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Literature summary for 2.7.1.107 extracted from

  • Knight, M.J.; Joubert, M.K.; Plotkowski, M.L.; Kropat, J.; Gingery, M.; Sakane, F.; Merchant, S.S.; Bowie, J.U.
    Zinc binding drives sheet formation by the SAM domain of diacylglycerol kinase delta (2010), Biochemistry, 49, 9667-9676.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
transfection of HEK-293 cells, COS-7 cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
C20A mutant shows diminished Zn occupancy Homo sapiens
C60A mutant shows diminished Zn occupancy Homo sapiens
E35G mutant exhibits greatly reduced polymerization. Samples of the mutant incubated with an excess of zinc are shifted entirely to the insoluble fraction. In absence of zinc, most of the mutant protein sample is monomeric. In the presence of added zinc, the mutant organizes into large sheet structures Homo sapiens
H16A mutant shows diminished Zn occupancy Homo sapiens
H38A mutant shows diminished Zn occupancy Homo sapiens
H3A mutant shows diminished Zn occupancy Homo sapiens
H3A/C20A/H38A/C60A mutant shows a reduced zinc retention of 3%. Construct does not show any increase in turbidity after incubation with 50 microM zinc acetate. In the absence of zinc, short polymers are observed, much like the wild-type protein. When zinc is added, polymers increase in prevalence and length marginally but no large sheet structures are formed in 50 microM zinc. Mutant diminishes the formation of cytoplasmic puncta, shows partially impaired regulation of transport to the plasma membrane, and lacks the ability to inhibit the formation of CopII coated vesicles Homo sapiens
V52E mutant exhibits greatly reduced polymerization, no polymers are visible in zinc-free conditions. After zinc addition, large sheet structures appear Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Zinc the sterile alpha motif domain of diacylglycerol kinase delta binds zinc at multiple sites, driving the organization of the enzyme into large sheets of polymers. A mutant enzyme containing a sterile alpha motif domain refractory to zinc binding diminishes the formation of cytoplasmic puncta, shows partially impaired regulation of transport to the plasma membrane, and lacks the ability to inhibit the formation of CopII coated vesicles Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
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isoform diacylglycerol kinase delta
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