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Literature summary for 2.7.1.107 extracted from

  • Sato, Y.; Murakami, C.; Yamaki, A.; Mizuno, S.; Sakai, H.; Sakane, F.
    Distinct 1-monoacylglycerol and 2-monoacylglycerol kinase activities of diacylglycerol kinase isozymes (2016), Biochim. Biophys. Acta, 1864, 1170-1176.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression of human His6-tagged DGK isozyme theta from vector pSF-CMV-NH2-His-EKT3 in COS-7 cells Homo sapiens
recombinant expression of human p3×FLAG-CMV-DGK isozyme delta2 in COS-7 cells Homo sapiens
recombinant expression of human p3×FLAG-CMV-DGK isozyme epsilon in COS-7 cells Homo sapiens
recombinant expression of human p3×FLAG-CMV-DGK isozyme eta1 in COS-7 cells Homo sapiens
recombinant expression of human p3×FLAG-CMV-DGK isozyme gamma in COS-7 cells Homo sapiens
recombinant expression of human p3×FLAG-CMV-DGK isozyme iota1 in COS-7 cells Homo sapiens
recombinant expression of human p3×FLAG-CMV-DGK isozyme kappa in COS-7 cells Homo sapiens
recombinant expression of human p3×FLAG-CMV-DGK isozyme zeta1 in COS-7 cells Homo sapiens
recombinant expression of pig p3×FLAG-CMV-DGK isozyme alpha in COS-7 cells Sus scrofa
recombinant expression of rat p3×FLAG-CMV-DGK isozyme beta in COS-7 cells Rattus norvegicus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Homo sapiens
Mg2+ required Sus scrofa
Mg2+ required Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Homo sapiens O75912 isozyme iota1
-
Homo sapiens P49619 isozyme gamma; isozyme gamma
-
Homo sapiens P52429 isozyme epsilon; isozyme epsilon
-
Homo sapiens P52824 isozyme theta
-
Homo sapiens Q13574 isozyme zeta1
-
Homo sapiens Q16760 isozyme delta2; isozyme delta2
-
Homo sapiens Q5KSL6 isozyme kappa; isozyme kappa
-
Homo sapiens Q86XP1 isozyme eta1; isozyme eta1
-
Rattus norvegicus P68403 isozyme beta; isozyme beta
-
Sus scrofa P20192 isozyme alpha; isozyme alpha
-

Purification (Commentary)

Purification (Comment) Organism
recombinant human His6-tagged DGK isozyme theta from COS-7 cells by nickel affinity chromatography Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme also shows 1-monoacylglycerol kinase (1-MGK) and low 2-monoacylglycerol kinase (2-MGK) activities, cf. EC 2.7.1.94 Homo sapiens ?
-
?
additional information the enzyme also shows 1-monoacylglycerol kinase (1-MGK) and low 2-monoacylglycerol kinase (2-MGK) activities, cf. EC 2.7.1.94 Sus scrofa ?
-
?
additional information the enzyme also shows 1-monoacylglycerol kinase (1-MGK) and low 2-monoacylglycerol kinase (2-MGK) activities, cf. EC 2.7.1.94 Rattus norvegicus ?
-
?
additional information the enzyme also shows low 1-monoacylglycerol kinase (1-MGK) and low 2-monoacylglycerol kinase (2-MGK) activities, cf. EC 2.7.1.94 Homo sapiens ?
-
?
additional information the enzyme also shows negligible 1-monoacylglycerol kinase (1-MGK) and 2-monoacylglycerol kinase (2-MGK) activities, cf. EC 2.7.1.94 Homo sapiens ?
-
?
additional information the enzyme also shows negligible 1-monoacylglycerol kinase (1-MGK) and low 2-monoacylglycerol kinase (2-MGK) activities, cf. EC 2.7.1.94 Homo sapiens ?
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Homo sapiens
30
-
assay at Sus scrofa
30
-
assay at Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
assay at Homo sapiens
7.2
-
assay at Sus scrofa
7.2
-
assay at Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
ATP
-
Homo sapiens
ATP
-
Sus scrofa
ATP
-
Rattus norvegicus

