Literature summary for 2.7.1.108 extracted from

Gandhi, C.R.; Keenan, R.W.
Calmodulin-dependent enzymic phosphorylation of dolichol in Tetrahymena pyriformis (1983), Biochem. J., 216, 317-323.

Search for more literature for 2.7.1.108

Activating Compound

Activating Compound	Comment	Organism	Structure
Triton X-100	-	Tetrahymena pyriformis

Inhibitors

Inhibitors	Comment	Organism	Structure
chlorpromazine	-	Tetrahymena pyriformis
Trifluoperazine	-	Tetrahymena pyriformis

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.07	-	dolichol	pH 7.4, 37°C	Tetrahymena pyriformis
3	-	CTP	pH 7.4, 37°C	Tetrahymena pyriformis

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Ca2+	required for maxiumum activity	Tetrahymena pyriformis
Mg2+	required for maxiumum activity	Tetrahymena pyriformis
Mn2+	activated by	Tetrahymena pyriformis

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
CTP + dolichol	Tetrahymena pyriformis	-	CDP + dolichyl phosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Tetrahymena pyriformis	-	-	-

Storage Stability

Storage Stability	Organism
-20°C, stable for at least 15 days	Tetrahymena pyriformis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
CTP + dolichol	-	Tetrahymena pyriformis	CDP + dolichyl phosphate	-	?
additional information	ATP, UTP and GTP are ineffective	Tetrahymena pyriformis	?	-	?

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.4	-	Tris/maleate buffer, second optimum at pH 9.0	Tetrahymena pyriformis
9	-	glycine buffer, second optimum at pH 7.4	Tetrahymena pyriformis

pH Range

pH Minimum	pH Maximum	Comment	Organism
6.6	9.6	2 optima, about half-maximal activity at pH 6.6, pH 8.5 and pH 9.6	Tetrahymena pyriformis

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)