Cloned (Comment) | Organism |
---|---|
recombinant expression of human His6-tagged DGK isozyme theta from vector pSF-CMV-NH2-His-EKT3 in COS-7 cells | Homo sapiens |
recombinant expression of human p3×FLAG-CMV-DGK isozyme delta2 in COS-7 cells | Homo sapiens |
recombinant expression of human p3×FLAG-CMV-DGK isozyme epsilon in COS-7 cells | Homo sapiens |
recombinant expression of human p3×FLAG-CMV-DGK isozyme eta1 in COS-7 cells | Homo sapiens |
recombinant expression of human p3×FLAG-CMV-DGK isozyme gamma in COS-7 cells | Homo sapiens |
recombinant expression of human p3×FLAG-CMV-DGK isozyme iota1 in COS-7 cells | Homo sapiens |
recombinant expression of human p3×FLAG-CMV-DGK isozyme kappa in COS-7 cells | Homo sapiens |
recombinant expression of human p3×FLAG-CMV-DGK isozyme zeta1 in COS-7 cells | Homo sapiens |
recombinant expression of pig p3×FLAG-CMV-DGK isozyme alpha in COS-7 cells | Sus scrofa |
recombinant expression of rat p3×FLAG-CMV-DGK isozyme beta in COS-7 cells | Rattus norvegicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Homo sapiens | |
Mg2+ | required | Sus scrofa | |
Mg2+ | required | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | O75912 | isozyme iota1 | - |
Homo sapiens | P49619 | isozyme gamma; isozyme gamma | - |
Homo sapiens | P52429 | isozyme epsilon; isozyme epsilon | - |
Homo sapiens | P52824 | isozyme theta | - |
Homo sapiens | Q13574 | isozyme zeta1 | - |
Homo sapiens | Q16760 | isozyme delta2; isozyme delta2 | - |
Homo sapiens | Q5KSL6 | isozyme kappa; isozyme kappa | - |
Homo sapiens | Q86XP1 | isozyme eta1; isozyme eta1 | - |
Rattus norvegicus | P68403 | isozyme beta; isozyme beta | - |
Sus scrofa | P20192 | isozyme alpha; isozyme alpha | - |
Purification (Comment) | Organism |
---|---|
recombinant human His6-tagged DGK isozyme theta from COS-7 cells by nickel affinity chromatography | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme also shows 1-monoacylglycerol kinase (1-MGK) and low 2-monoacylglycerol kinase (2-MGK) activities, cf. EC 2.7.1.94 | Homo sapiens | ? | - |
? | |
additional information | the enzyme also shows 1-monoacylglycerol kinase (1-MGK) and low 2-monoacylglycerol kinase (2-MGK) activities, cf. EC 2.7.1.94 | Sus scrofa | ? | - |
? | |
additional information | the enzyme also shows 1-monoacylglycerol kinase (1-MGK) and low 2-monoacylglycerol kinase (2-MGK) activities, cf. EC 2.7.1.94 | Rattus norvegicus | ? | - |
? | |
additional information | the enzyme also shows low 1-monoacylglycerol kinase (1-MGK) and low 2-monoacylglycerol kinase (2-MGK) activities, cf. EC 2.7.1.94 | Homo sapiens | ? | - |
? | |
additional information | the enzyme also shows negligible 1-monoacylglycerol kinase (1-MGK) and 2-monoacylglycerol kinase (2-MGK) activities, cf. EC 2.7.1.94 | Homo sapiens | ? | - |
? | |
additional information | the enzyme also shows negligible 1-monoacylglycerol kinase (1-MGK) and low 2-monoacylglycerol kinase (2-MGK) activities, cf. EC 2.7.1.94 | Homo sapiens | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Homo sapiens |
30 | - |
assay at | Sus scrofa |
30 | - |
assay at | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
assay at | Homo sapiens |
7.2 | - |
assay at | Sus scrofa |
7.2 | - |
assay at | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Homo sapiens | |
ATP | - |
Sus scrofa | |
ATP | - |
Rattus norvegicus |
General Information | Comment | Organism |
---|---|---|
evolution | comprehensive analysis on the 1-monoacylglycerol kinase (1-MGK) and 2-monoacylglycerol kinase (2-MGK) activities, EC 2.7.1.94, of ten diacylglyceol kinase (DGK) isozymes, EC 2.7.1.107, from different organisms. Type I (alpha, beta, and gamma), type II (delta, eta, and kappa) and type III (epsilon) DGKs have 7.9-19.2% 2-MGK activity compared to their DGK activities, whereas their 1-MGK activities are below 3.0%. Both the 1-MGK and 2-MGK activities of the type IV DGKs (zeta and iota) are below 1% relative to their DGK activities. Type V DGKtheta has approximately 6% 1-MGK activity and below 2% 2-MGK activity compared to its DGK activity. Purified DGKtheta exhibits the same results, indicating that its 1-MGK activity is intrinsic. DGK isozymes are categorized into three types with respect to their 1-MGK and 2-MGK activities: those having (1) 2-MGK activity relatively stronger than their 1-MGK activity (types I-III), (2) only negligible 1-MGK and 2-MGK activities (type IV), and (3) 1-MGK activity stronger than its 2-MGK activity (type V). The 1-MGK activity of DGKtheta and the 2-MGK activity of DGKalpha are stronger than those of the acylglycerol kinase reported as 1-MGK and 2-MGK to date | Homo sapiens |
