BRENDA - Enzyme Database
show all sequences of 2.7.1.108

Expression and characterization of a human cDNA that complements the temperature-sensitive defect in dolichol kinase activity in the yeast sec59-1 mutant: the enzymatic phosphorylation of dolichol and diacylglycerol are catalyzed by separate CTP-mediated kinase activities in Saccharomyces cerevisiae

Fernandez, F.; Shridas, P.; Jiang, S.; Aebi, M.; Waechter, C.J.; Glycobiology 12, 555-562 (2002)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
-
Saccharomyces cerevisiae
human cDNA complements defect in dolichol kinase activity in Saccharomyces cerevisiae sec59-1 mutant, human SEC59 gene also expressed in Sf-9 cells
Homo sapiens
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
-
Homo sapiens
16020
-
membrane
-
Saccharomyces cerevisiae
16020
-
microsome
-
Saccharomyces cerevisiae
-
-
microsome
-
Homo sapiens
-
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
59000
60000
SDS-PAGE
Homo sapiens
59270
-
predicted from cDNA open reading frame
Homo sapiens
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
CTP + dolichol
Homo sapiens
CTP mediated phosphorylation of dolichol, the terminal step in dolichyl monophospate biosynthesis de novo
CDP + dolichyl phosphate
-
Homo sapiens
?
CTP + dolichol
Saccharomyces cerevisiae
CTP mediated phosphorylation of dolichol, the terminal step in dolichyl monophospate biosynthesis de novo
CDP + dolichyl phosphate
-
Saccharomyces cerevisiae
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Homo sapiens
-
clone KIAA1094 from a human brain library
-
Saccharomyces cerevisiae
P20048
yeast, SEC59 gene
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
brain
-
Homo sapiens
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
CTP + dolichol
-
640401
Homo sapiens
CDP + dolichyl phosphate
-
-
-
?
CTP + dolichol
-
640401
Saccharomyces cerevisiae
CDP + dolichyl phosphate
-
-
-
?
CTP + dolichol
CTP mediated phosphorylation of dolichol, the terminal step in dolichyl monophospate biosynthesis de novo
640401
Homo sapiens
CDP + dolichyl phosphate
-
640401
Homo sapiens
?
CTP + dolichol
CTP mediated phosphorylation of dolichol, the terminal step in dolichyl monophospate biosynthesis de novo
640401
Saccharomyces cerevisiae
CDP + dolichyl phosphate
-
640401
Saccharomyces cerevisiae
?
Cloned(Commentary) (protein specific)
Commentary
Organism
-
Saccharomyces cerevisiae
human cDNA complements defect in dolichol kinase activity in Saccharomyces cerevisiae sec59-1 mutant, human SEC59 gene also expressed in Sf-9 cells
Homo sapiens
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
-
Homo sapiens
16020
-
membrane
-
Saccharomyces cerevisiae
16020
-
microsome
-
Homo sapiens
-
-
microsome
-
Saccharomyces cerevisiae
-
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
59000
60000
SDS-PAGE
Homo sapiens
59270
-
predicted from cDNA open reading frame
Homo sapiens
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
CTP + dolichol
Homo sapiens
CTP mediated phosphorylation of dolichol, the terminal step in dolichyl monophospate biosynthesis de novo
CDP + dolichyl phosphate
-
Homo sapiens
?
CTP + dolichol
Saccharomyces cerevisiae
CTP mediated phosphorylation of dolichol, the terminal step in dolichyl monophospate biosynthesis de novo
CDP + dolichyl phosphate
-
Saccharomyces cerevisiae
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
brain
-
Homo sapiens
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
CTP + dolichol
-
640401
Homo sapiens
CDP + dolichyl phosphate
-
-
-
?
CTP + dolichol
-
640401
Saccharomyces cerevisiae
CDP + dolichyl phosphate
-
-
-
?
CTP + dolichol
CTP mediated phosphorylation of dolichol, the terminal step in dolichyl monophospate biosynthesis de novo
640401
Homo sapiens
CDP + dolichyl phosphate
-
640401
Homo sapiens
?
CTP + dolichol
CTP mediated phosphorylation of dolichol, the terminal step in dolichyl monophospate biosynthesis de novo
640401
Saccharomyces cerevisiae
CDP + dolichyl phosphate
-
640401
Saccharomyces cerevisiae
?
