General Stability | Organism |
---|---|
dithioerythritol stabilizes | Solanum lycopersicum |
MgATP2- or ATP and Mg2+ stabilize during purification | Solanum lycopersicum |
sensitive to freezing, complete inactivation after 24 h at -20°C at pH 7.5 | Solanum lycopersicum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cl- | - |
Solanum lycopersicum | |
NO2- | - |
Solanum lycopersicum | |
NO3- | - |
Solanum lycopersicum | |
SO42- | - |
Solanum lycopersicum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required for activity | Solanum lycopersicum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
gel filtration at pH 8 | Solanum lycopersicum |
180000 | - |
major form, gel filtration at pH 7.5 or 8.0 | Solanum lycopersicum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-fructose 6-phosphate | Solanum lycopersicum | allosteric enzyme, involved in regulation of glycolytic flux | ADP + D-fructose 1,6-bisphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Solanum lycopersicum | - |
tomato, var. Eurocross BB | - |
Purification (Comment) | Organism |
---|---|
Blue Sepharose, ATP-Sepharose | Solanum lycopersicum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.61 | - |
- |
Solanum lycopersicum |
Storage Stability | Organism |
---|---|
-20°C, inactivation within 24 h | Solanum lycopersicum |
0-4°C, more stable in Tris than in phosphate buffer | Solanum lycopersicum |
enzyme extracts prepared at pH 8 are less stable to storage at 0-4°C than extracts prepared at pH 7.5 | Solanum lycopersicum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-fructose 6-phosphate | - |
Solanum lycopersicum | ADP + D-fructose 1,6-bisphosphate | - |
? | |
ATP + D-fructose 6-phosphate | allosteric enzyme, involved in regulation of glycolytic flux | Solanum lycopersicum | ADP + D-fructose 1,6-bisphosphate | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Solanum lycopersicum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
3 min, inactivation, enzyme prepared by ATP-Sepharose chromatography | Solanum lycopersicum |
100 | - |
5 min, 70% loss of activity, enzyme prepared by DEAE-cellulose chromatography in the absence of MgATP | Solanum lycopersicum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | 8.3 | - |
Solanum lycopersicum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.5 | 8.3 | approx. half-maximal activity at pH 7.5, maximal activity at pH 8.3, approx. 15% of maximal activity at pH 7 | Solanum lycopersicum |