evolution | comprehensive analysis on the 1-monoacylglycerol kinase (1-MGK) and 2-monoacylglycerol kinase (2-MGK) activities, EC 2.7.1.94, of ten diacylglyceol kinase (DGK) isozymes, EC 2.7.1.107, from different organisms. Type I (alpha, beta, and gamma), type II (delta, eta, and kappa) and type III (epsilon) DGKs have 7.9-19.2% 2-MGK activity compared to their DGK activities, whereas their 1-MGK activities are below 3.0%. Both the 1-MGK and 2-MGK activities of the type IV DGKs (zeta and iota) are below 1% relative to their DGK activities. Type V DGKtheta has approximately 6% 1-MGK activity and below 2% 2-MGK activity compared to its DGK activity. Purified DGKtheta exhibits the same results, indicating that its 1-MGK activity is intrinsic. DGK isozymes are categorized into three types with respect to their 1-MGK and 2-MGK activities: those having (1) 2-MGK activity relatively stronger than their 1-MGK activity (types I-III), (2) only negligible 1-MGK and 2-MGK activities (type IV), and (3) 1-MGK activity stronger than its 2-MGK activity (type V). The 1-MGK activity of DGKtheta and the 2-MGK activity of DGKalpha are stronger than those of the acylglycerol kinase reported as 1-MGK and 2-MGK to date | Sus scrofa |
evolution | comprehensive analysis on the 1-monoacylglycerol kinase (1-MGK) and 2-monoacylglycerol kinase (2-MGK) activities, EC 2.7.1.94, of ten diacylglyceol kinase (DGK) isozymes, EC 2.7.1.107, from different organisms. Type I (alpha, beta, and gamma), type II (delta, eta, and kappa) and type III (epsilon) DGKs have 7.9-19.2% 2-MGK activity compared to their DGK activities, whereas their 1-MGK activities are below 3.0%. Both the 1-MGK and 2-MGK activities of the type IV DGKs (zeta and iota) are below 1% relative to their DGK activities. Type V DGKtheta has approximately 6% 1-MGK activity and below 2% 2-MGK activity compared to its DGK activity. Purified DGKtheta exhibits the same results, indicating that its 1-MGK activity is intrinsic. DGK isozymes are categorized into three types with respect to their 1-MGK and 2-MGK activities: those having (1) 2-MGK activity relatively stronger than their 1-MGK activity (types I-III), (2) only negligible 1-MGK and 2-MGK activities (type IV), and (3) 1-MGK activity stronger than its 2-MGK activity (type V). The 1-MGK activity of DGKtheta and the 2-MGK activity of DGKalpha are stronger than those of the acylglycerol kinase reported as 1-MGK and 2-MGK to date | Rattus norvegicus |
evolution | comprehensive analysis on the 1-monoacylglycerol kinase (1-MGK) and 2-monoacylglycerol kinase (2-MGK) activities, EC 2.7.1.94, of ten diacylglyceol kinase (DGK) isozymes, EC 2.7.1.107, from different organisms. Type I (alpha, beta, and gamma), type II (delta, eta, and kappa) and type III (epsilon) DGKs have 7.9-19.2% 2-MGK activity compared to their DGK activities, whereas their 1-MGK activities are below 3.0%. Both the 1-MGK and 2-MGK activities of the type IV DGKs (zeta and iota) are below 1% relative to their DGK activities. Type V DGKtheta has approximately 6% 1-MGK activity and below 2% 2-MGK activity compared to its DGK activity. Purified DGKtheta exhibits the same results, indicating that its 1-MGK activity is intrinsic. DGK isozymes are categorized into three types with respect to their 1-MGK and 2-MGK activities: those having (1) 2-MGK activity relatively stronger than their 1-MGK activity (types IIII), (2) only negligible 1-MGK and 2-MGK activities (type IV), and (3) 1-MGK activity stronger than its 2-MGK activity (type V). The 1-MGK activity of DGKtheta and the 2-MGK activity of DGKalpha are stronger than those of the acylglycerol kinase reported as 1-MGK and 2-MGK to date | Homo sapiens |
malfunction | presence or absence of 1-MGK and 2-MGK activities may be essential to the pathophysiological functions of each DGK isozyme | Homo sapiens |
malfunction | presence or absence of 1-MGK and 2-MGK activities may be essential to the pathophysiological functions of each DGK isozyme | Sus scrofa |
malfunction | presence or absence of 1-MGK and 2-MGK activities may be essential to the pathophysiological functions of each DGK isozyme | Rattus norvegicus |