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Literature summary for 2.7.1.11 extracted from
- Phosphofructokinase in the liver fluke Fasciola hepatica. Purification and kinetic changes by phosphorylation (1986), J. Biol. Chem., 261, 4346-4351.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| ADP | activation | Fasciola hepatica |  |
| cAMP | activation | Fasciola hepatica | |
| cAMP-dependent protein kinase | activation by phosphorylation | Fasciola hepatica | |
| NH4+ | - |
Fasciola hepatica | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ATP | 1 mM, almost complete inhibition of native PFK, phosphorylated PFK is less sensitive to inhibition | Fasciola hepatica | |
| ATP | - |
Ovis aries | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetic study | Ovis aries | |
| additional information | - |
additional information | kinetic study | Fasciola hepatica | |
| additional information | - |
additional information | kinetic properties of phosphorylated enzyme | Ovis aries | |
| additional information | - |
additional information | kinetic properties of phosphorylated enzyme | Fasciola hepatica | |
| 1.8 | - |
D-fructose 6-phosphate | pH 8.0, phosphorylated PFK | Fasciola hepatica | |
| 7 | - |
D-fructose 6-phosphate | pH 8.0, native PFK | Fasciola hepatica | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required for activity | Ovis aries | |
| Mg2+ | required for activity | Fasciola hepatica | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Fasciola hepatica | - |
- |
- |
| Ovis aries | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| side-chain modification | in vitro phosphorylation with cAMP-dependent protein kinase, 0.1-0.2 mol phosphate/mol of protomer, phosphorylation increases activity of PFK and reduces sensitivity to ATP inhibition | Fasciola hepatica |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| DEAE-cellulose, ammonium sulfate, AMP-agarose | Fasciola hepatica |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | - |
Ovis aries | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, enhanced stability in ammonium sulfate suspension | Fasciola hepatica |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + D-fructose 6-phosphate | - |
Ovis aries | ADP + D-fructose 1,6-bisphosphate | - |
? | |
| ATP + D-fructose 6-phosphate | - |
Fasciola hepatica | ADP + D-fructose 1,6-bisphosphate | - |
? | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.5 | - |
ATP | pH 8.0, native PFK | Fasciola hepatica | |
| 1.4 | - |
ATP | pH 8.0, phosphorylated PFK | Fasciola hepatica | |
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