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Literature summary for 2.7.1.11 extracted from

  • Hausler, R.E.; Holtum, J.A.M.; Latzko, E.
    Cytosolic phosphofructokinase from spinach leaves: 2. The roles of magnesium and ATP (1989), Plant Physiol., 90, 1506-1512.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
ADP activation Spinacia oleracea

Inhibitors

Inhibitors Comment Organism Structure
ATP free form Spinacia oleracea
Ca2+ depending upon total Mg2+-concentration, kinetics, cytosolic isozyme Spinacia oleracea
Mg2+ free form, citrate reverses, independent of MgATP-concentration, kinetics, cytosolic isozyme Spinacia oleracea
additional information not inhibited by cAMP; not inhibited by UDP, UDPglucose Spinacia oleracea
nucleoside triphosphate free form, pH-dependent Spinacia oleracea

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Spinacia oleracea 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required for activity Spinacia oleracea
Mg2+ MgNTP2- is the active substrate Spinacia oleracea
Mg2+ MgATP is the active substrate Spinacia oleracea

Organism

Organism UniProt Comment Textmining
Spinacia oleracea
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + D-fructose 6-phosphate
-
Spinacia oleracea ADP + D-fructose 1,6-bisphosphate
-
?
CTP + D-fructose 6-phosphate
-
Spinacia oleracea CDP + D-fructose 1,6-bisphosphate
-
?
GTP + D-fructose 6-phosphate
-
Spinacia oleracea GDP + D-fructose 1,6-bisphosphate
-
?
ITP + D-fructose 6-phosphate
-
Spinacia oleracea IDP + D-fructose 1,6-bisphosphate
-
?
UTP + D-fructose 6-phosphate
-
Spinacia oleracea UDP + D-fructose 1,6-bisphosphate
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Spinacia oleracea