General Information

General Information Comment Organism
evolution comprehensive analysis on the 1-monoacylglycerol kinase (1-MGK) and 2-monoacylglycerol kinase (2-MGK) activities, EC 2.7.1.94, of ten diacylglyceol kinase (DGK) isozymes, EC 2.7.1.107, from different organisms. Type I (alpha, beta, and gamma), type II (delta, eta, and kappa) and type III (epsilon) DGKs have 7.9-19.2% 2-MGK activity compared to their DGK activities, whereas their 1-MGK activities are below 3.0%. Both the 1-MGK and 2-MGK activities of the type IV DGKs (zeta and iota) are below 1% relative to their DGK activities. Type V DGKtheta has approximately 6% 1-MGK activity and below 2% 2-MGK activity compared to its DGK activity. Purified DGKtheta exhibits the same results, indicating that its 1-MGK activity is intrinsic. DGK isozymes are categorized into three types with respect to their 1-MGK and 2-MGK activities: those having (1) 2-MGK activity relatively stronger than their 1-MGK activity (types I-III), (2) only negligible 1-MGK and 2-MGK activities (type IV), and (3) 1-MGK activity stronger than its 2-MGK activity (type V). The 1-MGK activity of DGKtheta and the 2-MGK activity of DGKalpha are stronger than those of the acylglycerol kinase reported as 1-MGK and 2-MGK to date Homo sapiens
evolution comprehensive analysis on the 1-monoacylglycerol kinase (1-MGK) and 2-monoacylglycerol kinase (2-MGK) activities, EC 2.7.1.94, of ten diacylglyceol kinase (DGK) isozymes, EC 2.7.1.107, from different organisms. Type I (alpha, beta, and gamma), type II (delta, eta, and kappa) and type III (epsilon) DGKs have 7.9-19.2% 2-MGK activity compared to their DGK activities, whereas their 1-MGK activities are below 3.0%. Both the 1-MGK and 2-MGK activities of the type IV DGKs (zeta and iota) are below 1% relative to their DGK activities. Type V DGKtheta has approximately 6% 1-MGK activity and below 2% 2-MGK activity compared to its DGK activity. Purified DGKtheta exhibits the same results, indicating that its 1-MGK activity is intrinsic. DGK isozymes are categorized into three types with respect to their 1-MGK and 2-MGK activities: those having (1) 2-MGK activity relatively stronger than their 1-MGK activity (types I-III), (2) only negligible 1-MGK and 2-MGK activities (type IV), and (3) 1-MGK activity stronger than its 2-MGK activity (type V). The 1-MGK activity of DGKtheta and the 2-MGK activity of DGKalpha are stronger than those of the acylglycerol kinase reported as 1-MGK and 2-MGK to date Sus scrofa
evolution comprehensive analysis on the 1-monoacylglycerol kinase (1-MGK) and 2-monoacylglycerol kinase (2-MGK) activities, EC 2.7.1.94, of ten diacylglyceol kinase (DGK) isozymes, EC 2.7.1.107, from different organisms. Type I (alpha, beta, and gamma), type II (delta, eta, and kappa) and type III (epsilon) DGKs have 7.9-19.2% 2-MGK activity compared to their DGK activities, whereas their 1-MGK activities are below 3.0%. Both the 1-MGK and 2-MGK activities of the type IV DGKs (zeta and iota) are below 1% relative to their DGK activities. Type V DGKtheta has approximately 6% 1-MGK activity and below 2% 2-MGK activity compared to its DGK activity. Purified DGKtheta exhibits the same results, indicating that its 1-MGK activity is intrinsic. DGK isozymes are categorized into three types with respect to their 1-MGK and 2-MGK activities: those having (1) 2-MGK activity relatively stronger than their 1-MGK activity (types I-III), (2) only negligible 1-MGK and 2-MGK activities (type IV), and (3) 1-MGK activity stronger than its 2-MGK activity (type V). The 1-MGK activity of DGKtheta and the 2-MGK activity of DGKalpha are stronger than those of the acylglycerol kinase reported as 1-MGK and 2-MGK to date Rattus norvegicus
evolution comprehensive analysis on the 1-monoacylglycerol kinase (1-MGK) and 2-monoacylglycerol kinase (2-MGK) activities, EC 2.7.1.94, of ten diacylglyceol kinase (DGK) isozymes, EC 2.7.1.107, from different organisms. Type I (alpha, beta, and gamma), type II (delta, eta, and kappa) and type III (epsilon) DGKs have 7.9-19.2% 2-MGK activity compared to their DGK activities, whereas their 1-MGK activities are below 3.0%. Both the 1-MGK and 2-MGK activities of the type IV DGKs (zeta and iota) are below 1% relative to their DGK activities. Type V DGKtheta has approximately 6% 1-MGK activity and below 2% 2-MGK activity compared to its DGK activity. Purified DGKtheta exhibits the same results, indicating that its 1-MGK activity is intrinsic. DGK isozymes are categorized into three types with respect to their 1-MGK and 2-MGK activities: those having (1) 2-MGK activity relatively stronger than their 1-MGK activity (types I–III), (2) only negligible 1-MGK and 2-MGK activities (type IV), and (3) 1-MGK activity stronger than its 2-MGK activity (type V). The 1-MGK activity of DGKtheta and the 2-MGK activity of DGKalpha are stronger than those of the acylglycerol kinase reported as 1-MGK and 2-MGK to date Homo sapiens
malfunction presence or absence of 1-MGK and 2-MGK activities may be essential to the pathophysiological functions of each DGK isozyme Homo sapiens
malfunction presence or absence of 1-MGK and 2-MGK activities may be essential to the pathophysiological functions of each DGK isozyme Sus scrofa
malfunction presence or absence of 1-MGK and 2-MGK activities may be essential to the pathophysiological functions of each DGK isozyme Rattus norvegicus