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Literature summary for 2.7.1.11 extracted from
- Phosphofructokinases from Lactobacteriaceae. II. Purification and properties of phosphofructokinase from Streptococcus thermophilus (1981), Biochim. Biophys. Acta, 661, 158-163.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | not inhibited by 2-phosphoglycerate; not inhibited by 3-phosphoglycerate; not inhibited by ADP; not inhibited by fructose 1,6-bisphosphate | Lactiplantibacillus plantarum | |
| additional information | not inhibited by 2-phosphoglycerate; not inhibited by 3-phosphoglycerate; not inhibited by ADP; not inhibited by fructose 1,6-bisphosphate | Lactobacillus acidophilus | |
| additional information | not inhibited by 2-phosphoglycerate; not inhibited by 3-phosphoglycerate; not inhibited by ADP; not inhibited by fructose 1,6-bisphosphate | Streptococcus thermophilus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.22 | 0.28 | ATP | - |
Streptococcus thermophilus |  |
| 0.23 | 0.28 | ATP | pH 7.0, 25°, 37°C and 50°C | Streptococcus thermophilus | |
| 1.44 | - |
D-fructose 6-phosphate | pH 7.0, 25°C | Streptococcus thermophilus | |
| 2.15 | - |
D-fructose 6-phosphate | pH 7.0, 37°C | Streptococcus thermophilus | |
| 3.44 | - |
D-fructose 6-phosphate | pH 7.0, 50°C | Streptococcus thermophilus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required for activity | Lactiplantibacillus plantarum | |
| Mg2+ | required for activity | Streptococcus thermophilus | |
| Mg2+ | required for activity | Lactobacillus acidophilus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Lactiplantibacillus plantarum | - |
- |
- |
| Lactobacillus acidophilus | - |
- |
- |
| Streptococcus thermophilus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| protamine sulfate, Cibacron blue Sepharose, DEAE-Sephadex | Streptococcus thermophilus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 134 | - |
- |
Streptococcus thermophilus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + D-fructose 6-phosphate | - |
Lactiplantibacillus plantarum | ADP + D-fructose 1,6-bisphosphate | - |
? | |
| ATP + D-fructose 6-phosphate | - |
Streptococcus thermophilus | ADP + D-fructose 1,6-bisphosphate | - |
? | |
| ATP + D-fructose 6-phosphate | - |
Lactobacillus acidophilus | ADP + D-fructose 1,6-bisphosphate | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Lactiplantibacillus plantarum |
| 25 | - |
assay at | Streptococcus thermophilus |
| 25 | - |
assay at | Lactobacillus acidophilus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 58 | - |
t1/2: 3 min | Lactobacillus acidophilus |
| 66 | - |
3 min, complete inactivation | Lactobacillus acidophilus |
| 75 | - |
complete inactivation after 2 min | Lactobacillus acidophilus |
| 75 | - |
complete inactivation after 4 min | Lactiplantibacillus plantarum |
| 75 | - |
30% loss of activity after 15 min | Streptococcus thermophilus |
| 79 | - |
complete inactivation after 3 min | Lactiplantibacillus plantarum |
| 85 | - |
t1/2: 3 min | Streptococcus thermophilus |
| 90 | - |
complete inactivation after 3 min | Streptococcus thermophilus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Streptococcus thermophilus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 8.5 | approx. 60% of maximal activity at pH 5.5, approx. 75% of maximal activity at pH 8.5 | Streptococcus thermophilus |
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