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Literature summary for 2.7.1.11 extracted from

  • Kelly, G.J.; Mukherjee, U.; Holtum, J.A.M.; Latzko, E.
    Purification, kinetics and regulatory properties of phosphofructokinase from Chlorella pyrenoidosa (1985), Plant Cell Physiol., 26, 301-307.
No PubMed abstract available
Search for more literature for 2.7.1.11

General Stability

General Stability Organism
50 mM phosphate stabilizes at pH 6.6 Auxenochlorella pyrenoidosa

Inhibitors

Inhibitors Comment Organism Structure
ATP above 0.5 mM Auxenochlorella pyrenoidosa
phosphate inhibition at 10-100 mM, concentrations at 1- 5 mM relieve the influence of other inhibitors such as phosphoenolpyruvate Auxenochlorella pyrenoidosa
phosphoenolpyruvate 0.1 mM, 50% inhibition at low pH; phosphate relieves Auxenochlorella pyrenoidosa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.009
-
 ATP pH 7.7 Auxenochlorella pyrenoidosa
0.19 0.2 D-fructose 6-phosphate
-
 Auxenochlorella pyrenoidosa
0.2
-
 fructose 6-phosphate pH 7.7 Auxenochlorella pyrenoidosa

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast possibly located in chloroplast Auxenochlorella pyrenoidosa 9507
-
plastid chloroplasts Auxenochlorella pyrenoidosa 9536
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required for activity Auxenochlorella pyrenoidosa

Organism

Organism UniProt Comment Textmining
Auxenochlorella pyrenoidosa
-
 green alga
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate, Sepharose 4b, DEAE-Sephacel Auxenochlorella pyrenoidosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + D-fructose 6-phosphate
-
 Auxenochlorella pyrenoidosa ADP + D-fructose 1,6-bisphosphate
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.7
-
-
 Auxenochlorella pyrenoidosa