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Literature summary for 2.7.1.11 extracted from

  • Cawood, M.E.; Botha, F.C.; Small, J.G.C.
    Properties of the phosphofructokinase isoenzymes from germinating cucumber seeds (1988), J. Plant Physiol., 132, 204-209.
No PubMed abstract available
Search for more literature for 2.7.1.11

Activating Compound

Activating Compound Comment Organism Structure
NH4+ 40 mM, 50% activation at pH 8.0 and pH 7.2 Cucumis sativus

Inhibitors

Inhibitors Comment Organism Structure
2-phosphoglycerate 2 mM and 3.2 mM, 50% inhibition of cytosolic PFK at pH 8.0 and PH 7.2, respectively, 0.5 mM and 0.4 mM, 50% inhibition of plastid PFK at pH 8.0 and pH 7.2, respectively Cucumis sativus
3-phosphoglycerate
-
 Cucumis sativus
ADP most potent inhibitor of cytosolic isozyme, 0.4 mM and 4 mM, 50% inhibition of cytosolic and plastid PFK at pH 8.0 Cucumis sativus
ATP 2 mM, 50% inhibition of cytosolic PFK at pH 8.0, 5 mM, 50% inhibition of plastid PFK at pH 8.0 Cucumis sativus
GTP plastid, not cytosolic, isozyme Cucumis sativus
additional information cytosolic PFK is not inhibited by GTP; not inhibited by fructose 2,6-bisphosphate Cucumis sativus
nucleoside triphosphate
-
 Cucumis sativus
phosphoenolpyruvate 0.04 mM and 0.05 mM, 50% inhibition of plastid PFK at pH 8.0 and pH 7.2, respectively; most potent inhibitor Cucumis sativus
SO42- 20 mM, 50% inhibition of cytosolic and 77% inhibition of plastid PFK at pH 7.2, not at pH 8.0 Cucumis sativus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information pH-dependence of kinetic properties of cytosolic and plastid isozymes Cucumis sativus
0.0006
-
 GTP pH 7.2, 25°C, cytosolic PFK Cucumis sativus
0.0009
-
 GTP pH 8.0, 25°C, cytosolic PFK Cucumis sativus
0.003
-
 ITP pH 8.0, 25°C, pH 7.2, 25°C, cytosolic PFK Cucumis sativus
0.004
-
 ITP pH 7.2, 25°C, plastid PFK Cucumis sativus
0.005
-
 ATP pH 7.2, 25°C, cytosolic PFK Cucumis sativus
0.006
-
 ATP pH 7.2, 25°C, plastid PFK Cucumis sativus
0.007
-
 ATP pH 8.0, 25°C, cytosolic PFK Cucumis sativus
0.008
-
 GTP pH 7.2, 25°C, plastid PFK Cucumis sativus
0.009
-
 UTP pH 7.2, 25°C, cytosolic PFK Cucumis sativus
0.01
-
 UTP pH 8.0, 25°C, plastid PFK Cucumis sativus
0.011
-
 ITP pH 8.0, 25°C, plastid PFK Cucumis sativus
0.013
-
 GTP pH 8.0, 25°C, plastid PFK Cucumis sativus
0.014
-
 ATP pH 8.0, 25°C, plastid PFK Cucumis sativus
0.018
-
 CTP pH 8.0, 25°C, cytosolic PFK Cucumis sativus
0.018
-
 UTP pH 8.0, 25°C, cytosolic PFK Cucumis sativus
0.019
-
 CTP pH 7.2, 25°C, plastid PFK Cucumis sativus
0.019
-
 UTP pH 7.2, 25°C, plastid PFK Cucumis sativus
0.021
-
 CTP pH 7.2, 25°C, cytosolic PFK Cucumis sativus
0.033
-
 CTP pH 8.0, 25°C, plastid PFK Cucumis sativus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol cytosolic and plastidic isoenzyme Cucumis sativus 5829
-
plastid
-
 Cucumis sativus 9536
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required for activity Cucumis sativus

Organism

Organism UniProt Comment Textmining
Cucumis sativus
-
 cucumber, cv. Fletcher
-

Source Tissue

Source Tissue Comment Organism Textmining
seed germinating Cucumis sativus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
15
-
 cytosolic PFK Cucumis sativus
27
-
 plastid PFK Cucumis sativus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + D-fructose 6-phosphate no activity with diphosphate as phosphate donor Cucumis sativus ADP + D-fructose 1,6-bisphosphate
-
 ?
CTP + D-fructose 6-phosphate
-
 Cucumis sativus CDP + D-fructose 1,6-bisphosphate
-
 ?
GTP + D-fructose 6-phosphate
-
 Cucumis sativus GDP + D-fructose 1,6-bisphosphate
-
 ?
ITP + D-fructose 6-phosphate
-
 Cucumis sativus IDP + D-fructose 1,6-bisphosphate
-
 ?
UTP + D-fructose 6-phosphate
-
 Cucumis sativus UDP + D-fructose 1,6-bisphosphate
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Cucumis sativus