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Literature summary for 2.7.1.11 extracted from

  • Hansen, T.; Schonheit, P.
    Purification and properties of the first-identified, archaeal, ATP-dependent 6-phosphofructokinase, an extremely thermophilic non-allosteric enzyme, from the hyperthermophile Desulfurococcus amylolyticus (2000), Arch. Microbiol., 173, 103-109.
    View publication on PubMed
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KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.1
-
 ATP pH 6.0, 50°C Desulfurococcus amylolyticus
0.28
-
 ATP pH 6.0, 85°C Desulfurococcus amylolyticus
0.49
-
 ADP pH 6.0, 85°C Desulfurococcus amylolyticus
0.57
-
 D-fructose 6-phosphate pH 6.0, 50°C Desulfurococcus amylolyticus
1.17
-
 D-fructose 6-phosphate pH 6.0, 85°C Desulfurococcus amylolyticus
16.7
-
 D-fructose 1,6-bisphosphate pH 6.0, 85°C Desulfurococcus amylolyticus

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ 12% of activity with Mg+ Desulfurococcus amylolyticus
Mg2+ required for activity Desulfurococcus amylolyticus
Mg2+ can be partially replaced by Ni2+, Mn2+ and Co2+ Desulfurococcus amylolyticus
Mn2+ 24% of activity with Mg2+ Desulfurococcus amylolyticus
Ni2+ 65% of activity with Mg2+ Desulfurococcus amylolyticus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33000
-
 alpha4, 4 * 33000, SDS-PAGE Desulfurococcus amylolyticus
140000
-
 gel filtration Desulfurococcus amylolyticus

Organism

Organism UniProt Comment Textmining
Desulfurococcus amylolyticus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
DEAE-Sepharose, ATP-agarose, Resource S Desulfurococcus amylolyticus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
47
-
 at 85°C Desulfurococcus amylolyticus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + D-fructose 6-phosphate reversible reaction in vivo, but not in vivo, very low activity with acetyl phosphate, UDP and GDP as phosphoryl donors, enzyme is not regulated allosterically Desulfurococcus amylolyticus ADP + D-fructose 1,6-bisphosphate
-
 r
CTP + D-fructose 6-phosphate 55% of activity with ATP Desulfurococcus amylolyticus CDP + D-fructose 1,6-bisphosphate
-
 ?
GTP + D-fructose 6-phosphate
-
 Desulfurococcus amylolyticus GDP + D-fructose 1,6-bisphosphate
-
 ?
ITP + D-fructose 6-phosphate 130% of activity with ATP Desulfurococcus amylolyticus IDP + D-fructose 1,6-bisphosphate
-
 ?
UTP + D-fructose 6-phosphate
-
 Desulfurococcus amylolyticus UDP + D-fructose 1,6-bisphosphate
-
 ?

Subunits

Subunits Comment Organism
tetramer alpha4, 4 * 33000, SDS-PAGE Desulfurococcus amylolyticus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
90
-
-
 Desulfurococcus amylolyticus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 100 very low activity at 40°C, approx. 50% of maximal activity at 70°C, activation energy: 42kJ/mol Desulfurococcus amylolyticus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
95
-
 50% loss of activity after 10 min, addition of 5 mM ADP and 25 mM MgCl2 increases the stability by up to 10°C Desulfurococcus amylolyticus
100
-
 approx. 70% loss of activity after 10 min in the absence of substrates Desulfurococcus amylolyticus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
 Desulfurococcus amylolyticus

pH Range

pH Minimum pH Maximum Comment Organism
4.5 6 30% of maximal activity at pH 4.5, 80% at pH 6.0 Desulfurococcus amylolyticus

pI Value

Organism Comment pI Value Maximum pI Value
Desulfurococcus amylolyticus chromatofocusing
-
 6