Other publictions for EC 2.7.1.108
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
739314
Nakamura
Arabidopsis DOK1 encodes a fun ...
Arabidopsis thaliana
Plant J.
81
292-303
2015
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1
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1
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2
2
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739079
Lieu
Severe, fatal multisystem mani ...
Homo sapiens
Mol. Genet. Metab.
110
484-489
2013
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1
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1
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1
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3
3
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723805
Juchimiuk
Defect in dolichol-dependent g ...
Saccharomyces cerevisiae
Yeast
27
637-645
2010
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1
1
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696448
Denecke
Hypoglycosylation due to dolic ...
Homo sapiens
Biochim. Biophys. Acta
1792
888-895
2009
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2
2
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687738
Han
Characterization of the yeast ...
Saccharomyces cerevisiae
J. Biol. Chem.
283
20443-20453
2008
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4
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1
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687781
Rush
Recycling of dolichyl monophos ...
Bos taurus
J. Biol. Chem.
283
4087-4093
2008
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1
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671201
Kranz
A defect in dolichol phosphate ...
Homo sapiens
Am. J. Hum. Genet.
80
433-440
2007
-
-
-
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-
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2
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3
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1
1
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3
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677274
Orlowski
Dissecting the role of dolicho ...
Saccharomyces cerevisiae, Saccharomyces cerevisiae SF402-4D
Yeast
24
239-252
2007
-
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1
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1
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674673
Shridas
Human dolichol kinase, a polyt ...
Homo sapiens
J. Biol. Chem.
281
31696-31704
2006
-
-
1
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2
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5
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2
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2
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640401
Fernandez
Expression and characterizatio ...
Homo sapiens, Saccharomyces cerevisiae
Glycobiology
12
555-562
2002
-
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2
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4
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2
2
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4
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1
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4
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4
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2
2
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1
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4
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-
640400
Rymerson
Dolichol and polyprenol kinase ...
Secale cereale
Biochem. Cell Biol.
70
455-459
1992
1
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1
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5
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1
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640399
Itami
Dolichol kinase in rat sarcopl ...
Rattus norvegicus, Rattus norvegicus Sprague-Dawley
Biochim. Biophys. Acta
960
374-381
1988
1
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2
2
4
4
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2
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163
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6
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1
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2
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6
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4
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1
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640398
Ravi
-
Characterization of dolichol k ...
Glycine max
Biochim. Biophys. Acta
875
618-625
1986
1
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-
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-
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2
1
2
1
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1
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1
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1
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1
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1
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1
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2
1
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640395
Sakakihara
Zn2+, not Ca2+, is the most ef ...
Bos taurus, Rattus norvegicus, Rattus norvegicus Sprague-Dawley
J. Biol. Chem.
260
15413-15419
1985
-
-
-
-
-
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2
2
2
14
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3
-
164
-
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2
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3
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2
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14
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3
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2
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3
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640396
Sumbilla
Properties of brain dolichol k ...
Bos taurus
Arch. Biochem. Biophys.
238
75-82
1985
4
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4
2
1
2
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1
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1
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2
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1
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640397
Walter
Dolichol kinase in Ascaris suu ...
Ascaris suum, Onchocerca volvulus
Mol. Biochem. Parasitol.
14
211-217
1985
2
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4
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6
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2
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640394
Gandhi
Calmodulin-dependent enzymic p ...
Tetrahymena pyriformis
Biochem. J.
216
317-323
1983
1
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1
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640392
Allen
Extraction and detergent/lipid ...
Bos taurus
Biochim. Biophys. Acta
712
33-41
1982
3
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5
2
2
4
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1
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1
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1
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1
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1
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1
1
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640393
Keller
Subcellular localization and s ...
Rattus norvegicus, Rattus norvegicus Sprague-Dawley
Biochim. Biophys. Acta
719
118-125
1982
-
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7
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2
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164
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1
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4
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10
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7
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2
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1
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4
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10
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640391
Rip
Properties of a dolichol phosp ...
Rattus norvegicus, Rattus norvegicus Wistar
Can. J. Biochem.
58
1051-1057
1980
2
-
-
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4
2
1
3
-
2
-
167
-
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2
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4
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1
1
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1
3
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2
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2
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4
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1
1
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640390
Burton
Enzymatic phosphorylation of d ...
Bos taurus
J. Biol. Chem.
254
7129-7136
1979
2
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1
1
1
3
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1
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1
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1
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3
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1
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