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Literature summary for 2.7.1.113 extracted from

  • Barker, J.; Lewis, R.A.
    Deoxyguanosine kinase of neonatal mouse skin tissue (1981), Biochim. Biophys. Acta, 658, 111-123.
    View publication on PubMed

General Stability

General Stability Organism
ATP or MgATP2- stabilize, not Mg2+ alone Mus musculus
loss of activity in dilute solution Mus musculus

Inhibitors

Inhibitors Comment Organism Structure
deoxyadenosine 0.07 mM, 9% inhibition Mus musculus
deoxyinosine 0.07 mM, 17% inhibition Mus musculus
dGDP 0.1 mM, 97% inhibition Mus musculus
dGMP
-
Mus musculus
dGTP 0.1 mM, 99% inhibition Mus musculus
dTDP
-
Mus musculus
additional information not inhibited by deoxyadenosine Mus musculus
UDP strong inhibition Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.007
-
deoxyguanosine pH 5.2, 37°C Mus musculus
0.023
-
ATP pH 5.2, 37°C, at concentrations below 0.5 mM Mus musculus
0.023
-
ATP ATP in form of MgATP2- Mus musculus
0.63
-
ATP pH 5.2, 37°C, at concentrations above 0.5 mM Mus musculus
0.63
-
ATP ATP in form of MgATP2- Mus musculus

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
Mus musculus
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activation Mus musculus
Cd2+ slight activation Mus musculus
Mg2+ required for activity Mus musculus
Mn2+ can replace Mg2+ to some extent Mus musculus
Zn2+ activation, can replace Mg2+ to some extent Mus musculus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
44000
-
glycerol density gradient centrifugation Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + deoxyguanosine Mus musculus first reaction in reutilization of deoxyguanosine for dGTP biosynthesis ADP + dGMP
-
?

Organism

Organism UniProt Comment Textmining
Mus musculus
-
3 days old
-

Purification (Commentary)

Purification (Comment) Organism
pH 5.7, streptomycin sulfate, ammonium sulfate Mus musculus

Source Tissue

Source Tissue Comment Organism Textmining
skin neonatal skin tissue Mus musculus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0007
-
-
Mus musculus

Storage Stability

Storage Stability Organism
-20°C, ammonium sulfate precipitate in 100 mM M Tris-acetate buffer, pH 8, 0.025 M 2-mercaptoethanol, at least 4 months Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + deoxyguanosine no activity with deoxycytidine Mus musculus ADP + dGMP
-
?
ATP + deoxyguanosine no activity with dATP or GTP Mus musculus ADP + dGMP
-
?
ATP + deoxyguanosine no activity with deoxythymidine, uridine and cytosine Mus musculus ADP + dGMP
-
?
ATP + deoxyguanosine no activity with dGTP Mus musculus ADP + dGMP
-
?
ATP + deoxyguanosine no activity with adenosine Mus musculus ADP + dGMP
-
?
ATP + deoxyguanosine ATP can be replaced by dTTP, CTP, dCTP and UTP Mus musculus ADP + dGMP
-
?
ATP + deoxyguanosine 84%, 64%, 35% and 20% of activity with ATP with dTTP, CTP, dCTP and UTP, respectively Mus musculus ADP + dGMP
-
?
ATP + deoxyguanosine first reaction in reutilization of deoxyguanosine for dGTP biosynthesis Mus musculus ADP + dGMP
-
?
CTP + deoxyguanosine 64% of activity with ATP Mus musculus CDP + dGMP
-
?
dCTP + deoxyguanosine 35% of activity with ATP Mus musculus dCDP + dGMP
-
?
dTTP + deoxyguanosine 84% of activity with ATP Mus musculus dTDP + dGMP
-
?
UTP + deoxyguanosine 20% of activity with ATP Mus musculus UDP + dGMP
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.2
-
substrate deoxyguanosine Mus musculus

pH Range

pH Minimum pH Maximum Comment Organism
4.5 9 approx. 20% of maximal activity at pH 4.5 and pH 6.5 respectively, approx. 10% of maximal activity at pH 9.0 Mus musculus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00007
-
dGTP pH 5.2, 37°C, competitive vs. MgATP2-, at MgATP2- concentrations above 0.5 mM Mus musculus
0.0007
-
dGDP pH 5.2, 37°C, competitive vs. MgATP2-, at MgATP2- concentrations above 0.5 mM Mus musculus
0.0019
-
dGTP pH 5.2, 37°C, competitive vs. deoxyguanosine Mus musculus
0.003
-
UDP pH 5.2, 37°C, competitive vs. MgATP2-, at MgATP2- concentrations above 0.5 mM Mus musculus
0.18
-
dGMP pH 5.2, 37°C, competitive vs. deoxyguanosine Mus musculus