inactivation | Bos taurus |
37 | - |
at least 4 h stable in the presence of Triton X-100, t1/2: 30 min at pH 11, inactivation within 30 min at pH 5.5 | Bos taurus |
40 | - |
denaturation above 40°C, mitochondrial deoxyguanosine kinase | Bos taurus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
- |
Bos taurus |
7.4 | - |
cosubstrates dTTP or UTP | Bos taurus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | - |
rapid decrease of activity below | Bos taurus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
95% loss of activity after 30 min at 37°C | Bos taurus |
7 | - |
most stable | Bos taurus |
11 | - |
50% loss of activity after 30 min at 37°C | Bos taurus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.075 | - |
deoxyinosine | pH 7.4, 37°C, competitive vs. deoxyguanosine, mitochondrial deoxyguanosine kinase | Bos taurus | |
0.117 | - |
1-alpha-D-arabinofuranosylguanine | pH 7.4, 37°C, competitive vs. deoxyguanosine, mitochondrial deoxyguanosine kinase | Bos taurus | |
0.43 | - |
8-aza-2'-deoxyguanosine | pH 7.4, 37°C, competitive vs. deoxyguanosine, mitochondrial deoxyguanosine kinase | Bos taurus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Bos taurus | - |
- |
5.9 |