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Literature summary for 2.7.1.11 extracted from

  • Fordyce, A.M.; Moore, C.H.; Pritchard, G.G.
    Phosphofructokinase from Streptococcus lactis (1982), Methods Enzymol., 90, 77-82.
    View publication on PubMed
Search for more literature for 2.7.1.11

Activating Compound

Activating Compound Comment Organism Structure
ADP activation Lactococcus lactis
NH4+
-
 Lactococcus lactis

Crystallization (Commentary)

Crystallization (Comment) Organism
4 mg/ml PFK in 25 mM sodium phosphate buffer pH 7.0, is dialyzed at 4°C against 25 mM phosphate buffer, pH 7.0, containing 10 mM 2-mercaptoethanol, 1 mM MgSO4, 1 mM EDTA, 2 mM ATP, 2 mM FDP and 23% ammonium sulfate, crystals appear after 8-10 weeks Lactococcus lactis

General Stability

General Stability Organism
dilution inactivates Lactococcus lactis
fructose 6-phosphate, ATP, ADP, citrate, KCl or ammonium sulfate does not stabilize Lactococcus lactis
glycerol prevents inactivation of dilute cytosolic enzyme solution Lactococcus lactis
glycerol stabilizes Lactococcus lactis

Inhibitors

Inhibitors Comment Organism Structure
AMP 5 mM, 80% inhibition Lactococcus lactis
D-fructose 1,6-bisphosphate 5 mM, 25% inhibition Lactococcus lactis
Li+ weak Lactococcus lactis
Na+ weak Lactococcus lactis
phosphate 10 mM, 50% inhibition Lactococcus lactis
phosphoenolpyruvate 5 mM; 64% inhibition Lactococcus lactis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.18
-
 ATP pH 7.5 Lactococcus lactis
0.18
-
 ATP ATP in form of MgATP2- Lactococcus lactis
0.25
-
 D-fructose 6-phosphate pH 7.5 Lactococcus lactis

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ 50% of activity with Mg2+- Lactococcus lactis
K+ slight activation at low concentrations Lactococcus lactis
Mg2+ required for activity Lactococcus lactis
Mg2+ maximal activity at 2-5 mM Lactococcus lactis
Mn2+ activation Lactococcus lactis
Mn2+ 75% of activity with Mg2+ Lactococcus lactis
Ni2+ activation, 12% of activity with Lactococcus lactis
Zn2+ activation Lactococcus lactis
Zn2+ 35% of activity with Mg2+ Lactococcus lactis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
144500
-
 gel filtration Lactococcus lactis

Organism

Organism UniProt Comment Textmining
Lactococcus lactis
-
-
-
Lactococcus lactis C10
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Lactococcus lactis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
161
-
-
 Lactococcus lactis

Storage Stability

Storage Stability Organism
-12°C, Tris-glycerol buffer, pH 7.5, 50% v/v glycerol, up to 6 months, 10-20% loss of activity Lactococcus lactis
4°C, less stable at pH 6 and 8 than at neutral pH-values Lactococcus lactis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + D-fructose 6-phosphate
-
 Lactococcus lactis ADP + D-fructose 1,6-bisphosphate
-
 ?
ATP + D-fructose 6-phosphate
-
 Lactococcus lactis C10 ADP + D-fructose 1,6-bisphosphate
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2 7.8
-
 Lactococcus lactis