Protein Variants | Comment | Organism |
---|---|---|
E222A/H223A | ability to be allosterically regulated is retained | Escherichia coli |
H249E | ability to be allosterically regulated is retained, inhibition by phosphoenol pyruvate is lost | Escherichia coli |
M169A | ability to be allosterically regulated is retained | Escherichia coli |
R162E | ability to be allosterically regulated is retained | Escherichia coli |
R171E | approx. 6% of wild-type catalytic activity | Escherichia coli |
R243E | ability to be allosterically regulated is retained | Escherichia coli |
R252E | allosteric response to MgATP2- is lost, inhibition by phosphoenol pyruvate is lost | Escherichia coli |
R72E | approx. 0.5% of wild-type catalytic activity | Escherichia coli |
T125A | almost no activity detectable | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.015 | - |
fructose 6-phosphate | pH 8.0, 8.5°C, T125A mutant PFK | Escherichia coli | |
0.303 | - |
fructose 6-phosphate | pH 8.0, 8.5°C, R72E mutant PFK | Escherichia coli | |
3.47 | - |
fructose 6-phosphate | pH 8.0, 8.5°C, R171E mutant PFK | Escherichia coli | |
61 | - |
fructose 6-phosphate | pH 8.0, 8.5°C, wild-type PFK | Escherichia coli |