Activating Compound | Comment | Organism | Structure |
---|---|---|---|
GDP | - |
Escherichia coli |
Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant PFK in Escherichia coli | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
I126A | 600fold lower affinity for fructose 6-phosphate than wild-type, modestly lower Kcat than wild-type, greater ATP inhibition at pH 6.0 and 7.0 than at pH 8.0 | Escherichia coli |
R72H | almost no PFK activity | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ATP | at alkaline pH; mechanism | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.055 | - |
ATP | pH 8.5, 30°C, wild-type PFK | Escherichia coli | |
0.055 | - |
ATP | pH 8.5, 30°C, I126A mutant PFK | Escherichia coli | |
0.094 | - |
ATP | pH 8.5, 30°C, R72H mutant PFK | Escherichia coli | |
0.11 | - |
ATP | pH 8.5, 30°C, wild-type PFK | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required for activity | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
containing overproducing plasmid pHE1007 | - |
Escherichia coli | - |
containing overproducing plasmid TG-1 | - |
Escherichia coli | - |
mutant strains R72H and I127A | - |
Escherichia coli DF1020 | - |
containing overproducing plasmid pHE1007 | - |
Escherichia coli DF1020 | - |
containing overproducing plasmid TG-1 | - |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-fructose 6-phosphate | - |
Escherichia coli | ADP + D-fructose 1,6-bisphosphate | - |
? | |
ATP + D-fructose 6-phosphate | - |
Escherichia coli DF1020 | ADP + D-fructose 1,6-bisphosphate | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.3 | - |
fructose 6-phosphate | pH 8.5, 30°C, R72H mutant PFK | Escherichia coli | |
57.2 | - |
fructose 6-phosphate | pH 8.5, 30°C, I126A mutant PFK | Escherichia coli | |
111 | - |
fructose 6-phosphate | pH 8.5, 30°C, wild-type PFK | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
pH-dependence of kinetic properties | Escherichia coli |