C20A |
mutant shows diminished Zn occupancy |
Homo sapiens |
C60A |
mutant shows diminished Zn occupancy |
Homo sapiens |
E35G |
mutant exhibits greatly reduced polymerization. Samples of the mutant incubated with an excess of zinc are shifted entirely to the insoluble fraction. In absence of zinc, most of the mutant protein sample is monomeric. In the presence of added zinc, the mutant organizes into large sheet structures |
Homo sapiens |
H16A |
mutant shows diminished Zn occupancy |
Homo sapiens |
H38A |
mutant shows diminished Zn occupancy |
Homo sapiens |
H3A |
mutant shows diminished Zn occupancy |
Homo sapiens |
H3A/C20A/H38A/C60A |
mutant shows a reduced zinc retention of 3%. Construct does not show any increase in turbidity after incubation with 50 microM zinc acetate. In the absence of zinc, short polymers are observed, much like the wild-type protein. When zinc is added, polymers increase in prevalence and length marginally but no large sheet structures are formed in 50 microM zinc. Mutant diminishes the formation of cytoplasmic puncta, shows partially impaired regulation of transport to the plasma membrane, and lacks the ability to inhibit the formation of CopII coated vesicles |
Homo sapiens |
V52E |
mutant exhibits greatly reduced polymerization, no polymers are visible in zinc-free conditions. After zinc addition, large sheet structures appear |
Homo sapiens |