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Literature summary for 2.7.1.11 extracted from

  • Papagianni, M.; Legisa, M.
    Increased mannitol production in Lactobacillus reuteri ATCC 55730 production strain with a modified 6-phosphofructo-1-kinase (2014), J. Biotechnol., 181, 20-26.
    View publication on PubMed

Application

Application Comment Organism
synthesis expression of a truncated version of the gene encoding 6-phospho-1-fructokinase (tpfkA) along with its activator pkaC in Lactobacillus reuteri. Growth of the transformants at elevated glucose concentrations in the presence of fructose results in improved assimilation of the provided carbohydrates and a significant increase in the overall fermentation productivities. At the highest tested levels of glucose and fructose (75 g/l each), the transformant strain shows a 4fold increase in 6-phosphofructo-1-kinase activity and a 2.3fold increase in the glycolytic flux. The mannitol yield is 56 g/l compared to 10 g/l in the parental strain, and the lactate yield is 21 g/l (3.5 g/l in the parental strain). A high NADH/NAD+ratio occurrs under increased glycolytic flux conditions and facilitates the efficient conversion of fructose to mannitol Aspergillus niger

Organism

Organism UniProt Comment Textmining
Aspergillus niger
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Aspergillus niger NRRL 2270
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