Activating Compound | Comment | Organism | Structure |
---|---|---|---|
dioleoyl-phosphatidylglycerol | - |
Staphylococcus aureus |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Staphylococcus aureus |
Protein Variants | Comment | Organism |
---|---|---|
D124A | mutant shows strongly reduced activity | Staphylococcus aureus |
D216A | mutant shows strongly reduced activity | Staphylococcus aureus |
D271A | mutant shows almost no activity | Staphylococcus aureus |
D68A | mutant shows almost no activity | Staphylococcus aureus |
D97A | mutant shows strongly reduced activity | Staphylococcus aureus |
E273A | mutant shows almost no activity | Staphylococcus aureus |
K15A | mutant shows reduced activity | Staphylococcus aureus |
K15A/K165A | mutant shows strongly reduced activity | Staphylococcus aureus |
K165A | mutant shows reduced activity | Staphylococcus aureus |
N96A | mutant shows strongly reduced activity | Staphylococcus aureus |
R100A | mutant shows reduced activity | Staphylococcus aureus |
R20A | mutant shows wild type activity | Staphylococcus aureus |
T94A | mutant shows strongly reduced activity | Staphylococcus aureus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Staphylococcus aureus | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required for activity, interfacial binding of DgkB requires a Mg2+-dependent conformational change | Staphylococcus aureus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Staphylococcus aureus | Q6GFF9 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Staphylococcus aureus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0004 | - |
mutant enzyme D271A, specific activity is less than 0.0004 micromol/min/mg, in 50 mM HEPES, pH 7.4, 150 mM LiCl, 10 mM MgCl2, 5 mM ATP, and lipid vesicles | Staphylococcus aureus |
0.0004 | - |
mutant enzyme D68A, specific activity is less than 0.0004 micromol/min/mg, in 50 mM HEPES, pH 7.4, 150 mM LiCl, 10 mM MgCl2, 5 mM ATP, and lipid vesicles | Staphylococcus aureus |
0.0004 | - |
mutant enzyme E273A, specific activity is less than 0.0004 micromol/min/mg, in 50 mM HEPES, pH 7.4, 150 mM LiCl, 10 mM MgCl2, 5 mM ATP, and lipid vesicles | Staphylococcus aureus |
0.0023 | - |
mutant enzyme N96A, in 50 mM HEPES, pH 7.4, 150 mM LiCl, 10 mM MgCl2, 5 mM ATP, and lipid vesicles | Staphylococcus aureus |
0.0024 | - |
mutant enzyme D124A, in 50 mM HEPES, pH 7.4, 150 mM LiCl, 10 mM MgCl2, 5 mM ATP, and lipid vesicles | Staphylococcus aureus |
0.0028 | - |
mutant enzyme K15A/K165A, in 50 mM HEPES, pH 7.4, 150 mM LiCl, 10 mM MgCl2, 5 mM ATP, and lipid vesicles | Staphylococcus aureus |
0.0031 | - |
mutant enzyme T94A, in 50 mM HEPES, pH 7.4, 150 mM LiCl, 10 mM MgCl2, 5 mM ATP, and lipid vesicles | Staphylococcus aureus |
0.0036 | - |
mutant enzyme D97A, in 50 mM HEPES, pH 7.4, 150 mM LiCl, 10 mM MgCl2, 5 mM ATP, and lipid vesicles | Staphylococcus aureus |
0.0062 | - |
mutant enzyme D216A, in 50 mM HEPES, pH 7.4, 150 mM LiCl, 10 mM MgCl2, 5 mM ATP, and lipid vesicles | Staphylococcus aureus |
0.0122 | - |
mutant enzyme K15A, in 50 mM HEPES, pH 7.4, 150 mM LiCl, 10 mM MgCl2, 5 mM ATP, and lipid vesicles | Staphylococcus aureus |
0.0139 | - |
mutant enzyme K165A, in 50 mM HEPES, pH 7.4, 150 mM LiCl, 10 mM MgCl2, 5 mM ATP, and lipid vesicles | Staphylococcus aureus |
0.0168 | - |
mutant enzyme R100A, in 50 mM HEPES, pH 7.4, 150 mM LiCl, 10 mM MgCl2, 5 mM ATP, and lipid vesicles | Staphylococcus aureus |
0.0249 | - |
wild type enzyme, in 50 mM HEPES, pH 7.4, 150 mM LiCl, 10 mM MgCl2, 5 mM ATP, and lipid vesicles | Staphylococcus aureus |
0.0256 | - |
mutant enzyme R20A, in 50 mM HEPES, pH 7.4, 150 mM LiCl, 10 mM MgCl2, 5 mM ATP, and lipid vesicles | Staphylococcus aureus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 1,2-diacylglycerol | - |
Staphylococcus aureus | ADP + 1,2-diacyl-sn-glycerol 3-phosphate | - |
? | |
additional information | 1,2-diacylglycerol embedded in unilamellar dioleoyl-phosphatidylcholine vesicles is not a substrate for DgkB | Staphylococcus aureus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DAG kinase | - |
Staphylococcus aureus |
DgkB | - |
Staphylococcus aureus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Staphylococcus aureus |