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Literature summary for 2.7.1.107 extracted from

  • Natalini, P.M.; Zulian, S.E.; Ilincheta de Boschero, M.G.; Giusto, N.M.
    Diacylglycerol kinase epsilon in bovine and rat photoreceptor cells. Light-dependent distribution in photoreceptor cells (2013), Exp. Eye Res., 112, 139-150.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
additional information no inhibition of activity of isozyme DAGKepsilon with 1-stearoyl-2-arachidonoylglycerol by R59022, a type I DAGK inhibitor Bos taurus
additional information no inhibition by R59022, a type I DAGK inhibitor Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
photoreceptor outer segment from albino rats, isozyme DAGKepsilon appears to be higher in rod outer segments from rat retinas exposed to light than in those under dark conditions. DAGKepsilon in rat retina naturally exposed to room light is present in all retinal layers and is distributed along the photoreceptor cell Rattus norvegicus 1750
-
photoreceptor outer segment in bovine rod outer segments obtained from retinas under room light conditions, DAGKepsilon and type I DAGK activity is present. Isozyme DAGKepsilon appears to be higher in rod outer segments from bleached bovine retinas than in those under dark conditions Bos taurus 1750
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Rattus norvegicus
Mg2+ required Bos taurus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + 1,2-diacyl-sn-glycerol Rattus norvegicus
-
ADP + 1,2-diacyl-sn-glycerol 3-phosphate
-
?
ATP + 1,2-diacyl-sn-glycerol Bos taurus
-
ADP + 1,2-diacyl-sn-glycerol 3-phosphate
-
?
ATP + 1,2-diacyl-sn-glycerol Rattus norvegicus Wistar
-
ADP + 1,2-diacyl-sn-glycerol 3-phosphate
-
?

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-
Rattus norvegicus
-
-
-
Rattus norvegicus Wistar
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
additional information light-dependent outer segment localization of DAGKepsilon, DAGKepsilon distribution in the photoreceptor cell is dependent on PIP2-PLC activation by light Rattus norvegicus
-
additional information light-dependent outer segment localization of DAGKepsilon, DAGKepsilon distribution in the photoreceptor cell is dependent on PIP2-PLC activation by light Bos taurus
-
photoreceptor cell the selective distribution of isozyme DAGKepsilon in photoreceptor cells is a light-dependent mechanism that promotes increased 1-stearoyl, 2-arachidonoylglycerol removal and synthesis of 1-stearoyl, 2-arachidonoyl phosphatidic acid in the sensorial portion of this cell Rattus norvegicus
-
photoreceptor cell the selective distribution of isozyme DAGKepsilon in photoreceptor cells is a light-dependent mechanism that promotes increased 1-stearoyl, 2-arachidonoylglycerol removal and synthesis of 1-stearoyl, 2-arachidonoyl phosphatidic acid in the sensorial portion of this cell Bos taurus
-
retina
-
Rattus norvegicus
-
retina
-
Bos taurus
-
retinal rod from albino rats, isozyme DAGKepsilon appears to be higher in rod outer segments from rat retinas exposed to light than in those under dark conditions. DAGKepsilon in rat retina naturally exposed to room light is present in all retinal layers and is distributed along the photoreceptor cell Rattus norvegicus
-
retinal rod in bovine rod outer segments obtained from retinas under room light conditions, DAGKepsilon and type I DAGK activity is present. Isozyme DAGKepsilon appears to be higher in rod outer segments from bleached bovine retinas than in those under dark conditions Bos taurus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 1,2-diacyl-sn-glycerol
-
Rattus norvegicus ADP + 1,2-diacyl-sn-glycerol 3-phosphate
-
?
ATP + 1,2-diacyl-sn-glycerol
-
Bos taurus ADP + 1,2-diacyl-sn-glycerol 3-phosphate
-
?
ATP + 1,2-diacyl-sn-glycerol
-
Rattus norvegicus Wistar ADP + 1,2-diacyl-sn-glycerol 3-phosphate
-
?
ATP + 1,2-dioleoylglycerol low activity Bos taurus ADP + 1,2-dioleoylglycerol 3-phospate
-
?
ATP + 1-stearoyl-2-arachidonoylglycerol preferred enzyme, 6fold higher activity compared to substrate 1,2-dioleoylglycerol Bos taurus ADP + 1-stearoyl-2-arachidonoylglycerol 3-phosphate
-
?
additional information enzyme assays with isolated rod outer segments prepared under room light from retinas obtained from dark-adapted bovine eyes Bos taurus ?
-
?

Synonyms

Synonyms Comment Organism
1,2-diacylglycerol kinase epsilon
-
Rattus norvegicus
1,2-diacylglycerol kinase epsilon
-
Bos taurus
DAGK
-
Rattus norvegicus
DAGK
-
Bos taurus
DAGKepsilon
-
Rattus norvegicus
DAGKepsilon
-
Bos taurus
diacylglycerol kinase epsilon
-
Rattus norvegicus
diacylglycerol kinase epsilon
-
Bos taurus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Bos taurus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
assay at Rattus norvegicus
7.4
-
assay at Bos taurus

Cofactor

Cofactor Comment Organism Structure
ATP
-
Rattus norvegicus
ATP
-
Bos taurus

General Information

General Information Comment Organism
physiological function the enzyme is involved in a mechanism of light-regulated DAGK activity in the photoreceptors of the vertebrate species. Protein kinase C-dependent phosphorylation of retina rod outer segments (obtained from bovine retinas at room light) regulates DAGK activity Bos taurus
physiological function the enzyme is involved in a mechanism of light-regulated DAGK activity in the photoreceptors of the vertebrate species. Protein kinase C-dependent phosphorylation of retina rod outer segments regulates DAGK activity Rattus norvegicus