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Literature summary for 2.7.1.107 extracted from

  • DSouza, K.; Epand, R.M.
    Catalytic activity and acyl-chain selectivity of diacylglycerol kinase epsilon are modulated by residues in and near the lipoxygenase-like motif (2012), J. Mol. Biol., 416, 619-628.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
L447 residue is required for the cholesterol recognition/interaction amino acid consensus motif, mutation results in a loss of enzymatic activity Homo sapiens
L447I ratio of enzymic activity with substrates 1-stearoyl-2-linoleoyl-sn-glycerol to 1-stearoyl-2-arachidonoyl-sn-glycerol is 0.054 Homo sapiens
R457K ratio of enzymic activity with substrates 1-stearoyl-2-linoleoyl-sn-glycerol to 1-stearoyl-2-arachidonoyl-sn-glycerol is 0.217 Homo sapiens
R457Q mutation results in the loss of the cholesterol recognition/interaction amino acid consensus motif and the loss of a positively charged residue, resulting in a higher enzymatic activity than wild-type. Ratio of enzymic activity with substrates 1-stearoyl-2-linoleoyl-sn-glycerol to 1-stearoyl-2-arachidonoyl-sn-glycerol is 0.099. Mutant gains preference for substrate 1-stearoyl-2-docosahexaenoyl-sn-glycerol Homo sapiens
Y451F mutation results in a loss of a hydroxyl group and an essential residue of the cholesterol recognition/interaction amino acid consensus motif, leading to a higher activity than the wild-type protein. Ratio of enzymic activity with substrates 1-stearoyl-2-linoleoyl-sn-glycerol to 1-stearoyl-2-arachidonoyl-sn-glycerol is 0.107. Mutant gains preference for substrate 1,2-diarachidonoyl-sn-glycerol, with activities comparable to 1-stearoyl-2-arachidonoyl-sn-glycerol Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information Km value of wild-type, 2.35, mutant Y451, 2.07, mutant R457Q, 1.71 mol% 1-stearoyl-2-arachidonoyl-sn-glycerol, respectively, pH 7.2, 22°C Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens P52429 isoform diacylglycerol kinase epsilon
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 1,2-diarachidonoyl-sn-glycerol
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Homo sapiens ADP + 1,2-diarachidonoyl-sn-glycerol 3-phosphate
-
?
ATP + 1,2-dilinoleoyl-sn-glycerol
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Homo sapiens ADP + 1,2-dilinoleoyl-sn-glycerol
-
?
ATP + 1,2-dioleoyl-sn-glycerol
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Homo sapiens ADP + 1,2-dioleoyl-sn-glycerol 3-phosphate
-
?
ATP + 1-stearoyl-2-arachidonoyl-sn-glycerol in wild-type, ratio of enzymic activity of substrates 1-stearoyl-2-linoleoyl-sn-glycerol to 1-stearoyl-2-arachidonoyl-sn-glycerol is 0.109 Homo sapiens ADP + 1-stearoyl-2-arachidonoyl-sn-glycerol 3-phosphate
-
?
ATP + 1-stearoyl-2-docosahexaenoyl-sn-glycerol no substrate for wild-type, but substrate for mutant R457Q Homo sapiens ADP + 1-stearoyl-2-docosahexaenoyl-sn-glycerol 3-phosphate
-
?
ATP + 1-stearoyl-2-linoleoyl-sn-glycerol in wild-type, ratio of enzymic activity of substrates 1-stearoyl-2-linoleoyl-sn-glycerol to 1-stearoyl-2-arachidonoyl-sn-glycerol is 0.109 Homo sapiens ADP + 1-stearoyl-2-linoleoyl-sn-glycerol 3-phosphate
-
?
additional information the cholesterol recognition/interaction amino acid consensus domain adjacent to the lipoxygenase-like motif plays a role in acyl-chain selectivity. Despite the high degree of conservation of the amino acid sequence in this region of the protein, certain mutations result in proteins with higher activity than the wild-type protein. These mutations also result in a selective gain of acyl-chain preferences for diacylglycerols with different acyl-chain profiles. In addition to the lipoxygenase-like motif, adjacent residues also contribute to selectivity for diacylglycerols with specific acyl-chain compositions Homo sapiens ?
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?

Synonyms

Synonyms Comment Organism
DGKE
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Homo sapiens