Crystallization (Comment) | Organism |
---|---|
isoform DgkB, both as free enzyme and in complex with ADP, 2.4 and 2.3 A resolution, respectively. Enzyme is a tight homodimer, and each monomer comprises two domains with the catalytic center located within the interdomain cleft | Staphylococcus aureus |
Protein Variants | Comment | Organism |
---|---|---|
D124A | residue involved in the Mg1-water network, no catalytic acitivity | Staphylococcus aureus |
D271A | residue involved in the Mg1-water network, no catalytic acitivity | Staphylococcus aureus |
D68A | no catalytic activity. Role of D68 in mediating the interaction of Mg2+ with the gamma-phosphate of ATP | Staphylococcus aureus |
E273A | no catalytic activity | Staphylococcus aureus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | structure reveals a Mg2+ site and an associated Asp-water-Mg2+ network | Staphylococcus aureus | |
additional information | neither Ca2+, Mn2+, nor Zn2+ is able to effectively replace Mg2+, although Ca2+ and Mn2+ support a few percent activity | Staphylococcus aureus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37333 | - |
x * 37333, calculated, x * 42000, SDS-PAGE | Staphylococcus aureus |
42000 | - |
x * 37333, calculated, x * 42000, SDS-PAGE | Staphylococcus aureus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Staphylococcus aureus | Q6GFF9 | - |
- |
Subunits | Comment | Organism |
---|---|---|
? | x * 37333, calculated, x * 42000, SDS-PAGE | Staphylococcus aureus |