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Literature summary for 2.7.1.11 extracted from
- Identification of residues of Escherichia coli phosphofructokinase that contribute to nucleotide binding and specificity (1999), Biochemistry, 38, 4313-4318.
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| F76A | 62fold increase in Km for ATP | Escherichia coli |
| F76W | apparant Kms for ATP, ITP, GTP, CTP and UTP | Escherichia coli |
| F76Y | apparant Kms for ATP, ITP, GTP, CTP and UTP | Escherichia coli |
| R111E | apparant Kms for ATP, ITP, GTP, CTP and UTP | Escherichia coli |
| R11A | apparant Kms for ATP, ITP, GTP, CTP and UTP | Escherichia coli |
| R77A | apparant Kms for ATP, ITP, GTP, CTP and UTP | Escherichia coli |
| R77D | apparant Kms for ATP, ITP, GTP, CTP and UTP | Escherichia coli |
| R77E | apparant Kms for ATP, ITP, GTP, CTP and UTP | Escherichia coli |
| R77L | apparant Kms for ATP, ITP, GTP, CTP and UTP | Escherichia coli |
| R82A | 20fold increase in Km for ATP | Escherichia coli |
| R82E | apparant Kms for ATP, ITP, GTP, CTP and UTP | Escherichia coli |
| Y41A | 49fold increase in Km for ATP | Escherichia coli |
| Y41F | apparant Kms for ATP, ITP, GTP, CTP and UTP | Escherichia coli |
| Y41L | apparant Kms for ATP, ITP, GTP, CTP and UTP | Escherichia coli |
| Y41W | apparant Kms for ATP, ITP, GTP, CTP and UTP | Escherichia coli |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.21 | - |
ATP | pH 7.2, 30°C | Escherichia coli |  |
| 2.2 | - |
ITP | pH 7.2, 30°C | Escherichia coli | |
| 4.3 | - |
GTP | pH 7.2, 30°C | Escherichia coli | |
| 5.1 | - |
UTP | pH 7.2, 30°C | Escherichia coli | |
| 11 | - |
CTP | pH 7.2, 30°C | Escherichia coli | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | P0A796 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| CTP + D-fructose 6-phosphate | - |
Escherichia coli | CDP + D-fructose 1,6-bisphosphate | - |
? | |
| GTP + D-fructose 6-phosphate | - |
Escherichia coli | GDP + D-fructose 1,6-bisphosphate | - |
? | |
| ITP + D-fructose 6-phosphate | - |
Escherichia coli | IDP + D-fructose 1,6-bisphosphate | - |
? | |
| UTP + D-fructose 6-phosphate | - |
Escherichia coli | UDP + D-fructose 1,6-bisphosphate | - |
? | |
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