Protein Variants | Comment | Organism |
---|---|---|
L447 | residue is required for the cholesterol recognition/interaction amino acid consensus motif, mutation results in a loss of enzymatic activity | Homo sapiens |
L447I | ratio of enzymic activity with substrates 1-stearoyl-2-linoleoyl-sn-glycerol to 1-stearoyl-2-arachidonoyl-sn-glycerol is 0.054 | Homo sapiens |
R457K | ratio of enzymic activity with substrates 1-stearoyl-2-linoleoyl-sn-glycerol to 1-stearoyl-2-arachidonoyl-sn-glycerol is 0.217 | Homo sapiens |
R457Q | mutation results in the loss of the cholesterol recognition/interaction amino acid consensus motif and the loss of a positively charged residue, resulting in a higher enzymatic activity than wild-type. Ratio of enzymic activity with substrates 1-stearoyl-2-linoleoyl-sn-glycerol to 1-stearoyl-2-arachidonoyl-sn-glycerol is 0.099. Mutant gains preference for substrate 1-stearoyl-2-docosahexaenoyl-sn-glycerol | Homo sapiens |
Y451F | mutation results in a loss of a hydroxyl group and an essential residue of the cholesterol recognition/interaction amino acid consensus motif, leading to a higher activity than the wild-type protein. Ratio of enzymic activity with substrates 1-stearoyl-2-linoleoyl-sn-glycerol to 1-stearoyl-2-arachidonoyl-sn-glycerol is 0.107. Mutant gains preference for substrate 1,2-diarachidonoyl-sn-glycerol, with activities comparable to 1-stearoyl-2-arachidonoyl-sn-glycerol | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Km value of wild-type, 2.35, mutant Y451, 2.07, mutant R457Q, 1.71 mol% 1-stearoyl-2-arachidonoyl-sn-glycerol, respectively, pH 7.2, 22°C | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P52429 | isoform diacylglycerol kinase epsilon | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 1,2-diarachidonoyl-sn-glycerol | - |
Homo sapiens | ADP + 1,2-diarachidonoyl-sn-glycerol 3-phosphate | - |
? | |
ATP + 1,2-dilinoleoyl-sn-glycerol | - |
Homo sapiens | ADP + 1,2-dilinoleoyl-sn-glycerol | - |
? | |
ATP + 1,2-dioleoyl-sn-glycerol | - |
Homo sapiens | ADP + 1,2-dioleoyl-sn-glycerol 3-phosphate | - |
? | |
ATP + 1-stearoyl-2-arachidonoyl-sn-glycerol | in wild-type, ratio of enzymic activity of substrates 1-stearoyl-2-linoleoyl-sn-glycerol to 1-stearoyl-2-arachidonoyl-sn-glycerol is 0.109 | Homo sapiens | ADP + 1-stearoyl-2-arachidonoyl-sn-glycerol 3-phosphate | - |
? | |
ATP + 1-stearoyl-2-docosahexaenoyl-sn-glycerol | no substrate for wild-type, but substrate for mutant R457Q | Homo sapiens | ADP + 1-stearoyl-2-docosahexaenoyl-sn-glycerol 3-phosphate | - |
? | |
ATP + 1-stearoyl-2-linoleoyl-sn-glycerol | in wild-type, ratio of enzymic activity of substrates 1-stearoyl-2-linoleoyl-sn-glycerol to 1-stearoyl-2-arachidonoyl-sn-glycerol is 0.109 | Homo sapiens | ADP + 1-stearoyl-2-linoleoyl-sn-glycerol 3-phosphate | - |
? | |
additional information | the cholesterol recognition/interaction amino acid consensus domain adjacent to the lipoxygenase-like motif plays a role in acyl-chain selectivity. Despite the high degree of conservation of the amino acid sequence in this region of the protein, certain mutations result in proteins with higher activity than the wild-type protein. These mutations also result in a selective gain of acyl-chain preferences for diacylglycerols with different acyl-chain profiles. In addition to the lipoxygenase-like motif, adjacent residues also contribute to selectivity for diacylglycerols with specific acyl-chain compositions | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DGKE | - |
Homo sapiens |