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Literature summary for 2.7.1.11 extracted from

  • Isaac, J.E.; Rhodes, M.J.C.
    Purification and properties of phosphofructokinase from fruits of Lycopersicon esculentum (1982), Phytochemistry, 21, 1553-1556.
No PubMed abstract available

General Stability

General Stability Organism
dithioerythritol stabilizes Solanum lycopersicum
MgATP2- or ATP and Mg2+ stabilize during purification Solanum lycopersicum
sensitive to freezing, complete inactivation after 24 h at -20°C at pH 7.5 Solanum lycopersicum

Inhibitors

Inhibitors Comment Organism Structure
Cl-
-
Solanum lycopersicum
NO2-
-
Solanum lycopersicum
NO3-
-
Solanum lycopersicum
SO42-
-
Solanum lycopersicum

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required for activity Solanum lycopersicum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
gel filtration at pH 8 Solanum lycopersicum
180000
-
major form, gel filtration at pH 7.5 or 8.0 Solanum lycopersicum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + D-fructose 6-phosphate Solanum lycopersicum allosteric enzyme, involved in regulation of glycolytic flux ADP + D-fructose 1,6-bisphosphate
-
?

Organism

Organism UniProt Comment Textmining
Solanum lycopersicum
-
tomato, var. Eurocross BB
-

Purification (Commentary)

Purification (Comment) Organism
Blue Sepharose, ATP-Sepharose Solanum lycopersicum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.61
-
-
Solanum lycopersicum

Storage Stability

Storage Stability Organism
-20°C, inactivation within 24 h Solanum lycopersicum
0-4°C, more stable in Tris than in phosphate buffer Solanum lycopersicum
enzyme extracts prepared at pH 8 are less stable to storage at 0-4°C than extracts prepared at pH 7.5 Solanum lycopersicum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + D-fructose 6-phosphate
-
Solanum lycopersicum ADP + D-fructose 1,6-bisphosphate
-
?
ATP + D-fructose 6-phosphate allosteric enzyme, involved in regulation of glycolytic flux Solanum lycopersicum ADP + D-fructose 1,6-bisphosphate
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Solanum lycopersicum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
3 min, inactivation, enzyme prepared by ATP-Sepharose chromatography Solanum lycopersicum
100
-
5 min, 70% loss of activity, enzyme prepared by DEAE-cellulose chromatography in the absence of MgATP Solanum lycopersicum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 8.3
-
Solanum lycopersicum

pH Range

pH Minimum pH Maximum Comment Organism
7.5 8.3 approx. half-maximal activity at pH 7.5, maximal activity at pH 8.3, approx. 15% of maximal activity at pH 7 Solanum lycopersicum