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Literature summary for 2.7.1.108 extracted from
- Extraction and detergent/lipid activation of dolichol kinase (1982), Biochim. Biophys. Acta, 712, 33-41.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| dimyristoylphosphatidylcholine | stimulatory in 1:1 ratio with deoxycholate | Bos taurus |  |
| additional information | sodium dodecylsulfate is ineffective in stimulating enzymic activity | Bos taurus | |
| Triton X-100 | - |
Bos taurus | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | above 6 mM | Bos taurus | |
| Co2+ | above 6 mM | Bos taurus | |
| Mn2+ | above 6 mM | Bos taurus | |
| additional information | Mg2+ is not inhibitory | Bos taurus | |
| Zn2+ | - |
Bos taurus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.15 | - |
CTP | pH 7.5, 35°C, in deoxycholate/dimyristoylphosphatidylcholine 1:1 | Bos taurus | |
| 0.55 | - |
CTP | pH 7.5, 35°C, in Triton X-100/dimyristoylphosphatidylcholine 1:1 | Bos taurus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | - |
Bos taurus | 16020 | - |
| microsome | - |
Bos taurus | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | - |
Bos taurus | |
| Co2+ | divalent cation required for activity, Mg2+, Ca2+ and Co2+ are equally effective | Bos taurus | |
| Mg2+ | enzyme is dependent on divalent cations | Bos taurus | |
| additional information | Zn2+ is not effective | Bos taurus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| CTP + dolichol | Bos taurus | - |
CDP + dolichyl phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
bovine | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partially | Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Bos taurus | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, stored frozen as pellet for months with little loss of activity | Bos taurus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| CTP + dolichol | - |
Bos taurus | CDP + dolichyl phosphate | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
- |
Bos taurus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.6 | 10.2 | broad pH-activity profile with good activity observed from pH 7.0-10.0, about half-maximal activity at pH 6.6 and 10.2 | Bos taurus |
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Release 2023.1 (January 2023)
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