Literature summary for 2.7.1.11 extracted from

Mediavilla, D.; Meton, I.; Baanante, I.V.
Purification and kinetic characterization of 6-phosphofructo-1-kinase from the liver of gilthead sea bream (Sparus aurata) (2008), J. Biochem., 144, 235-244.

Search for more literature for 2.7.1.11

Activating Compound

Activating Compound	Comment	Organism	Structure
D-fructose 2,6-bisphosphate	converts saturation curves for D-fructose 6-phosphate to hyperbolic and activates PFK synergistically with AMP	Sparus aurata

Inhibitors

Inhibitors	Comment	Organism	Structure
3-phosphoglycerate	about 70% inhibition at 7 mM	Sparus aurata
ADP	-	Sparus aurata
ATP	complete inhibition at 10 mM	Sparus aurata
citrate	almost complete inhibition at 1 mM citrate in the absence of D-fructose 2,6-bisphosphate	Sparus aurata
additional information	not inhibited by 6-phosphogluconate and phosphocreatine	Sparus aurata
phosphoenolpyruvate	about 30% inhibition at 5 mM	Sparus aurata

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.051	-	ATP	purified enzyme, at pH 7.0, in the absence of D-fructose 2,6-bisphosphate	Sparus aurata
0.076	-	ATP	purified enzyme, at pH 7.0, in the presence of 0.005 mM D-fructose 2,6-bisphosphate	Sparus aurata

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
86000	-	6 * 86000, apparent molecular weight estimated from SDS-PAGE	Sparus aurata
510000	-	native enzyme, gel filtration	Sparus aurata

Organism

Organism	UniProt	Comment	Textmining
Sparus aurata	-	gilthead sea bream	-

Purification (Commentary)

Purification (Comment)	Organism
12% PEG6000 precipitation, ATP agarose column chromatography, DEAE-Sepharose column chromatography, phosphocellulose column chromatography, and Blue-Sepharose column chromatography	Sparus aurata

Source Tissue

Source Tissue	Comment	Organism	Textmining
liver	-	Sparus aurata	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.04	-	crude extract	Sparus aurata
627	-	after 15700fold purification	Sparus aurata

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + D-fructose 6-phosphate	-	Sparus aurata	ADP + D-fructose 1,6-bisphosphate	-	?

Subunits

Subunits	Comment	Organism
homohexamer	6 * 86000, apparent molecular weight estimated from SDS-PAGE	Sparus aurata

Synonyms

Synonyms	Comment	Organism
6-phosphofructo-1-kinase	-	Sparus aurata
PFK	-	Sparus aurata

Cofactor

Cofactor	Comment	Organism	Structure
ATP	-	Sparus aurata

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Release 2023.1 (January 2023)