Crystallization (Comment) | Organism |
---|---|
hanging-drop vapour-diffusion, well solution consists of 1.7-1.8 M sodium formate, 100 mM sodium acetate, pH 4.6-4.8, hanging drop contains 4-5 mg/ml protein, 20 mM TEA buffer, pH 8.0, PFK is crystallized in the absence and presence of reaction products and the active site inhibitor 2,5-anhydro-1-deoxy-1-(m-nitro-D-mannitol-6-disodiumphosphate) at 2.9-4.0 A resolution | Trypanosoma brucei |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55585 | - |
4 * 55585, recombinant PFK, MALDI-TOF mass spectrometry | Trypanosoma brucei |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trypanosoma brucei | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant PFK, metal-affinity chromatography | Trypanosoma brucei |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 55585, recombinant PFK, MALDI-TOF mass spectrometry | Trypanosoma brucei |